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Pinealocyte
About: Pinealocyte is a research topic. Over the lifetime, 1605 publications have been published within this topic receiving 55609 citations.
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TL;DR: Changes of some ultrastructural parameters in mole pinealocytes were quantitatively studied during the various phases of the sexual cycle and seem to demonstrate close relationship between the endocrine activity of the hypophyseo-gonadal axis and the synthesis of some compounds by the pinealocyte.
Abstract: Changes of some ultrastructural parameters (paracrystalline structures, Golgi apparatus, secretory granules) in mole pinealocytes were quantitatively studied during the various phases of the sexual cycle An increase in quantity of paracrystalline structures and of the Golgi apparatuses was demonstrated during the period of high sexual activity in male pinealocytes and during oestrus, gestation and lactation in female pinealocytes Moreover, the appearance of vacuoles in female pinealocytes was observed during pro-oestrus These results seem to demonstrate close relationship between the endocrine activity of the hypophyseo-gonadal axis and the synthesis of some compounds by the pinealocytes No increase in the quantity of secretory granules was observed during the various phases of the sexual cycle studied
31 citations
TL;DR: The results indicate that the rat SCN is capable of synthesizing melatonin and suggest that melatonin synthesis in the SCN may be regulated by the circadian clock at the post transcriptional level.
31 citations
TL;DR: The results presented here demonstrate that Isl1 positively modulates melatonin synthesis by targeting AANAT, via the ERK signaling pathway of NE, and suggest that isl1 plays important roles in maintaining the daily circadian rhythm.
Abstract: Melatonin is a key hormone that regulates circadian rhythms, metabolism, and reproduction. However, the mechanisms of melatonin synthesis and secretion have not been fully defined. The purpose of this study was to investigate the functions of the LIM homeobox transcription factor Isl1 in regulating melatonin synthesis and secretion in porcine pineal gland. We found that Isl1 is highly expressed in the melatonin-producing cells in the porcine pineal gland. Further functional studies demonstrate that Isl1 knockdown in cultured primary porcine pinealocytes results in the decline of melatonin and arylalkylamine N-acetyltransferase (AANAT) mRNA levels by 29.2% and 72.2%, respectively, whereas Isl1 overexpression raised by 1.3-fold and 2.7-fold. In addition, the enhancing effect of norepinephrine (NE) on melatonin synthesis was abolished by Isl1 knockdown. The in vivo intracerebroventricular NE injections upregulate Isl1 mRNA and protein levels by about threefold and 4.5-fold in the porcine pineal gland. We then examined the changes in Isl1 expression in the pineal gland and global melatonin levels throughout the day. The results show that Isl1 protein level at 24:00 is 2.5-fold higher than that at 12:00, which is parallel to melatonin levels. We further found that Isl1 increases the activity of AANAT promoter, and the effect of NE on Isl1 expression was blocked by an ERK inhibitor. Collectively, the results presented here demonstrate that Isl1 positively modulates melatonin synthesis by targeting AANAT, via the ERK signaling pathway of NE. These suggest that Isl1 plays important roles in maintaining the daily circadian rhythm.
31 citations
TL;DR: Testing the effects of cytokines on pinealocytes and the role of pineal microglia in mediating these cytokine effects suggests that endogenous cytokines play regulatory roles in response to peripheral homeostatic changes.
Abstract: The role of the pineal gland in regulating immune function has been extensively investigated. However, there is little information about possible feedback mechanisms of immunological factors on pineal gland neuroendocrine functions. Therefore, experiments were designed to test the effects of cytokines (interferon-gamma, IFN-gamma, interleukin-1 beta, IL-1 beta; tumor necrosis factor-alpha, TNF-alpha; transforming growth factor-beta 1, TGF-beta 1) on pinealocytes and the role of pineal microglia in mediating these cytokine effects in the pineal gland of the rat. Our studies showed that IFN-gamma enhanced 5-hydroxytryptamine (5-HT) content (measured by high-performance liquid chromatography, HPLC) and increased pinealocyte process length in pineal cultures. IL-1 beta treatment decreased 5-HT content in both cell and organ culture, but exhibited no effect on pinealocyte process length. 5-HT content and process length were decreased by TNF-alpha treatment. IFN-gamma and IL-1 beta exhibited no significant effect in the absence of microglia in cell cultures. In contrast, TNF-alpha caused a further decline in 5-HT content even in the absence of microglia in the cultures. The effects of TNF-alpha were probably due to toxic effects, since an increased number of pyknotic nuclei were observed in treated cultured explants. TGF-beta 1 treatment caused aggregation of pinealocytes in cultures and suppressed process length and 5-HT content. In conclusion, cytokine effects on pinealocytes may be mediated by microglia (IFN-gamma and IL-1 beta) or act directly on pinealocytes (TNF-alpha). The presence of IL-1 beta and TGF-beta 1 protein in the pineal gland and the suppressive effect of TGF-beta 1 on pinealocytes in cultures further suggest that endogenous cytokines play regulatory roles in response to peripheral homeostatic changes.
31 citations
TL;DR: Whereas sufficient structural information now exists on cytoplasmic organelles such as synaptic ribbons and spherules, annulate lamellae, subsurface cisterns, and the several types of synaptic arrangements seen in relation to the pinealocyte soma and its processes, the functional role of these structures in pineal synthetic processes remains to be elucidated.
Abstract: Pinealocytes are not only the principal cellular components of the pineal gland, but they are also the principal synthetic machinery of this enigmatical gland with highly diverse and often questionable empyreal roles assigned to it. Ultrastructural descriptions of pinealocytes belonging to some 70 species of mammals (a mere 2% or less of the over 4,200 mammalian species) have been summarized from the available literature with new observations on 12 species of chiropterans. Space limitation precluded any treatment of the supporting glia, neural elements, and the perivascular spaces. A detailed table lists nearly all mammalian species whose pineal ultrastructure has been investigated. Blanks in this table point to the necessity of studies on those particular groups. A tabular listing of unusual structures reported within the pinealocyte cytoplasm points out the impending experimental work on these species. Such studies using the latest techniques might provide clearer insights into the functional role of the pineal gland as an important and integral component of the neuroendocrine axis. Whereas sufficient structural information now exists on cytoplasmic organelles such as synaptic ribbons and spherules, annulate lamellae, subsurface cisterns, and the several types of synaptic arrangements seen in relation to the pinealocyte soma and its processes, the functional role of these structures in pineal synthetic processes remains to be elucidated.
31 citations