Showing papers on "Polygonum published in 2021"
TL;DR: In this article, the anti-Alzheimer's studies on crude extracts, essential oils and isolated compounds including β-sitostrol from Polygonum hydropiper L, motivated them to further studies against beta amylo...
Abstract: Our previous anti-Alzheimer's studies on crude extracts, essential oils and isolated compounds including β-sitostrol from Polygonum hydropiper L, motivated us for further studies against beta amylo...
10 citations
TL;DR: The results showed that the metabolic pattern of adventitious root samples was similar under different culture conditions; however, these samples could be discriminated from each other in pilot-scale bioreactors by Fourier transform infrared (FT-IR) spectroscopy.
Abstract: Bioreactor cultures have been used for biomass production and bioactive compounds accumulation in adventitious root cultures of medicinal plants. In this study, we evaluated the effects of different auxin types [indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA)] and concentrations (0.5, 1.0, 2.0 and 4.0 mg·L−1), Murashige and Skoog (MS) medium salt strength (0.25, 0.5, 0.75, 1.0, 1.5 and 2X), and sucrose concentrations (0, 1.5, 3, 5, 7 and 10%) on Polygonum multiflorum Thunb. adventitious root cultures in a 3-L balloon-type bubble bioreactor (BTBB). IBA (1, 2, and 4 mg·L−1) was more effective than NAA in promoting root growth. Additionally, low MS salt strength (0.25 and 0.5X MS) increased the accumulation of total phenolics and flavonoids but reduced biomass accumulation. Four weeks of culture in full-strength MS medium supplemented with 2 mg·L−1 IBA and 5% sucrose resulted in the highest root biomass [98.46 g·L−1 fresh weight (FW); 13.46 g·L−1 dry weight (DW)] and bioactive compounds accumulation (total phenolics compounds, 53.08 mg·g−1 DW; total flavonoids, 25.10 mg·g−1 DW). To determine whether metabolic fingerprinting of whole-cell extracts could be used to compare metabolic equivalence of P. multiflorum root samples, we treated adventitious roots with different culture conditions, and analyzed the treated adventitious roots and natural roots by Fourier transform infrared (FT-IR) spectroscopy. The results showed that the metabolic pattern of adventitious root samples was similar under different culture conditions; however, these samples could be discriminated from each other in pilot-scale bioreactors. Overall, our study provides useful information for industrial-scale cultivation of P. multiflorum adventitious roots. The optimum condition for bioreactor culture of Polygonum multiflorum is full-strength MS medium supplemented with 2 mg·L-1 IBA and 5% sucrose after 4 weeks of culture. FT-IR analysis can rapidly discriminate among root samples based on total metabolite equivalence.
9 citations
23 Mar 2021
TL;DR: In this article, two shading levels (50% and 70% shading levels) and one absolute control (0%) were studied under eight weeks and 16 weeks of exposures on Polygonum minus after two weeks.
Abstract: The growing demand for high value aromatic herb Polygonum minus-based products have increased in recent years, for its antioxidant, anticancer, antimicrobial, and anti-inflammatory potentials. Although few reports have indicated the chemical profiles and antioxidative effects of Polygonum minus, no study has been conducted to assess the benefits of micro-environmental manipulation (different shading levels) on the growth, leaf gas exchange and secondary metabolites in Polygonum minus. Therefore, two shading levels (50%:T2 and 70%:T3) and one absolute control (0%:T1) were studied under eight weeks and 16 weeks of exposures on Polygonum minus after two weeks. It was found that P. minus under T2 obtained the highest photosynthesis rate (14.892 µmol CO2 m−2 s−1), followed by T3 = T1. The increase in photosynthesis rate was contributed by the enhancement of the leaf pigments content (chlorophyll a and chlorophyll b). This was shown by the positive significant correlations observed between photosynthesis rate with chlorophyll a (r2 = 0.536; p ≤ 0.05) and chlorophyll b (r2 = 0.540; p ≤ 0.05). As the shading levels and time interval increased, the production of total anthocyanin content (TAC) and antioxidant properties of Ferric Reducing Antioxidant Power (FRAP) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) also increased. The total phenolic content (TPC) and total flavonoid content (TFC) were also significantly enhanced under T2 and T3. The current study suggested that P.minus induce the production of more leaf pigments and secondary metabolites as their special adaptation mechanism under low light condition. Although the biomass was affected under low light, the purpose of conducting the study to boost the bioactive properties in Polygonum minus has been fulfilled by 50% shading under 16 weeks’ exposure.
