Showing papers on "Polygonum published in 2011"

Isolation, identification and determination of methyl caffeate, ethyl caffeate and other phenolic compounds from Polygonum amplexicaule var. sinense

Abstract: Polygonum amplexicaule D. Don (Polygonaceae) (Chinese name: Xuesanqi) is a widely distributed medicinal plant in China. It has been used in folk medicine to treat many common and frequently-occurring diseases, such as cardiovascular and cerebrovascular diseases, fractures, pain, etc. Thus, this plant has been of interest to researchers. However, the chemical constituents of Xuesanqi have not been investigated systematically. This study isolated and identified some effective compounds in this plant. The structure of important active chemicals of Xuesanqi from different regions was determined for the first time. Twelve phenolic compounds were isolated from this plant. Vanillin, isovanillic acid, p-hydroxyphenethyl alcohol, dihydro-kaempferol, and 5,7-dihydroxychromone were determined for the first time fromPolygonum. As markers for quality standard of this herb, the content of methyl caffeate and ethyl caffeate was determined by HPLC. It was found that Xuesanqi from Wufeng, Lichuan, Badong Counties in Hubei Province and Jiujiang City of Jiangxi Province had higher content of both chemicals. The results suggest that the herb might be a potential source of phenolic compounds of an agent for improving blood circulation, treating various fractures, muscle and tissue swelling, and pain. Key words: Chemical constituents, Xuesanqi, analysis, high-performance liquid chromatography (HPLC).

In vitro free radicals scavenging activities of polysaccharide from Polygonum Multiflorum Thunb

Abstract: The crude polysaccharide (PMTP) was isolated from Polygonum Multiflorum Thunb through hot water extraction followed by ethanol precipitation. Through antioxidant assay in vitro, the results exhibited PMTP has powerful scavenging abilities, especially on ABTS, DPPH and Hydroxyl radicals. Therefore, the crude polysaccharide from P. Multiflorum Thunb should be explored as a novel potential antioxidant.

Quality Assessment of Polygonum cuspidatum and Polygonum multiflorum by 1H NMR Metabolite Fingerprinting and Profiling Analysis.

Abstract: The quality assessment and control of traditional Chinese medicines (TCM) nowadays receives a great deal of attention worldwide and particularly in Europe with its increasing local use. Polygonum cuspidatum Siebold & Zucc. and Polygonum multiflorum Thunb. are two members of the Polygonaceae family, which are widely used as Chinese medicinal plants. The aim of this study was to achieve an overview of the quality of P. cuspidatum and P. multiflorum samples available on the Chinese market and to identify important metabolites for their discrimination, using (1)H NMR-based metabolomics. (1)H NMR and multivariate analysis techniques were applied to almost 60 plant samples collected in different places in China. Using (1)H NMR metabolomics, it was possible, without previous evaporation or separation steps, to obtain metabolic fingerprints to distinguish between the species. The important metabolites for discrimination were stilbene derivatives. Finally, a clear distinction between the two species was possible and the discriminant metabolites were identified.

Effects of polygonum aviculare herbal extract on proliferation and apoptotic gene expression of mcf-7

Abstract: Background and the purpose of the study: One of the most common malignancies in women is breast cancer. Although several treatments for breast cancer are available, application of herbal medicine as a supplementary treatment is a new option to help curing the disease. In this study anticancer effects of Polygonum avicular herbal extract was investigated. Methods: Polygonum avicular extract was obtained by methanol. MCF-7 cell line was treated with different concentrations of Polygonum avicular (50, 100, 150, 200, 250, 300,350 400 ng/µl) for different time lengths ( 6, 12, 24, and 48 hrs). MTT assay and Flow Cytometry were used to evaluate cell proliferation and apoptosis, respectively. RT-PCR was also carried out to evaluate the expression of apoptotic genes. Results and Discussion: Results showed that Polygonum avicular induced cytotoxicity in MCF7 cell line at concentrations higher than 300 ng/µl and this was confirmed by the highest rate of cell death as measured by Trypan Blue and MTT assays. RT-PCR results showed up-regulation of P53 and down-regulation of Bcl-2 proteins which implied the ability of Polygonum avicular to induce apoptosis in MCF-7 cells and confirmed its anticancer property. Further studies are required to evaluate effects of the extract on other apoptotic genes.

