Showing papers on "Polygonum published in 2020"

Bioaugmentation-assisted phytoremediation of manganese and cadmium co-contaminated soil by Polygonaceae plants (Polygonum hydropiper L. and Polygonum lapathifolium L.) and Enterobacter sp. FM-1

Abstract: Polygonum hydropiper L. and Polygonum lapathifolium L. are two of the well-known indigenous Mn-hyperaccumulators. Enterobacter sp. FM-1 is a plant growth-promoting bacterium (PGPB) that we found in our previous study. We intended to develop a novel strategy to improve Mn and Cd co-contaminated soil phytoremediation by using a Mn and Cd-resistant bacterium for soil bioaugmentation. We carried out this study to investigate the effects of different Enterobacter sp. FM-1 inoculation concentrations (0, 5.0 × 105, 1.0 × 106 and 1.4 × 106 CFU g−1 soil) on the phytoremediation of Mn and Cd by Polygonum hydropiper L. and Polygonum lapathifolium L. in two types of Mn-Cd co-contaminated soil. In both soils, inoculation with Enterobacter sp. FM-1 promoted the growth of both plants. Moreover, inoculation with Enterobacter sp. FM-1 (≥1.0 × 106 CFU g−1 soil) significantly increased soil Mn and Cd bioavailability and decreased the soil pH. Therefore, inoculation with Enterobacter sp. FM-1 (1.4 × 106 CFU g−1 soil) improved Mn and Cd accumulation in both plants. Polygonum hydropiper L. presented excellent Mn accumulation in both soils. Additionally, both plants exhibited strong translocation and excellent phytoextraction and bioaccumulation abilities for Mn and Cd. Our findings indicated that Enterobacter sp. FM-1 is a potent bioaugmentation agent that facilitates Mn and Cd phytoextraction in Polygonum hydropiper L. and Polygonum lapathifolium L.

Stilbenes with Potent Protein Tyrosine Phosphatase-1B Inhibitory Activity from the Roots of Polygonum multiflorum

Abstract: Seven new stilbene glycosides including three dimers (1-3) and four monomers (4-7) were isolated from the roots of Polygonum multiflorum along with nine previously identified stilbenes (8-16). In addition, two deglucosylated stilbenes, 2a and 3a, were also obtained as new dimeric stilbenes. The structures of the purified phytochemicals were elucidated by interpreting their spectroscopic data (NMR, HRMS, and ECD). To the best of our knowledge, this represents the first isolation of a phenylpropanoid (C6-C3) substituted with a stilbene unit (7) from the Polygonaceae family. In an in vitro enzyme assay with human recombinant protein tyrosine phosphatase-1B (PTP1B), compounds 2-5 showed weak PTP1B inhibition with an IC50 value range of 27.4-37.6 μM, while three deglucosylated stilbenes 2a, 3a, and 8a exhibited IC50 values of 2.1, 1.9, and 12.1 μM, respectively. The inhibition modes and binding mechanism of selected inhibitors (2a and 3a) were investigated using kinetic methods and molecular docking simulations.

Leaves of Invasive Plants-Japanese, Bohemian and Giant Knotweed-The Promising New Source of Flavan-3-ols and Proanthocyanidins