6 citations
TL;DR: Wang et al. as discussed by the authors found that 60% ethanol refined fraction (PMT-E) of polygonum multiflorum thunb at 50 μg mL−1, which contained two main bioactive compounds, 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside (TSG) and emodin-8-O, β-Dglucosaide (EG), could significantly increase the mean lifespan by 19.82%, delay the age-related decline of
Abstract: Polygonum multiflorum Thunb (PMT), as a traditional Chinese herbal medicine, has been widely used in the prevention and treatment of aging-related diseases, including Alzheimer's disease, Parkinson's disease, hyperlipidemia, atherosclerosis and inflammation. However, the effect of PMT on the lifespan and its molecular mechanisms are still unclear. Here we found that 60% ethanol refined fraction (PMT-E) of Polygonum multiflorum Thunb at 50 μg mL−1, which contained two main bioactive compounds, 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside (TSG) and emodin-8-O-β-D-glucoside (EG), could significantly increase the mean lifespan by 19.82%, delay the age-related decline of phenotypes, enhance stress resistance and reduce ROS accumulation in Caenorhabditis elegans. Moreover, we also found that the mitochondrial membrane potential (ΔΨ) and ATP content of worms treated with 50 μg mL−1 PMT-E were obviously improved. Further mechanistic studies revealed that DAF-16, SIR-2.1 and SKN-1 transcription factors were required for PMT-E-mediated lifespan extension. Finally, we found that PMT-E could significantly inhibit the toxicity induced by β-amyloid (Aβ) in Aβ transgenic worms. Altogether, these findings laid the foundation for the use of Polygonum multiflorum Thunb to treat aging and age-related diseases.
6 citations
TL;DR: In this paper, a fungus was identified as Candida glabrata using morphology, physiology, biochemistry, and molecular biology testing methods, and the results showed that the minimum inhibitory concentrations (MIC80) of Cortex phellodendri was 0.25μg/μL.
5 citations
TL;DR: In this article, the effects of sulfate supply on the growth, manganese (Mn) accumulation efficiency and Mn stress alleviation mechanisms of polygonum lapathifolium Linn.
5 citations
TL;DR: This study attempts to identify the members of Indian Polygonaceae based on permanent and stable foliar morphological characters by identifying four distinct principal component analysis (PCA) clusters separating theMembers of Persicaria, Polygonum, Rumex and Fagopyrum.
5 citations
4 citations
20 Feb 2021
TL;DR: In this article, the authors used HPTLC coupled with densitometry, image analysis and mass spectrometry (HPTLC-MS/MS) to detect proanthocyanidins in Japanese, Bohemian and giant knotweed rhizomes.
Abstract: Flavan-3-ols and proanthocyanidins of invasive alien plants Japanese knotweed (Fallopia japonica Houtt.), giant knotweed (Fallopia sachalinensis F. Schmidt) and Bohemian knotweed (Fallopia × bohemica (Chrtek & Chrtkova) J.P. Bailey) were investigated using high performance thin-layer chromatography (HPTLC) coupled to densitometry, image analysis and mass spectrometry (HPTLC-MS/MS). (+)-Catechin, (-)-epicatechin, (-)-epicatechin gallate and procyanidin B2 were found in rhizomes of these three species, and for the first time in Bohemian knotweed. (-)-Epicatechin gallate, procyanidin B1, procyanidin B2 and procyanidin C1 were found in giant knotweed rhizomes for the first time. Rhizomes of Bohemian and giant knotweed have the same chemical profiles of proanthocyanidins with respect to the degree of polymerization and with respect to gallates. Japanese and Bohemian knotweed have equal chromatographic fingerprint profiles with the additional peak not present in giant knotweed. Within the individual species giant knotweed rhizomes and leaves have the most similar fingerprints, while the fingerprints of Japanese and Bohemian knotweed rhizomes have additional peaks not found in leaves. Rhizomes of all three species proved to be a rich source of proanthocyanidins, with the highest content in Japanese and the lowest in Bohemian knotweed (based on the total peak areas). The contents of monomers in Japanese, Bohemian and giant knotweed rhizomes were 2.99 kg/t of dry mass (DM), 1.52 kg/t DM, 2.36 kg/t DM, respectively, while the contents of dimers were 2.81 kg/t DM, 1.09 kg/t DM, 2.17 kg/t DM, respectively. All B-type proanthocyanidins from monomers to decamers (monomers-flavan-3-ols, dimers, trimers, tetramers, pentamers, hexamers, heptamers, octamers, nonamers and decamers) and some of their gallates (monomer gallates, dimer gallates, dimer digallates, trimer gallates, tetramer gallates, pentamer gallates and hexamer gallates) were identified in rhizomes of Bohemian knotweed and giant knotweed. Pentamer gallates, hexamers, hexamer gallates, nonamers and decamers were identified for the first time in this study in Bohemian and giant knotweed rhizomes.