Histochemical analysis of the root tuber of Polygonum multiflorum Thunb. (Fam. Polygonaceae).

Abstract: Authentication is the first priority in quality evaluation of Chinese herbal medicines (CHMs). The most commonly used authentication methods are morphological identification and microscopic identification. Unfortunately, these two methods cannot provide the chemical information needed to assess the quality of CHMs. In this study, a combination of fluorescence microscopy and high performance liquid chromatography–mass spectrometry (HPLC–MS) was used to analyze the chemical profiles of the tissues of the raw root tubers of Polygonum multiflorum Thunb. The results showed that the cork cells, cortex, and vessels in transverse sections of the raw root tuber of P. multiflorum fluoresced differently. Further analysis by HPLC–MS revealed that anthraquinones are mainly distributed in the cortex, and 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucopyranoside could be found in all tissues of the raw root tubers of P. multiflorum with its relative content as cork > cortex > xylem of allotype vascular bundles > xylem of central vascular bundles. Moreover, the fluorescence characteristics of the tissues from the steamed root tuber of P. multiflorum were compared and showed different fluorescence from those of raw material. From these results, it can be deduced that the root tuber of P. multiflorum with a broader cortex and fewer vascular bundles visible in a transverse section should be of better quality. The different fluorescence characteristics can be used to differentiate raw root tubers of P. multiflorum from those that have been steamed. Microsc. Res. Tech., 2010. © 2010 Wiley-Liss, Inc.

Drimane-type sesquiterpenes from Polygonum hydropiper.

Abstract: One new drimane-type sesquiterpenoid, 3 β-angeloyloxy-7-epifutronolide (1), and one new natural product, polygonumate (2), along with six known drimane-type sesquiterpenes [dendocarbin L (3), (+) winterin (4), (+) fuegin (5), changweikangic acid A (6), futronolide (7), and 7-ketoisodrimenin (8)] were isolated from the whole plant of Polygonum hydropiper Linn. Their structures were determined using spectroscopic techniques. Single-crystal X-ray diffraction study on dendocarbin L (3) and ¹³C-NMR spectroscopic data of (+) winterin (4) are described for the first time. Compound 6 was evaluated for inhibitions of α-chymotrypsin, acetylcholinesterase, and butyrylcholinesterase enzymes.

Anti-mite activity of Polygonum hydropiper L. (Polygonaceae) extracts against tea red spider mite, Oligonychus coffeae Nietner (Tetranychidae: Acarina)

Abstract: Acaricidal, antiovipositional and ovicidal activities of Polygonum hydropiper (Polygonaceae) against tea red spider mite, Oligonychus coffeae (Nietner) (Tetranychidae: Acarina) were studied under laboratory conditions. Essential chemicals of P. hydropiper obtained from crude extracts of both water and petroleum ether/acetone/methanol fractions by following cold percolation and sequential extraction methods on the basis of solvents' polarity. Ovicidal activity of P. hydropiper was observed only in petroleum ether and acetone fractions. High acaricidal activity were observed in acetone followed by petroleum ether fraction, methanol and finally by water fractions. The effect was stronger with increasing concentrations. Antiovipositional property was evident only at higher concentrations of P. hydropiper. Availability and distribution of this weed (P. hydropiper) in and around tea growing areas of sub-Himalayan region, along with its processing for the feasibility of including P. hydropiper extracts ...

Validation of the ethnopharmacological use of Polygonum persicaria for its antifungal properties.

Abstract: Polygonum L. genus (Polygonaceae) is represented in Argentina by 21 species and some of them have been used in the traditional medicine of our country to treat affections related with fungal infections, such as skin ailments and vaginal diseases. With the aim of contributing to the correct ethnopharmacological use of this genus, in the present work we describe the antifungal properties of P. persicaria (species not studied up to now) and the bio-guided isolation of the main active compounds. Results showed that dichloromethane extracts was the most active with MICs (Minimun Inhibitory Concentrations) between 31.2 - 1000 microg/mL, validating the ethnopharmacological use of P. persicaria to treat affections related with fungal infections in the Argentinean traditional medicine.