Abstract: This is the first report on identification of all B-type proanthocyanidins from monomers to decamers (monomers-flavan-3-ols, dimers, trimers, tetramers, pentamers, hexamers, heptamers, octamers, nonamers, and decamers) and some of their gallates in leaves of Japanese knotweed (Fallopia japonica Houtt.), giant knotweed (Fallopia sachalinensis F. Schmidt) and Bohemian knotweed (Fallopia × bohemica (Chrtek & Chrtkova) J.P. Bailey). Flavan-3-ols and proanthocyanidins were investigated using high performance thin-layer chromatography (HPTLC) coupled to densitometry, image analysis, and mass spectrometry (HPTLC-MS/MS). All species contained (-)-epicatechin and procyanidin B2, while (+)-catechin was only detected in Bohemian and giant knotweed. (-)-Epicatechin gallate, procyanidin B1 and procyanidin C1 was only confirmed in giant knotweed. Leaves of all three knotweeds have the same chemical profiles of proanthocyanidins with respect to the degree of polymerization but differ with respect to gallates. Therefore, chromatographic fingerprint profiles of proanthocyanidins enabled differentiation among leaves of studied knotweeds, and between Japanese knotweed leaves and rhizomes. Leaves of all three species proved to be a rich source of proanthocyanidins (based on the total peak areas), with the highest content in giant and the lowest in Japanese knotweed. The contents of monomers in Japanese, Bohemian and giant knotweed were 0.84 kg/t of dry weight (DW), 1.39 kg/t DW, 2.36 kg/t, respectively, while the contents of dimers were 0.99 kg/t DW, 1.40 kg/t, 2.06 kg/t, respectively. Giant knotweed leaves showed the highest variety of gallates (dimer gallates, dimer digallates, trimer gallates, tetramer gallates, pentamer gallates, and hexamer gallates), while only monomer gallates and dimer gallates were confirmed in Japanese knotweed and monomer gallates, dimer gallates, and dimer digallates were detected in leaves of Bohemian knotweed. The profile of the Bohemian knotweed clearly showed the traits inherited from Japanese and giant knotweed from which it originated.

Flower, seed, and fruit development in three Tunisian species of Polygonum: Implications for their taxonomy and evolution of distyly in Polygonaceae.

Abstract: Polygonum is the largest genus of Polygonaceae and 5 species are reported in Tunisia. In order to characterized flower, seed, and fruit development in Polygonum, flower and fruit of Polygonium equisetiforme (var. graecum and peyerinhoffi), P. aviculare and P. maritimum, collected from Tunisia, were examined. Flowers are composed of five oblong tepals. P. equisetiforme and P. aviculare have whitish-pink distylous flowers with dimorphism of style, filament and anther height, pollen diameter and stigma size. In contrast, P. maritimum shows white homostylous flowers. The floral vasculature showed that the tepals are inserted in one whorl and their traces arise independently in 3+2 manner. The eight stamens are arranged in a 5+3 manner and the staminal bundles arise independently in the two whorls. The epidermis and endothecium cells width were higher in P. maritimum and the lowest endothecium width was observed in P. aviculare. Polygonum aviculare and P. equisetiforme showed circular pollen with shallow colpi and trilobite pollen shape with deep colpi, while P. maritimum rarely showed shallow colpi. The ovule is anatropous with basal placentation in P. equisetiforme and P. aviculare and apical placentation in P. maritimum. The young seed coat was formed by an endotesta with thick-walled cells, a mesotesta and exotesta with thin-walled cells and a tegmen composed of radially elongated cells. The fruits of the studied species are trigonous with ovate-lanceolate shape. In P. aviculare, the exocarp is thicker compared to the two other species, in P. equisetiforme, the mature exocarp consists of smaller rectangular cells with narrow cavities, and in P. maritimum showed a thinner exocarpIn conclusion, P. equisetiforme and P. aviculare are a typically distylous species from the morphological point of view and we discussed the significance of heterostyly in Polygonaceae. From this first morpho-anatomical study of Polygonum species in North Africa, we can conclude mainly that there is no significant difference between P. equisetiforme var. graecum and var. peyerinhoffi supporting a taxonomic grouping of these two varieties.

Differential Constituents in Roots, Stems and Leaves of Polygonum multiflorum Thunb. Screened by UPLC/ESI-Q-TOF-MS and Multivariate Statistical Analysis.

Abstract: The differential constituents in leaves, stems and roots of Polygonum multiflorum Thunb. were analyzed by ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS) and by multivariate statistical analysis. The established extraction and analysis method showed relative standard deviations (RSDs) for intra-day precision of less than 3.40%, for repeatability of less than 4.06% and for stability of less than 5.10%. Principal component analysis and orthogonal projections to latent structures discriminant analysis of the UPLC/ESI-Q-TOF-MS data showed good ability to classify the leaves, stems and roots of P. multiflorum Thunb. The differential constituents, such as stilbenes, polygoacetophenoside, flavonoids and anthraquinones, accounting for variations between the leaves, stems and roots, were filtered through the variable importance in projection values and were further identified by elemental composition analysis, mass fragmentation data and retention times of available standards. Differences between the chemical compositions in the leaves, stems and roots of P. multiflorum Thunb. were closely related to their various therapeutic effects. This UPLC/ESI-Q-TOF-MS-based analytical strategy could be further utilized to evaluate the overall quality of traditional Chinese medicines and their differences of chemical constituents in different parts of the plant and/or in the plants of different geographical locations.

