Showing papers on "Piperlonguminine published in 2008"

In vivo growth inhibition of sarcoma 180 by piperlonguminine, an alkaloid amide from the Piper species.

Abstract: Many authors have already emphasized that phytochemicals from spices have biological applications Piperlonguminine is a known alkaloid amide from peppers, including Piper divaricatum The aim of this study was to investigate the in vitro and in vivo antitumor effects of piperlonguminine in experimental models In order to evaluate the toxicological aspects related to piperlonguminine treatment, hematological, biochemical, histopathological and morphological analyses of treated animals were performed Piperlonguminine did not show any significant in vitro cytotoxic effect at experimental exposure levels, but showed an in vivo antitumor effect After 7 days of treatment, the inhibition rates were 3871% and 4068% at doses of 25 mg kg(-1) and 50 mg kg(-1), respectively The histopathological analysis suggests that the liver and kidney were only weakly affected by piperlonguminine treatment Neither the enzymatic activity of transaminases (AST and ALT) nor the urea levels were significantly altered In the hematological analysis, all parameters analysed remained constant after piperlonguminine treatment In conclusion, these data reinforce the anticancer potential of spice components

Analysis by HPLC and LC/MS of pungent piperamides in commercial black, white, green, and red whole and ground peppercorns.

Abstract: Pepper plants accumulate pungent bioactive alkaloids called piperamides. To facilitate studies in this area, high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry methods were developed and used to measure the following piperamides in 10 commercial whole (peppercorns) and in 10 ground, black, white, green, and red peppers: piperanine, piperdardine, piperine, piperlonguminine, and piperettine. Structural identification of individual compounds in extracts was performed by associating the HPLC peak of each compound with the corresponding mass spectrum. The piperanine content of the peppers (in mg/g piperine equivalents) ranged from 0.3 for the ground white pepper to 1.4 in black peppercorns. The corresponding range for piperdardine was from 0.0 for seven samples to 1.8 in black peppercorns; for four isomeric piperines, from 0.7 for red to 129 in green peppercorns; for piperlonguminine, from 0.0 in red peppercorns to 1.0 in black peppercorns; and for piperyline, from 0.9 in ground black pepper to 5.9 for red peppercorn. Four well-separated stereoisomeric forms of piperettine with the same molecular weight were present in 19 peppers. The sums of the piperamides ranged from 6.6 for red to 153 for green peppercorns. In contrast to large differences in absolute concentrations among the peppers, the ratios of piperines to total piperamide were quite narrow, ranging from 0.76 for black to 0.90 for white peppercorns, with an average value of 0.84 +/- 0.04 ( n = 19). Thus, on average, the total piperamide content of the peppers consists of 84% piperines and 16% other piperamides. These results demonstrate the utility of the described extraction and analytical methods used to determine the wide-ranging individual and total piperamide contents of widely consumed peppers.

Methylpiperate derivatives from Piper longum and their inhibition of monoamine oxidase.

Abstract: We have previously reported that piperine, a known piperidine alkaloid from Piper longum, competitively inhibited mouse brain MAO-A and MAO-B activities. Piperine also showed in vivo antidepressant-like activity against the tail suspension test. In the present study, we further expanded on the identification of MAO inhibitors from the fruit of P. longum. Activity-guided fractionation of a methylene chloride soluble extract led to the isolation of three known piperine-related compounds, methylpiperate (1), guineensine (2), and piperlonguminine (3). Of these, methylpiperate (1) and guineensine (2) showed significant MAO inhibitory activities with IC50 values of 3.6 and 139.2 μM, respectively. Furthermore, methylpiperate (1) exhibited a selective inhibitory effect against MAO-B (IC50 value: 1.6 μM) than MAO-A (IC50 value: 27.1 μM). The kinetic study using the Lineweaver-Burk plots analysis suggested that methylpiperate (1) competitively inhibits MAO-A and MAO-B activities with the Ki values of 23.5 and 1.3 μM, respectively.

Effect of piperlonguminine on blood lipid metabolism and its related genes expression in hyperlipidemia rats

Abstract: Objective To observe the effects of piperlonguminine on the blood lipid metabolism and its related genes expression in hyperlipidemia rats. Methods The hyperlipidemia models induced by high-fat feeding were established. The piperlonguminine group was fed continuously with piperlonguminine (2.5, 5, and 10 mg/kg) for four weeks. At the last day, all the rats fasted for at least 12 h and the blood was drawn for the measurement of total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), apolipoproteinA1 (ApoA1), and apolipoprotein B (ApoB); The mRNA expression of low density lipoprotein cholesterol receptor (LDLR), ApoB, and 3-hydroxy 3-methylglutaryl co-enzyme A reductase (HMG-CoAR) were measured by RT-PCR. Results Compared with that of the high-fat model group, the serum TC, TG, LDL-C, ApoB levels, and the ratio of ApoB/ApoA1 were reduced (P0.05) and the serum HDL-C and ApoA1 were increased in the piperlonguminine group (P0.05). Piperlonguminine could increase hepatic LDLR mRNA levels (P0.05) and reduce ApoB mRNA levels (P0.05). But it had no significant effects on HMG-CoAR mRNA level (P0.05). Conclusion Piperlonguminine has the effect of regulating lipid metabolism. The mechanism is likely related to increasing LDLR mRNA expression and decreasing ApoB mRNA expression.

Effects of Piperlonguminine on Genes Expression of LOX-1 and VCAM-1 in the Aortic of Atherosclerosis Rabbits

Abstract: OBJECTIVE To observe the effects of piperlonguminine on mRNA expression of lectin-like oxidized low density lipoprotein receptor-1(LOX-1) and vascular cellular adhesion molecule-1(VCAM-1) in atherosclerosis rabbits.METHODS The atherosclerosis model of rabbits was established by feeding high-fat forage.32 New Zealand rabbits were divided randomly and equally into normal group,model group,piperlonguminine group and positive group.The piperlonguminine group and positive group were feed with piperlonguminine and simvastatin at the dose of 5 mg·kg-1·d-1.After 8 weeks,the mRNA expression level of LOX-1 and VCAM-1 were measured by Real-time PCR.RESULTS Compared with that of the normal group,the mRNA expression level of LOX-1 and VCAM-1 were both significantly increased in the model group.The average relative expression ratio of model group were as 16.8 and 8.1 times as that of normal group.Compared with that of the model group,the mRNA expression level of LOX-1 and VCAM-1 were both significantly reduced in the piperlonguminine group.The average relative expression ratio of piperlonguminine group were as 9.8 and 4 times as that of normal groups.Compared with the model group,mRNA expression levels of LOX-1and VCAM-1 were reduced by 42% and 51%.CONCLUSION The inhibition of LOX-1 and VCAM-1 mRNA expression by piperlonguminine in aorta of atherosclerosis rabbits may be its mechanisms for anti-atherosclerotic.