Chemotaxonomic Importance of Sesquiterpenes and Flavonoids in Five Argentinian Species of Polygonum Genus

Abstract: Leaves of five species of Polygonum genus belonging to Persicaria section were collected from the northeast and central lowlands of Argentina and their dichloromethane extracts were analyzed by GC/EM. They were investigated for the presence of four drimane-type sesquiterpenes: polygodial (1), isopolygodial (2), drimenol (3) and confertifolin (4), previously isolated from P. acuminatum; and the presence of three flavonoids: pinostrobin (5), flavokawin B (6) and cardamonin (7), previously isolated from P. persicaria. Results showed that among the five species of Persicaria section studied, two species contained sesquiterpenes 1–4 but not flavonoids 5–7, other two species contained flavonoids 5–7 but not sesquiterpenes 1–4, and only one species contained compounds 1–7. These results add evidences to a previous proposal to sub-classify the Persicaria section of Polygonum genus from a chemotaxonomic point of view. [Editor's note: This paper's References are notated within the main body of the text in ...

Effects of Medicinal herb Extracts and their Components on Steatogenic Hepatotoxicity in Sk-hep1 Cells

Abstract: Herbal medicines are widely used in many countries for the treatment of many diseases. Although the use of herb extracts as alternative medicine is growing, their toxicological properties have not been thoroughly investigated. In this study, we have investigated the effects of water and ethanol extracts of 18 herbs on the hepatic lipid metabolism and steatogenic hepatotoxicity. Ethanol extracts of Cirsium japonicum, Carthamus tinctorius, Rehmanniae glutinosa (preparata), Polygala tenuifolia, Foeniculum vulgare, Polygonum multiflorum, and Acorus gramineus and water extracts of Polygonum multiflorum and Rehmanniae glutinosa induced lipid accumulation in Sk-hep1 human hepatoma cells as determined by Nile red staining. These extracts increased the luciferase activity of sterol regulatory element (SRE) and decreased that of peroxisome proliferator response element (PPRE), indicating the possibilities of enhanced fatty acid synthesis and decreased fatty acid oxidation. To identify the components responsible for the fat accumulation, we tested 50 chemicals isolated from the nine herbs. Apigenin, luteolin, pectolinarin and lupeol from Cirsium japonicum, 8-methoxypsoralen and umbelliferone from Foeniculum vulgare and pomonic acid and jiocerebroside from Rehmanniae glutinosa significantly increased the accumulation of lipid droplets. These results suggest that ethanol extracts of Cirsium japonicum, Carthamus tinctorius, Rehmanniae glutinosa (preparata), Polygala tenuifolia, Foeniculum vulgare, Polygonum multiflorum, and Acorus gramineus and water extracts of Polygonum multiflorum and Rehmanniae glutinosa can cause fatty liver disease by decreasing β-oxidation of fatty acid and increasing lipogenesis.

Quality Characteristics of Jeungpyun Prepared with Different Ratios of polygonum multiflorum Thunb Powder

Abstract: We developed hasuo jeungpyun with added polygonum multiflorum Thunb powder to increase the functional value and flavor of traditional jeungpyun. Hasuo jeungpyun containing 0, 2, 4, 6 and 8% polygonum multiflorum Thunb powder was prepared and the moisture contents, pH, color, texture and sensory analysis of the samples were measured. With increasing polygonum multiflorum Thunb powder content, the moisture content of hasuo jeungpyun decreased, and pH increased significantly. Hasuo jeungpyun prepared with 2-4% polygonum multiflorum Thunb powder displayed significantly higher swelling power. As the level of polygonum multiflorum Thunb powder increased, the L-value decreased, whereas the a- and, b-values increased. A textural profile analysis showed that the hardness increased but cohesiveness decreased with increasing polygonum multiflorum Thunb powder content. A sensory evaluation showed the highest value for air cell uniformity, flavor, and overall acceptability of 4% hasuo jeungpyun, which suggests hasuo jeungpyun with improved sensory characteristics. In the analysis of the correlation between sensory characteristics and mechanical properties of hasuo jeungpyun, hardness and brittleness were negatively correlated with after swallow, appearance, flavor, texture, taste, and overall acceptability.