Insecticidal effects of two medicinal plants Polygonum hydropiper L. and Abrus precatorius L. leaves against the rice weevil Sitophilus oryzae L. (Coleoptera: Curculionidae)

Abstract: During the study, the leaves of two medicinal plants, Polygonum hydropiper and Abrus precatorius were extracted with water, ethanol, methanol and petroleum ether solvents and tested against the rice weevil S. oryzae L. for insecticidal properties. Response varied with plant species. The mortality of adults increased with increasing dose concentrations from 1000 ppm to 5000 ppm each solution, with an exposure time of 72 hours. LC50 values calculated were found to be 15091.436 ppm with water, 5051.534 ppm with ethanol, 5111.063 ppm with methanol and 4305.348 ppm with petroleum ether extracts of P. hydropiper and 9687.292 ppm with water, 6263.849 ppm with ethanol, 4682.683 ppm with methanol and 3222.984 ppm with petroleum ether extracts of Abrus precatorius leaves corresponding their concentrations of the leaf extract solutions at 1000ppm, 2000 ppm, 3000 ppm, 4000 ppm and 5000 ppm respectively. The results of the study showed that methanol and petroleum ether extrcts of P. hydropiper and ethanol, methanol and petroleum ether extracts of A. precatorius showed good toxicity. It appeared that the leaf extracts had some insecticidal activities against S. oryzae adult. J. Biodivers. Conserv. Bioresour. Manag. 2019, 5(2): 107-114

Effects of Different Processed Products of Polygonum multiflorum on the Liver.

Abstract: Objective. Based on in vitro and in vivo experimental studies, the changes of the main components of Polygonum multiflorum and different processed products and their effects on hepatotoxicity were investigated. Methods. The components of different processed products of Polygonum multiflorum were determined by HPLC. The effects of processed products of different processing time periods on HepG2 cells were detected by using cell count kit-8 and the apoptosis method; the effects of different processed products on the mouse liver were detected by reverse transcription polymerase chain reaction and immunohistochemistry. Results. With the extension of processing time, the contents of various chemical components in Polygonum multiflorum increased, while the content of stilbene glucoside decreased. The serum of Polygonum multiflorum group and different steaming time groups had obvious inhibitory effect on HepG2 cells. For normal mice, the toxicity of raw Polygonum multiflorum and processed products at different processing time periods had certain toxicity to liver and gradually decreased with the prolongation of processing time. For mice in the liver injury group, the therapeutic effect of raw Polygonum multiflorum and processed products at different processing time periods was not obvious, but there is a trend of treatment. Conclusion. The content of the main components in Radix Polygonum multiflorum can be affected by processing time; stilbene glycoside may be the main component leading to liver injury. The degree of liver injury caused by Radix Polygonum multiflorum is negatively correlated with processing time.

Herb-Drug Interaction: Application of a UPLC-MS/MS Method to Determine the Effect of Polygonum capitatum Extract on the Tissue Distribution and Excretion of Levofloxacin in Rats

Abstract: Polygonum capitatum has unique curative effects on the urinary system In fact, many Polygonum capitatum-based preparations are currently used in the clinic In China, the combination of levofloxacin (LVFX) with a Chinese herbal preparation derived from Polygonum capitatum has been used for the clinical treatment of urinary system diseases, which can improve the curative effects and reduce the side effects of LVFX However, the herb-drug interaction (HDI) between these drugs has not been reported and the effect of Polygonum capitatum on the in vivo process of LVFX is unclear In this article, a sensitive ultraperformance liquid chromatography-tandem mass spectroscopy (UPLC-MS/MS) method was developed to evaluate the effects of the combined application of LVFX and the Polygonum capitatum extract on tissue distribution and excretion Thereafter, the method was validated for selectivity, accuracy, precision, linearity, lower limit of quantification (LLOQ), dilution integrity, recovery, and matrix effect Based on tissue distribution, LVFX could diffuse into all of the tested tissues, with significant differences in the content of each tissue between the coadministration group and single administration group At 48 h after the combination was orally administered, the urinary cumulative excretion of LVFX decreased from 2069% to 1184% while its fecal cumulative excretion decreased from 2608% to 1328% Our results suggest that a drug interaction exists between the two drugs in the process of distribution and excretion This study provides important experimental evidence for further studies on the clinical efficacy and mechanism of the Polygonum capitatum extract and LVFX

