Showing papers on "Piperlonguminine published in 2013"

Anticoagulant activities of piperlonguminine in vitro and in vivo

Abstract: Piperlonguminine (PL), an important component of Piper longum fruits, is known to exhibit anti-hyperlipidemic, anti-platelet and anti-melanogenic activities. Here, the anticoagulant activities of PL were examined by monitoring activated-partial-thromboplastin-time (aPTT), prothrombin-time (PT), and the activities of thrombin and activated factor X (FXa). The effects of PL on the expressions of plasminogen activator inhibitor type 1 (PAI-1) and tissue-type plasminogen activator (t-PA) were also tested in tumor necrosis factor-α (TNF-α) activated HUVECs. The results showed that PL prolonged aPTT and PT significantly and inhibited the activities of thrombin and FXa. PL inhibited the generation of thrombin and FXa in HUVECs. In accordance with these anticoagulant activities, PL prolonged in vivo bleeding time and inhibited TNF-α induced PAI-1 production. Furthermore, PAI-1/t-PA ratio was significan- tly decreased by PL. Collectively, our results suggest that PL possesses antithrombotic activities and that the current study could provide bases for the development of new anticoagulant agents.

LC analysis and pharmacokinetic study of synthetic piperlonguminine in rat plasma after oral administration

Abstract: Only one kind of synthesized alkaloid, piperlonguminine, was used to understand the interference of the other alkaloids in pharmacokinetic study using HPLC/UV in rat plasma after oral administration. Compared with the previous report, it was clarified that mixed alkaloids such as piperine and the other extract from Piper longum Linn did not interfere with the results.

Analysis of amide compounds in different parts of Piper ovatum Vahl by high-performance liquid chromatographic.

Abstract: BACKGROUND Piper ovatum (Piperaceae) has been used in traditional medicine for the treatment of inflammations and as an analgesic. Previous studies have showed important biological activities of the extracts and amides from P. ovatum leaves. OBJECTIVE In this study, a high-performance liquid chromatographic (HPLC) method was developed and validated for quantitative determination of the amides in different parts of Piper ovatum. MATERIALS AND METHODS The analysis was carried out on a Metasil ODS column (150 × 4.6 mm, 5μm) at room temperature. HPLC conditions were as follows: acetonitrile (A), and water (B), 1.0% acetic acid. The gradient elution used was 0-30 min, 0-60% A; 30-40 min, 60% A. Flow rate used was 1.0mL/min, and detection at 280nm. RESULTS The validation using piperlonguminine, as the standard, demonstrated that the method shows linearity (linear correlation coefficient = 0.998), precision (relative standard deviation <5%) and accuracy (mean recovery = 103.78%) in the concentration range 31.25 - 500μg/mL. The limit of detection and quantification were 1.21 and 4.03μg/mL, respectively. This method allowed the identification and quantification of piperlonguminine and piperovatine in the hydroethanolic extracts of P. ovatum obtained from the leaves, stems and roots. All the extracts showed the same chromatographic profile. The leaves and roots contained the highest concentrations of piperlonguminine and the stems and leaves showed the most concentrations of piperovatine. CONCLUSION This HPLC method is suitable for routine quantitative analysis of amides in extracts of Piper ovatum and phytopharmaceuticals containing this herb.

Antihyperlipidemic activity of seed extract of Piper attenuatum in triton X- 100 induced hyperlipidemia in rats

Abstract: Piper attenuatum is a potent medicinal plant in the Indian systems of medicine. Piper attenuatum havin g Piperine, Piperlonguminine and other active constituents whic h are used as muscle relaxant, central nervous syst em depressant, in headache and as insecticide agent ag ainst Musca domestica. Piperine reduced the total b ody temperature and display analgesic, and anti-inflamm atory activities. The aim of present study is to de termine the Anti-Hyperlipidemic activity of Ethanolic seed extr act of plant Piper attenuatum (Linn.) B. Ham. (Fam. Piperaceae). Piperine was the first amide to be isolated from Pi per species. Ethanolic extracts of Piper attenuatum with a dose of 200 mg/kg exhibited significant Anti-Hyperlipidemic activity in Triton X-100 Induced Hyperlipidemia in rats (P<.05). It was found that Piperine & Piperlongumin ine the active constituent of the plant was respons ible for the Anti-Hyperlipidemic activity because this constitue nt have the ability to reduce the Total Cholesterol & Total Triglyceride level in rats. Fenofibrate was used as standard drug (65 mg/kg). The total time period of this study was one week.

Bioautography to assess antibacterial activity of Ottonia martiana Miq. (Piperaceae) on the human oral microbiota

Abstract: Ottonia martianaMiq. (Piperaceae), a plant known popularly in southern Brazil as “anestesia” and used in the treatment of odontalgia for its anesthetic action on the oral mucosa, was investigated for antibacterial activity by paper disc agar diffusion and bioautographic methods, against microorganisms present in the human oral cavity [Streptococcus mutans(ATCC 25175), Streptococcus mitis (ATCC 49456), Streptococcus pyogenes (ATCC 19615), Streptococcus salivarius (ATCC 25975), Escherichia coli (ATCC 11229 and 25922), Pseudomonas aeruginosa(ATCC 27853) and Enterobacter aerogenes (ATCC 27853).The crude extract of O. martiana(32.9 mg mL -1 ) had antibacterial potential against all Gram-positive bacteria tested. Analysis of the bioautograms led to the detection of bioactive substances,among which it was possible to identify piperovatine (Rf 0.35), piperlonguminine (Rf 0.52) and isopiperlonguminine (Rf 0.52). The piperovatine and isopiperlonguminine were isolated from the roots of O. martiana,guided by a bioautographic antibacterial bioassay. Keywords: Amides. Antibacterial activity. Bioautography. Toothache. Ottonia martiana. RESUMO Bioautografia para avaliacao da atividade antibacteriana da Ottonia martiana Miq. (Piperaceae) sobre a microbiota oral humana Ottonia martianaMiq. (Piperaceae), planta conhecida popularmente por “anestesia” e empregada no tratamento de odontalgias devido a sua acao anestesica sobre a mucosa oral, foi investigada por meio de ensaios antibacterianos de difusao em disco de papel e de bioautografia frente a microorganismos presentes na microbiota oral humana [Streptococcus mutans(ATCC 25175), Streptococcus mitis (ATCC 49456), Streptococcus pyogenes (ATCC 19615), Streptococcus salivarius (ATCC 25975), Escherichia coli (ATCC 11229 e 25922), Pseudomonas aeruginosa(ATCC 27853), Enterobacter aerogenes (ATCC 27853). Os resultados dos bioensaios mostraram que o extrato bruto de O. martiana(32.9 mg mL-1 ) apresenta potencial antibacteriano frente as bacterias Gram-positivas testadas. Dentre as substâncias bioativas detectadas foram identificadas a piperovatina (Rf 0.35), piperlonguminina (Rf 0.52) e a isopiperlonguminina (Rf 0.52). A piperovatina e isopiperlonguminina foram isoladadas do extrato das raizes de O. martiana, guiadas pelo teste de bioautografia. Palavras-Chave: Amidas. Atividade antibacteriana. Bioautografia. Odontalgia. Ottonia martiana.