The apoptotic and cytotoxic effects of Polygonum avicular extract on Hela-S cervical cancer cell line

Abstract: Cervical cancer is the second main cause of cancer leading death in women. Therefore, targeting it, preferably with natural compounds derived from medicinal plant such as Polygonum avicular as an herbal extract have astringent properties. Herbal medicines in all regions of the developing world are widely used. Unlike the heavy use, there are very little information about the health and efficiency of the treatment by these plants. On the other hand, it’s proven that the relationship between apoptosis and cancer has been increased. In the present study the induction of apoptosis as well as its proliferation effects of polygonum avicular extract on Hela-S cervical cancer cell line has been investigated. First, total extract of Polygonum avicular was prepared by n-hexane, methanol and dichloromethane. Then cytotoxic effect of methanolic phase was studied on Hela-S cervical cancer cell line with 12, 24 and 48 h MTT assays. Finally, after determination of IC50, cells were treated with methanolic extract and Hoechest staning assay was done to measure the amount of apoptosis by the extract. Data analysis showed that methanolic extract of Polygonum avicular has dose-dependent cytotoxic effect on Hela-S cervical cancer cell line with IC50= 0.27±0.07 -0.41±0.02 mg/ml and the extract causes apoptosis with time-dependent manner. In conclusion, methanolic extract of polygonum avicular has cytotoxic and apoptotic effect on Hela-S cervical cell line and may be exploited as potential source for developing novel drugs against cervical cancer. Key words: Cervical cancer, polygonum avicular, Hela-S cervical cancer cell line, apoptosis.

Potential antitumor activity of two Polygonum species

Abstract: Two Bangladeshi Polygonum species, P. barbatum (L.) Hara var. barbata (common name 'bekhanjabaj') and P. stagninum Buch.-Ham. ex Meissn. (common name 'ratooti sag' or 'bara bishkatali'), are perennial herbs of the family Polygonaceae. The genus Polygonum L. is well-known for its use in oriental traditional medicine systems for the treat- ment of various ailments including fever, pain, inflammation, infections, cancer and tumors. The extracts of P. barbatum var. barbata and P. stagninum were assessed for potential antitumor properties using the potato disc assay. All extracts showed a considerable level of potential antitumor activity. The petroleum ether extract of P. barbatum var. barbata and the n-hexane and ethyl acetate extracts of P. stagninum, having IC50 values of 290, 200 and 180 µg/disc, respectively, were the most active among the extracts. The methanol extracts of both plants were the least active and had an IC 50 value of >400 µg/disc. Overall, the extracts of P. stagninum showed better antitumor activity potential than the extracts of P. bar- batum var. barbata.

Allelopathic Effect of some Medicinal Plants on Germination of two Dominant Weeds in Algeria

Abstract: The main objective of the present study was to investigate the phytotoxic effects of five medicinal plants; Colocynthis vulgaris Schrad, Retama retam L, Traganum nudatum Del, Pituranthos chloranthus Benth. & Hook.and Artemisia herba-alba Asso on germination efficiency, germination index and radicle length of the two dominant weeds; Avena fatua L. and Polygonum convolvulus L. in Algeria. The results showed that the aqueous root extract of the five donor species has no allelopathic effect on the measured parameters of the two weed species. On the other hand, the shoots exhibited a significant effect only on Avena fatua, while Polygonum convolvulus was highly suppressed by shoots of Colocynthis vulgaris and Traganum nadatum.