Evaluation of Alpha-Amylase Inhibitory, Antioxidant, and Antimicrobial Potential and Phytochemical Contents of Polygonum hydropiper L

Abstract: Polygonum hydropiper L. is a traditionally used medicinal plant. The present study was designed to explore the α-amylase inhibitory, antioxidant, and antimicrobial activities of Polygonum hydropiper L. Polarity-based solvent extracts (n-hexane, acetone, chloroform, methanol, ethanol, and water) of Polygonum hydropiper leaves and stem were used. Antioxidant activity was assessed by free radical scavenging assay (FRAP) and 2,2-diphenylpicrylhydrazyl (DPPH) free radical scavenging activity methods. Quantitative phytochemical analyses suggested that the stem of Polygonum hydropiper L. contains higher levels of bioactive compounds than its leaves (p < 0.05). The results suggested that stem-derived extracts of Polygonum hydropiper L. are more active against bacterial species, including two Gram-positive and three Gram-negative strains. Moreover, our results showed that the bioactive compounds of Polygonum hydropiper L. significantly inhibit α-amylase activity. Finally, we reported the polarity-based solvent extracts of Polygonum hydropiper L. and revealed that the stem, rather than leaves, has a high antioxidant potential as measured by FRAP and DPPH assay with IC50 values of 1.38 and 1.59 mg/mL, respectively. It may also be deducted from the data that the Polygonum hydropiper L. could be a significant candidate, which should be subjected to further isolation and characterization, to be used as an antidiabetic, antimicrobial and antioxidant resource in many industries, like food, pharmaceuticals and cosmetics.

The Influence of Polygonum Minus Huds on Bacteria to Acrylic Denture

Abstract: The user of acrylic partial denture more than 4,5% on Indonesia Country, expecially on West Borneo distrik. Used acrylic partial denture in mouth must be clean, because in the moulth many bacteria. Plaque is a collection of bacteria. Plaque causes of diseases on the teeth and moulth. Plak Cleaning plak using toothbrush and moulthwash. Polygonum minus Huds is the endemic plants in West Borneo. Contained of Polygonum minus Hud are phenolic compounds, flavonoids, alkaloids, steroids, terpenoids, tannins, saponins, and essential oils. It is can antibacterial in the form of Methanol Extract. the purpose of this study was to determine the antibacterial effects of infusion extracts on the collection of bacteria on acrylic denture in the mouth. This study is experimental laboratory research with a posttest only control group design. It was conducted by comparing the antibacterial of Polygonum minus Huds in infusa of 25%, 50%, and 75%. Antibacterial test uses dilution test Antimicrobial Susceptibility Testing (AST). Data analysis uses qualitative data analysis. The results of the study is infusa Polygonum minus Huds can reduce bakteri colony in acrylic denture. The higher the concentration of the infusa, the higher, the highter antibakteri effect

The genus Persicaria (Polygonaceae) in Turkey with a new taxon record

Abstract: Polygonaceae family mainly introduces itself with its stipules called ochrea. In Flora of Turkey, this family is indicated by eight genera that include Atraphaxis, Pteropyrum, Calligonum, Rheum, Oxyria, Polygonum, Rumex, Emex. This article emphasizes that the genus Polygonum and Persicaria are utterly different from each other. Full names and distributions of the species of Persicaria in Turkey are given in detail. A new Persicaria taxon is also reported from Turkey. A diagnostic key for Persicaria has been created for the first time. The taxonomic status of the Persicaria leblebicii which was recently given as a new species, has been discussed.

