Showing papers on "Piperlonguminine published in 2010"

Isolation of piperdine from Piper nigrum and its antiproliferative activity.

Abstract: Many plant derived molecules have shown a promising effect in therapeutics. Among the plants investigated to date, one showing enormous potential is the Piperaceae. Piperine is an alkaloid found naturally in plants belonging to the pyridine group of Piperaceae family, such as Piper nigrum and Piper longum. It is widely used in various herbal cough syrups and it is also used in anti inflammatory, anti malarial, anti leukemia treatment. So the present study was aimed to extract the phytochemical compounds in different solvent system in P.longum, P. nigrum and Piper cubeba. In preliminary screening and confirmatory test it was identified as alkaloid. The methanol extract of P. nigrum, P. longumand ethanol extract of P. nigrum and ethyl acetate extract of P. cubeba showed good result in TLC so these extracts were taken for HPTLC analysis. In HPTLC analysis of P. nigrum sample showed six alkaloid bands, two alkaloid bands were similar to Piperine standard 1 and 2, the other alkaloid may be piperidine, piperettine and piperanine. In P. longum sample contain three alkaloid bands one band was similar to Piperine standard 1, the other may be piperlongumine and piperlonguminine and no alkaloid band was found in P. cubeba. The anti bacterial was tested against gram positive and negative organism using agar well diffusion method. High activity was found in P. nigrum ethanol extract against the organismSalmonella typii. The alkaloid piperdine was purified by refluxion method to check the antitumour activity which shows 51.38% of inhibition at 5 µg/ml concentration that conforms the compound piperidine to be used as anticancer drug for further mechanistic works. Key words: Alkaloids, piperine, HPTLC, antibacterial activity, purification, piperidine, HEp2 cell lines.

Amides from Piper nigrum L. with dissimilar effects on melanocyte proliferation in-vitro.

Abstract: Melanocyte proliferation stimulants are of interest as potential treatments for the depigmentary skin disorder, vitiligo. Piper nigrum L. (Piperaceae) fruit (black pepper) water extract and its main alkaloid, piperine (1), promote melanocyte proliferation in-vitro. A crude chloroform extract of P. nigrum containing piperine was more stimulatory than an equivalent concentration of the pure compound, suggesting the presence of other active components. Piperine (1), guineensine (2), pipericide (3), N-feruloyltyramine (4) and N-isobutyl-2E, 4E-dodecadienamide (5) were isolated from the chloroform extract. Their activity was compared with piperine and with commercial piperlongumine (6) and safrole (7), and synthetically prepared piperettine (8), piperlonguminine (9) and 1-(3, 4-methylenedioxyphenyl)-decane (10). Compounds 6-10 either occur in P. nigrum or are structurally related. Compounds 1, 2, 3, 8 and 9 stimulated melanocyte proliferation, whereas 4, 5, 6, 7 and 10 did not. Comparison of structures suggests that the methylenedioxyphenyl function is essential for melanocyte stimulatory activity. Only those compounds also possessing an amide group were active, although the amino component of the amide group and chain linking it to the methylenedioxyphenyl group can vary. P. nigrum, therefore, contains several amides with the ability to stimulate melanocyte proliferation. This finding supports the traditional use of P. nigrum extracts in vitiligo and provides new lead compounds for drug development for this disease.

Antifungal activity of natural and synthetic amides from Piper species

Abstract: The antifungal leaves extract from Piper scutifolium was submitted to bioactivity-guided chromatographic separation against Cladosporium cladosporioides and C. sphaerospermum yielding piperine, piperlonguminine and corcovadine as the active principles which displayed a detection limit of 1 µg. Structure-activity relationships were investigated with the preparation of twelve analogs having differences in the number of unsaturations, aromatic ring substituents and in the amide moiety. Analogs having a single double-bond and no substituent in the aromatic ring displayed higher activity, while N,N,-diethyl analogs displayed higher dose-dependent activity.

Isolation of piperdine from Piper nigrum and its antiproliferative activity

Abstract: Many plant-derived molecules have shown a promising effect in therapeutics. Among the plants investigated to date, one showing enormous potential is the Piperaceae. The present study aimed to extract the phytochemical compounds in different solvent system in Piper longum, Piper nigrum and Piper cubeba as well as testing their antibacterial and antitumour activity. HPTLC analysis of P. nigrumsample showed six alkaloid bands two alkaloid bands were similar to Piperine standard 1 and 2, the other alkaloid may be piperidine, piperettine and piperanine.P. longum sample contain three alkaloid bands one band was similar to Piperine standard 1, the other may be piperlongumine and piperlonguminine and no alkaloid band was found in P. cubeba. The anti bacterial activity was tested against gram positive and negative organism using agar well diffusion method. High activity was found in P. nigrum ethanol extract against the organism Salmonella Typhii. The alkaloid piperdine was purified by refluxion method to check the antitumour activity which shows 51.38% of inhibition at 5 µg/ml concentration that conforms the compound piperidine to be used as anticancer drug for further mechanistic works. Key words: Alkaloids, piperine, HPTLC, antibacterial activity, purification, piperidine, HEp2 cell lines.

Method for extracting PiPerlonguminine and drug combination comprising same

Abstract: The invention provides a method for extracting PPA (PiPerlonguminine), which comprises the following steps: firstly, extracting by using absolute alcohol to obtain alcohol extract; and further carrying out continuous separation by using silica gel columns for two times to obtain pure PPA crystal after crystallization. The method of the invention can remove a large amount of impurities, thus obtaining the PPA with the purity thereof being as high as 85% to 95%; moreover, the method of the invention has the advantages of low cost and high extraction efficiency, dispenses with toxic reagents in short supply and technically complex equipment and facilitates the batch production; and particularly, the PPA obtained by the method of the invention is low in toxicity and high in activity. The invention further provides a drug preparation comprising the PPA, a preparation method thereof and an application thereof in preparing drugs for treating hyperlipidaemia.