Molecular systematics of polygonum minus huds. Based on ITS sequences

Abstract: Plastid trnL-trnF and nuclear ribosomal ITS sequences were obtained from selected wild-type individuals of Polygonum minus Huds. in Peninsular Malaysia. The 380 bp trnL-trnF sequences of the Polygonum minus accessions were identical. Therefore, the trnL-trnF failed to distinguish between the Polygonum minus accessions. However, the divergence of ITS sequences (650 bp) among the Polygonum minus accessions was 1%, indicating that these accessions could be distinguished by the ITS sequences. A phylogenetic relationship based on the ITS sequences was inferred using neighbor-joining, maximum parsimony and Bayesian inference. All of the tree topologies indicated that Polygonum minus from Peninsular Malaysia is unique and different from the synonymous Persicaria minor (Huds.) Opiz and Polygonum kawagoeanum Makino.

Chemical constituents in herb of Polygonum orientale II

Abstract: OBJECTIVE To study the chemical constituents of the essential substance from the Polygonum oriental. METHOD Chromatographic techniques were employed for isolation and purification of the constituents and their structures were determined by spectral analysis and chemical evidence. RESULT Seven compounds were obtained and identified as ombuine-3-O-beta-D-galactopyranoside (1), ombuine-3-O-rutinoside (2), tryptophan (3), quercetin-3-O-methyl ether (4), kaempferol-3-O-(2"-O-alpha-L-rhamnopyranosyl) -beta-D-glucuronopyranoside (5), quercetin-3-O-(2"-O-alpha-L-rhamnopyranosyl)-beta-D-glucuronopyranoside (6), quercetin-3-O-beta-D-glucuronide (7). CONCLUSION Compounds 4-7 were isolated from P. oriental for the first time and compounds 1-3 were firstly obtained from the genus Polygonum. The total 1H and 13C-NMR date of compound 1 were assigned for first time.

Effect of Botanical Formulation of Polygonum Minus (P-40) on Control of Alternaria Solani

Abstract: An ecofriendly botanical formulation "Polymin 40 EC" was developed from the chloroform extract of P. minus. The effect of various concentrations of P-40 on seed infection, germination and vigour of tomato and 2 per cent P-40 was found to increase the germination and vigour but reduced the seed infection to a significant extent. Two percent P-40 was found to be the optimum concentration for plant disease control (tomato - Alternaria solani) under pot culture conditions. Application of P-40 increased the activity of peroxidase (PO), polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL) and phenol content of the crop plants.

Effect of clone selection, nitrogen supply, leaf damage and mycorrhizal fungi on stilbene and emodin production in knotweed

Abstract: Fallopia japonica and its hybrid, F. xbohemica, due to their fast spread, are famous as nature threats rather than blessings. Their fast growth rate, height, coverage, efficient nutrient translocation between tillers and organs and high phenolic production, may be perceived either as dangerous or beneficial features that bring about the elimination of native species or a life-supporting source. To the best of our knowledge, there have not been any studies aimed at increasing the targeted production of medically desired compounds by these remarkable plants. We designed a two-year pot experiment to determine the extent to which stilbene (resveratrol, piceatannol, resveratrolosid, piceid and astringins) and emodin contents of F. japonica, F. sachalinensis and two selected F. xbohemica clones are affected by soil nitrogen (N) supply, leaf damage and mycorrhizal inoculation. 1) Knotweeds are able to grow on substrates with extremely low nitrogen content and have a high efficiency of N translocation. The fast-spreading hybrid clones store less N in their rhizomes than the parental species. 2) The highest concentrations of stilbenes were found in the belowground biomass of F. japonica. However, because of the high belowground biomass of one clone of F. xbohemica, this hybrid produced more stilbenes per plant than F. japonica. 3) Leaf damage increased the resveratrol and emodin contents in the belowground biomass of the non-inoculated knotweed plants. 4) Although knotweed is supposed to be a non-mycorrhizal species, its roots are able to host the fungi. Inoculation with mycorrhizal fungi resulted in up to 2% root colonisation. 5) Both leaf damage and inoculation with mycorrhizal fungi elicited an increase of the piceid (resveratrol-glucoside) content in the belowground biomass of F. japonica. However, the mycorrhizal fungi only elicited this response in the absence of leaf damage. Because the leaf damage suppressed the effect of the root fungi, the effect of leaf damage prevailed over the effect of the mycorrhizal fungi on the piceid content in the belowground biomass. Two widely spread knotweed species, F. japonica and F. xbohemica, are promising sources of compounds that may have a positive impact on human health. The content of some of the target compounds in the plant tissues can be significantly altered by the cultivation conditions including stress imposed on the plants, inoculation with mycorrhizal fungi and selection of the appropriate plant clone.