Confirmation and Characterization of the First Case of Acetolactate Synthase (ALS)-Inhibitor—Resistant Wild Buckwheat (Polygonum convolvulus L.) in the United States

Abstract: Wild buckwheat (Polygonum convolvulus L.) is a problem weed and ALS-inhibitors (e.g., chlorsulfuron) are commonly used for its management. Recently, a population of wild buckwheat (KSW-R) uncontrolled with ALS-inhibitors was found in a wheat field in Kansas, USA. The objectives of this research were to determine the level and mechanism of resistance to chlorsulfuron and cross resistance to other ALS-inhibitors in the KSW-R population. In response to chlorsulfuron rates ranging from 0 to 16x (x = 18 g ai/ha), the KSW-R wild buckwheat was found >100-fold more resistant compared to a known ALS-inhibitor susceptible (KSW-S) wild buckwheat. Also, >90% of KSW-R plants survived field recommended rates of sulfonylurea but not imidazolinone family of ALS-inhibitors. A portion of the ALS gene covering all previously reported mutations known to bestow resistance to ALS-inhibitors was sequenced from both KSW-R and KSW-S plants. The Pro-197-Ser substitution that confers resistance to the sulfonylurea herbicides was found in KSW-R plants. Our results support the evolution of high level of chlorsulfuron resistance as a result of a mutation in the ALS-gene in KSW-R buckwheat. This is the first case of resistance to any herbicides in wild buckwheat in the US.

Characteristics of endophytic bacteria from Polygonum hydropiper and their use in enhancing P-phytoextraction

Abstract: Endophytes are significant for their ecological role in plants and potential applications in phytoremediation. Endophytic bacteria with plant-growth-promoting (PGP) characteristics are isolated as inoculants to enhance phytoremediation efficiency; however, few attempts have been made to isolate bacteria from phosphorus (P)-accumulating plants and study their roles in P-phytoextraction. Endophytic bacteria of P-accumulating herb Polygonum hydropiper were isolated and identified. The PGP traits including indole-3-acetic acid (IAA), siderophores, phosphate-solubilization etc. were characterized. Some endophytes were selected as the inoculants for a pot experiment in high P soil to investigate their role in P-phytoextraction. Thirty-five isolates were tolerant of high P and classified into 10 taxonomic groups. The production of indole-3-acetic acid into IAA acid and siderophores was dependent on strain species. Tricalcium phosphate (TCP)-solubilizing activity of these strains was associated with a decrease of pH but increases of organic acids production, while phytin-solubilizing activity was associated with a decrease of pH but increases of acid and alkaline phosphatase activities. In the six inoculants, five strains promoted stem and leaf growth of Polygonum hydropiper except for SLs08. P. hydropiper with inoculation of SLr02, SLr15, SLs13 and SLs18 showed significantly higher root length and surface area than the control plants. The inoculation with SLr15, SLs13 and SLs18 significantly enhanced stem and leaf P accumulation of P. hydropiper. Endophytic bacteria with PGP traits exist in Polygonum hydropiper and strains SLr15, SLs13 and SLs18 greatly promoted plant growth and shoot P accumulation, offering the most potential as PGP inoculants for assisting P. hydropiper in P-phytoextraction.

Research on polyphenols extraction from Polygonum multiflorum Thunb. roots

Abstract: Summary Introduction: Polygonum multiflorum Thunb. is a herbal common plant in Asia, with many beneficial health effects for human because it contains many bioactive compounds which can prevent some diseases, for instance such as cardiovascular diseases, cancers, neurodegenerative diseases, etc. Objective: The purpose of this research is to point out the effects of extraction factors such as type of solvent, material/solvent ratio (w/v), solvent concentration (%, v/v), temperature (°C) and extraction time on the extraction yield of phenolic compounds from Polygonum multiflorum Thunb. roots, for instance, total polyphenol content (TPC) and antioxidant capacity (AC). Methods: The raw material consisting of Polygonum multiflorum Thunb root was extracted by the reflux maceration method. TPC and AC of received extract were evaluated by the Folin-Ciocalteu technique and DPPH method with Trolox as a standard agent. Results: The optimal conditions for the extraction process were acetone-water mixture (60%, v/v) as a solvent, material/solvent ratio of 1/40, extraction temperature of 50°C and extraction time of 90 minutes. The surface structure of material after extraction process changed insignificantly compared with the initial structure. Conclusion: The results showed that TPC and AC obtained the best values (38.60±0.56 mg GAE/g DW (dry weight) and 298.15±2.99 μmol TE/g DW, respectively) at optimal extraction conditions. In addition, some phenolic compounds were detected in the extract such as gallic acid, catechin and resveratrol.