3 citations
01 Jan 2021
TL;DR: The experimental results showed that the anti-inflammatory effect of the total flavonoids in the high-dose group was basically the same as that in the low- dose group, and it was suggested that Quercetin and Kaempferol may be theAnti-inflammatory flavonoid in Polygonum capitatum.
Abstract: To observe the inhibitory effect of Polygonum capitatum extract on auricle swelling induced by xylene in mice, and explore the mechanism of anti-inflammatory action with the method of network pharmacology. Extract the total flavonoids, sugars and tannins from Polygonum capitatum, and act on xylene induced auricular inflammation in mice. Use 8mm hole punch to lay down the ear piece and measure the quality of the ear piece. Through consulting CNKI, Wanfang, Vip and other databases, the chemical constituents related to Polygonum capitatum were searched. Screening the active chemical constituents of Polygonum capitatum by ADME parameters. Find a target with the active chemical composition and establish a target data set; Protein interaction network (PPI) was used to construct the protein interaction network of target and inflammatory target of Polygonum capitatum. Cytoscape software was used to construct the complex network diagram of “component-target-pathway-disease”; DAVID was used for GO functional enrichment analysis and KEGG pathway enrichment analysis. Result: The experimental results of auricle inflammation induced by xylene in mice showed that the high dose group of total flavones, the low dose group of total flavones and the low dose group of tannin had inhibitory effects on auricle inflammation induced by minor xylene in mice. There were 37 reported compounds, 6 active compounds and 2 flavonoids compounds. A total of 268 targets were retrieved from 6 active compounds, and 41 potential targets were most closely related to the anti-inflammatory mechanism of the Polygonum capitatum.Through the biological function pathway enrichment and KEGG pathway enrichment analysis, 20 biological processes and 76 signal pathways were screened. Conclusion: The anti-inflammatory components of the Polygonum capitatum predicted by the network pharmacology and the experimental results showed that the anti-inflammatory effect of the total flavonoids in the high-dose group was basically the same as that in the low-dose group. It is suggested that Quercetin and Kaempferol may be the anti-inflammatory flavonoids in Polygonum capitatum.
1 citations
Patent•
07 Apr 2021
TL;DR: In this paper, an extract of polygonum minus is provided in an amount effective to reduce oxidative damage, which includes quercetin-3-glucuronide and quercitrin.
Abstract: The present invention relates to a composition containing an extract from the Polygonum minus for cognition and cosmetic purposes. The extract of Polygonum minus includes quercetin-3-glucuronide and quercitrin. Extract of Polygonum minus is provided in an amount effective to reduce oxidative damage. A process for isolation of an active extract from Polygonum minus includes the steps of a) subjecting the Polygonum minus including aerial parts of the plant to solvent extraction preferably with a material solvent ratio of 1:10-20 by percolation at a temperature range of 70-105° C. preferably 80° C. to obtain the extract; and b) filtering the extract obtained from step (a) followed by concentration and drying involving freeze drying, spray drying or vacuum belt drying to obtain therefrom the dry extract powder.
Patent•
05 Feb 2021
TL;DR: In this paper, a direct seeding mixture of the polygonum viviparum bulbil is used for seeding, and the problem of low emergence rate after seeding of polygonal veinil can be effectively solved.
Abstract: The invention belongs to the technical field of traditional Chinese medicinal material planting, and particularly relates to a polygonum viviparum growth regulator, a direct seeding mixture of polygonum viviparum bulbil and application of the polygonum viviparum growth regulator and the direct seeding mixture of the polygonum viviparum bulbil in polygonum viviparum planting. Specifically, the polygonum viviparum growth regulator comprises two or three of a rooting agent, gibberellin and brassinolide. The direct seeding mixture of the polygonum viviparum bulbil comprises the polygonum viviparumbulbil treated by the growth regulator and a water-retaining agent. When the direct seeding mixture of the polygonum viviparum bulbil is used for seeding, the problem of low emergence rate after seeding of the polygonum viviparum bulbil can be effectively solved.