Fingerprint of Polygonum multiflorum and establishment method and application thereof

Abstract: The invention discloses a quality control method of Polygonum multiflorum through the fingerprint thereof The fingerprint is HPLC (high-performance liquid chromatography) fingerprint obtained through HPLC analysis of Polygonum multiflorum The fingerprint can be used for completely monitoring the quality of Polygonum multiflorum to ensure the stable and uniform quality of Polygonum multiflorum and related pharmaceutical preparations containing Polygonum multiflorum The invention further discloses the establishment method and the application of the fingerprint of Polygonum multiflorum

Screening of antibacterial, antifungal and cytotoxic activities of Polygonum hydropiper L. stem extracts.

Abstract: The aim of the study was to investigate the antibacterial, antifungal and cytotoxic activities of ethanol extract of Polygonum hydropiper stem Disc diffusion method measuring minimum inhibitory concentration (MIC) was used to demonstrate antibacterial and antifungal activities Stem extract showed significant antibacterial activities against three gram-positive (Bacillus subtilis, Bacillus megaterium and Staphylococcus aureus) and four gram-negative (Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi and Shigella sonnei) bacteria resulting 16 to 64 g/ml MIC values against these bacteria Further, the antifungal activities of stem extract were not highly remarked but still to be considered as inhibitory to tested fungi LC50 of the extract against brine shrimp nauplii was 3546g/ml and indicates the cytotoxic potentiality of Polygonum hydropiper stem Results obtained in this study suggest that Polygonum hydropiper stem can also be used as a source of antimicrobial and cytotoxic substances for possible for plant protections

Chemical Constituents from Polygonum capitatum

Abstract: Nine compounds were isolated from EtOAc extract of the Polygonum capitatum Buch.-Ham.ex D.Don..Their structures were identified as quercetin 3-O-(4″-O-acetyl)-α-L-rhamnopyranoside(1),quercetin(2),quercitrin(3),myricetrin(4),quercetin 3-rhamnoside 2″-gallate(5),protocatechuic acid(6),daucosterol(7),gallic acid(8) and progallin A(9) on the basis of their physical properties and the spectral data.Compound 1 was isolated from the genus Polygonum for the first time,and 4 was isolated from the plant for the first time.The NMR spectroscopic characteristics of the acetylated rhamnose of flavonoids glycosides were summarized.

Genetic diversity and relationships detected by inter simple sequence repeat (ISSR) and randomly amplified polymorphic DNA (RAPD) analysis among Polygonum species growing in North of Iran

Abstract: The phylogenic relationship within four species of Polygonum (including Polygonum hyrcanicum Rech. f. (three samples), Polygonum persicaria Boiss & Bushe Boiss, Polygonum avicular L., and Polygonum hydropiper L. subsp. Hydropiper ) was investigated by randomly amplified polymorphic DNA analysis (RAPD) and inter simple sequence repeat (ISSR) markers. All the species were clearly identified using 20 RAPD primers (10-mer ZO1- ZO20) and 15 ISSR primers (S1-S15). The total number of amplification products produced with 20 primers of both markers was 315. Genetic distance was calculated in order to construct phylogenic dendrogram or phylogram of closely related samples. Results indicate that the genetic distance between two samples of P. hyrcanicum originated from Sari and Goharbaran was considered to be short (0.622) and their RAPD and ISSR banding patterns were quite similar to each other also there is a close relationship between these two samples of Polygonum with P. avicular (0.848). The species P. hydropiper is not only far from two samples of P. hyrcanicum but also, has different patterns of RAPD and especially ISSR profiles compared to other samples. It is also interesting that P. hyrcanicum , gathered from Veresk, has no close relationship with other pairs of P. hyrcanicum . More chemotaxonomic investigation is needed to support the phylogenetic classification trees. Key words : Polygonum, Polygonaceae, molecular markers, P. hyrcanicum, P. persicaria, P. avicular, P.hydropiper