Interaction of polyphenols extract from Polygonum multiflorum Thunb. roots with gelatin and toxicity of extract in mice.

Abstract: The roots of Polygonum multiflorum Thunb. (Vietnamese name: Ha-thu-o-do, HTOD) are used in processed form or the raw state in traditional Vietnamese medicine for many diseases and in extract form in the food industry. Some studies pointed out that HTOD extract had toxicity in humans. However, the toxicity of this herb plant currently remains unclear. In addition, this material contained a large amount of bioactive compounds, especially phenolic compounds. They have a strong antioxidant capacity and they can also interact with many different substrates such as protein, enzyme, lipid and carbohydrate. In this study, the received extracts from HTOD had the polyphenols concentrations of 415, 277, 208 and 166 (mg GAE.L-1), respectively. Besides, we only evaluated the gelatin-polyphenols interaction and the toxicity of HTOD extract in Swiss mice. The results show a strong gelatin-polyphenols interaction and no acute or subacute toxicity in mice. The polyphenols extract of HTOD at the concentration tested in this study is safe to use in food.

Antibacterial Mouthwash of Kesum Leaf (Polygonum Minus Huds) Essential Oil on Streptococcus Mutans and Sanguinis

Abstract: Polygonum Minus Huds methanol extract from several previous studies has antibacterial properties in bacteria in the mouth such as Streptococcus Mutans, Staphylococcus Aureus, Salmonella Typhi, Escherichia Coli, etc. The cause of gingivitis is Streptococcus Sanguinis and anaerobic bacteria in the subgingival, which is porphyromonas gingivalis. Polygonum Minus Huds has the potential to be an antibacterial mouthwash for bacteria that causes caries and gingivitis. This research aims to make a mouthwash based on the essential oil of Polygonum Minus Huds and determine its antibacterial properties in mouthwashes of 0.025% and 0.05% concentrations to find antibacterial activity on Streptococcus Mutans and Sanguinis. The research was conducted in June 2019 at the Chemistry Laboratory of Faculty of Mathematics and Natural Sciences, the University of Tanjungpura in Pontianak Lab and Health Polytechnique Pontianak Integrated Lab. Design Quasi-experimental research used post-test only control group design, mouthwash formulation with essential oil concentrations of 0.025% and 0.05%, positive control using non-alcoholic mouthwash, and negative control (Aquades). Statistical analysis was performed univariately. The results showed that the best mouthwash was 2.5 ml of Tween 80 and 2.5 ml of glycerin. The results showed the essential oil gargle of Kesum leaves (Polygonum Minus Huds) at concentrations of 0.05%, and 0.02%, which is the bacteria of Streptococcus Mutans and Streptococcus Sanguinis were not able to inhibit the growth of the two bacteria. Increasing the amount of concentration or making mouthwash in other forms is expected to increase its antibacterial effect.

Comparative Tissue Distribution of 6 Major Polyphenolic Compounds in Normal and Myocardial Ischemia Model Rats After Oral Administration of the Polygonum orientale L. Extract

Abstract: A simple, rapid, and selective ultra-performance liquid chromatography-mass spectrometry (MS)/MS method was established to investigate tissue distribution of 6 polyphenolic compounds of Polygonum o...

Composition for preventing and treating termites and preparation method thereof

Abstract: The invention provides a composition for preventing and treating termites and a preparation method thereof. The composition for preventing and treating termites is prepared from the following raw materials in parts by weight: 15 to 25 parts of melia azedarach oil, 15 to 25 parts of azadirachtin, 15 to 25 parts of toosendanin, 5 to 11 parts of herba asari extract, 4 to 8 parts of polygonum hydropiper extract, 4 to 8 parts of a biological additive, 6 to 10 parts of rhizoma pinelliae and 90 to 100 parts of soft water. An extraction process of the polygonum hydropiper extract comprises the following steps: air-drying the polygonum hydropiper and crushing the polygonum hydropiper by using a crusher; sieving the crushed raw material with a 90-100-mesh sieve, adding the powder into a glass container, adding 95% ethanol of which the mass is 4-5 times of that of the polygonum hydropiper powder, leaching the mixture for 24 hours and collecting a primary filtrate, adding 95% ethanol of which themass is 2-3 times of that of filter residues, leaching the filter residue for 24 hours and collecting a secondary filtrate, mixing the two filtrates, uniformly mixing the liquids, and carrying out standing treatment for 24 hours to obtain the polygonum hydropiper extract. The termite control composition prepared by the invention has the excellent characteristics of no toxicity, high efficiency, easy degradation, no residue and the like, and fully reflects the concept of environmental protection.

Method for raising chicken by using polygonum multiflorum

Abstract: The invention discloses a method for raising chickens by using polygonum multiflorum, and belongs to the field of breeding. The method comprises the following steps: (1) building of a chicken house; (2) selection of an ecological breeding land: feeding the chicken with feed in the morning and in the evening and feeding drinking water at intervals, wherein the feed comprises the following components in parts by weight: 15-30 parts of polygonum multiflorum leaves or vines of polygonum multiflorum, 30-50 parts of corn powder, 1-5 parts of cortex phellodendri leaves, 10-20 parts of rice bran and 20-30 parts of soybean powder; and the drinking water is a water decoction of the polygonum multiflorum leaves or polygonum multiflorum vines; and (3) slaughtering of ecological chickens. The method provided by the invention can provide balanced nutrition for the growth of live chickens, the quality of the chickens is improved, and the chicken is delicious and elastic, and has effects of replenishing vital essence and blood, and reinforcing the liver and kidney, and the breeding cost is saved.

Novel method for raising seedlings with Polygonatum multiflorum seeds

Abstract: The invention belongs to the technical field of artificial planting of medicinal plants, and particularly relates to a novel method for raising seedlings with Polygonatum multiflorum seeds. The novelmethod comprises the steps of sand storage of seeds, seedling raising with nutrient trays, grass control by covering and seedling emergence management. The novel method for raising seedlings with Polygonatum multiflorum seeds helps increase the growth speed of Polygonatum multiflorum seedlings, lower the management cost and meet the requirement for quick and efficient production of the polygonum multiflorum seedlings, and is suitable for large-scale production of Polygonum multiflorum seedlings.

Polygonum capitatum planting method

Abstract: The invention discloses a polygonum capitatum planting method. The method comprises the following steps: disinfecting seeds of polygonum capitatum to remove quilts; preparing a base fertilizer from polygonum capitatum waste residues, sawdust, fine soil, a zinc fertilizer and humic acid substances; laying the base fertilizer on a seedbed in a plastic greenhouse, and watering after laying is completed; from February to March, evenly mixing the processed polygonum capitatum seeds with the fine soil, and then evenly scattering the mixture on the seedbed; from March to April, opening a film of thegreenhouse, removing weeds, and watering; before transplanting, laying the base fertilizer in a field, then finely crushing the surface soil, and evenly mixing the compound fertilizer with the surfacesoil and then laying on the outermost layer of the field; after field preparation is completed, transplanting to the field; 10-20 days after transplanting, removing the weeds, applying the fertilize,and then carrying out conventional weeding and deinsectization every month; and harvesting in sunny days from August to November, drying in the sun and warehousing. The method disclosed by the invention has the characteristics of low medicine residue treatment and polygonum capitatum planting cost, good polygonum capitatum growth, high yield and good quality.

Composition for skin external application containing Polygonum multiflorum extract heightened anti-inflammatory activity

Abstract: The present invention relates to an anti-atopic composition for skin external application, which improves anti-inflammatory activity by extracting Fallopia multiflora roots with mineral hot spring water to ameliorate atopic dermatitis and improve treatment effects.