Method for remediation of phosphorus-contaminated soil

Abstract: The invention relates to a bioremediation method for phosphorus contamination, in particular to a method for remediation of phosphorus-contaminated soil, which comprises the following steps of: growing polygonum hydropiper in the phosphorus-contaminated soil; directly transplanting the polygonum hydropiper or directly sowing seeds of the polygonum hydropiper for growth; and regularly harvesting the overground part of the planted polygonum hydropiper. By utilizing the phosphorus enriching characteristic of the polygonum hydropiper, the polygonum hydropiper is grown in the soil, absorbs the phosphorus accumulated in the soil, and transfers most of phosphorus to the overground part so as to fulfill the aim of remediation of the phosphorus-contaminated soil. The polygonum hydropiper has vigorous vitality, is easy to survive, and does not need special management in the growing process, so the cost is low, and the operability is high. Meanwhile, the polygonum hydropiper has traditional Chinese medicinal effect of treating acute filthy disease stomachache, vomiting and diarrhea and spasm of calf, diarrhea, dysentery, rheumatism, dermatophytosis, carbuncle, mange, traumatic injury and the like, so the resource can be fully and reasonably utilized and is multipurpose.

Effect of Polygonum Multiflorum Thumb on learning and memory capacity in mice with AD

Abstract: Objective:To study the effect of Polygonum Multiflorum Thumb on the learning and memory capacity of mice with AD and its possible mechanism.Methods:weobserved the effect of Polygonum Multiflorum Thumb on the learning and memory capacity of mice with AD through Y-maze and CA1 area of hippocampal neurons by morphology.Results:Polygonum Multiflorum Thumb treatment group showed significant differences in the learning,memory capacity and the CA1 area cell number of hippocampal neurons,compared with morphine withdrawal group.Conclusion:Polygonum Multiflorum Thumb protects the learning and memory capacity of mice with AD,which may be related to the inhibition of the apoptosis of hippocampus.

Development and characterization of microsatellite markers for Polygonum cespitosum (Polygonaceae).

Abstract: Premise of the study: We isolated and characterized microsatellite markers in Polygonum cespitosum Blume, an herbaceous annual plant species introduced into North America from Asia that has recently become invasive. METHODS: and Results: A total of 12 polymorphic and 3 monomorphic loci were screened in 1-2 individuals from each of 20 populations from the introduced and native range, for a total of 24 samples. The number of alleles per locus in the polymorphic loci ranged from 3 to 9, and expected heterozygosity ranged from 0.156 to 0.838. CONCLUSIONS: These new loci will provide tools for examining genetic relatedness among introduced and native populations of this and other related species.

Correlation analysis of drug-induced hepatitis caused by Polygonum multiflorum Thunb

Abstract: To discuss the treatment and the related factors of drug-induced hepatitis caused by Polygonum multiflorum Thunb.Methods 26 cases with drug-induced hepatitis which caused by taking Polygonum multiflorum Thunb were analyzed.Results All patients were healed by the related liver-protected treatment.It taken 28～58 d for patients to recover.Conclusion The cause of Polygonum multiflorum Thunb leading to hepatitis is still unclear,which has a certain relationship with the allergic constitution of the patient itself,and it has a great significance in monitoring the number of transaminase during the diagnosis.

Cosmetic composition containing polygonum multiflorum extracts, jujube extracts and hibiscus manihot extracts for moisturizing skin

Abstract: PURPOSE: A cosmetic composition containing Polygonum multiflorum extract and Hibiscus manihot extract is provided to ensure antioxidation and to improve skin moisturizing. CONSTITUTION: A cosmetic composition for enhancing skin moisturizing contains Polygonum multiflorum extract, jujube extract, and Hibiscus manihot extract as an active ingredient. A cosmetic composition for promoting sirt-1 protein contains Polygonum multiflorum extract, jujube extract, and Hibiscus manihot extract as an active ingredient. The extracts are contained in each 0.01-20.0 weight%. The cosmetic composition is manufactured in the form of lotion, essence, lotion, cream, pack, gel, ointment, patch, or spray.