Polygonum

About: Polygonum is a research topic. Over the lifetime, 1230 publications have been published within this topic receiving 12765 citations.

Polygoacetophenoside, A New Acetophenone Glucoside from Polygonum multiflorum1.

Abstract: Polygoacetophenoside ( 3), a new acetophenone glucoside, was isolated from POLYGONUM MULTIFLORUM (Polygonaceae), together with quercetin 3- O-galactoside ( 1) and arabinoside ( 2). The structure of the new glucoside was deduced to be 2,3,4,6-tetrahy-droxyacetophenone 3- O-beta- D-glucoside ( 3) by its chemical and spectral data.

Effects of extract of Polygonum multiflorum on cell cycle arrest and apoptosis of human liver cell line L02.

Abstract: Objective To analyze the chemical constituents of Polygonum multiflorum extract which may cause human liver cell damage and to explore the mechanism. Methods Raw and processed Polygonum multiflorum were extracted by 70% ethanol, then raw and processed Polygonum multiflorum water-eluted material (RW and PW), 50% ethanol-eluted material (R50 and P50) and 95% ethanol-eluted material (R95 and P95) were obtained by absorbing through AB-8 macroporous resin, followed by water, 50% ethanol and 95% ethanol elution in order. The water extracts of raw and processed Polygonum multiflorum (RWE or PWE) were obtained by boiling them in water as usual. Normal human liver L02 cells were treated by different concentrations of eluted Polygonum multiflorum materials for different time, and the cell growth inhibition of each group was determined by methylthiazolyldiphenyl-tetrazolium bromide method. The chemical constituents which had a significant cytotoxicity to L02 cells were analyzed by high-performance liquid chromatography (HPLC). Morphological changes of L02 cells were observed by Giemsa staining and cell cycle distribution was observed by flow cytometry. Results It was found that 95% ethanol-eluted extracts of raw and processed Polygonum multiflorum showed significant growth inhibition on normal human liver L02 cells, while the other components showed no significant inhibition on cell growth. HPLC analysis showed that the main component in 95% ethanol-eluted extract of raw and processed Polygonum multiflorum was emodin at content of (18.53+/-2.96)% and (10.28+/-1.34)% respectively. Cell cycle analysis showed that 95% ethanol-eluted material of Polygonum multiflorum and emodin had a similar significant effect of S phase arrest and all could induce L02 cell apoptosis. Conclusion The main part of Polygonum multiflorum causing liver cell damage is the 95% ethanol-eluted extract, and emodin is one of the important chemical constituents leading to liver cell damage.

Biotransformation of 4-Hydroxybenzen Derivatives by Hairy Root Cultures of Polygonum multiflorum Thunb.

Abstract: The biotransformation of four 4-hydroxybenzen derivatives (1,4-benzenediol (compound 1), 4-hydroxybenzaldehyde (compound 2), 4-hydroxybenzyl alcohol (compound 3) and 4-hydroxybenzoic acid (compound 4)) by the hairy root cultures of Polygonum multiflorum Thunb. as a new biocatalyst was investigated. It was found that the substrates were transformed to their corresponding glucosides, 4-hydroxyphenyl β-D-glucopyranoside (arbutin, compound 1a), 4-hydroxymethylphenyl β-D-glucopyranoside (gastrodin, compounds 2a, 3a) and 4-carboxyphenyl α-D-glucopyranoside (compound 4a), respectively. In the meantime, the hairy roots of P. multiflorum were able to stereoselectively and regioselectively glucosylate phenolic hydroxyl groups of compounds 1–4, but the cultures could not glucosylate the aldehyde group of compound 2 or the benzylic hydroxyl group of compound 3, and no glucosyl esterification of carboxyl groups of compound 4 was detected. On the other hand, the result also showed that the hairy roots of P. multiflorum were able to reduce the 4-hydroxybenzaldehyde to its corresponding alcohol. This is the first report that substrate 4 has been converted into its α-D-glucopyranoside by a plant biotransformation system.

Polyphenols from aerial parts of Polygonum bellardii and their biological activities.

Abstract: Context: Polygonum species have been used in the treatment of several types of inflammatory disorders and cancer. Nevertheless, there are no reports related to the anti-inflammatory and anti-proliferative activities of Polygonum bellardii All. (Polygonaceae).Objective: This study investigated the chemical composition of the methanol extract of P. bellardii. The anti-inflammatory and cytotoxic activities of methanol, n-butanol, ethyl acetate extracts and isolated polyphenols were determined.Materials and methods: The chemical structure of the isolated compounds was elucidated using different spectral techniques. MTT assay was used to evaluate the anti-proliferative activity in HeLa, MCF-7 and HepG-2 cells. Inhibition of 5-lipoxygenase (5-LOX) activity and prostaglandin E2 (PGE2) production in stimulated HepG-2 cells were used to assess the anti-inflammatory activity.Results: The present study resulted in isolation of five compounds (new for the species). They were identified as gallic acid (1), que...

Antimicrobial activity of some water plants from the northeastern Anatolian region of Turkey.

Abstract: The antimicrobial activity of methanol and acetone extracts of Butomus umbellatus, Polygonum amphibium, and two species of the genus Sparganium (S. erectum and S. emersum)against three Gram-positive, five Gram-negative bacteria and one fungus was assessed by the disk diffusion method. The microorganisms used were Staphylococcus aureusATCC-29740, Escherichia coli ATCC-25922, Pseudomonas aeruginosa ATCC-15442, Salmonella typhi NCTC-9394, Klebsiella pneumoniae NCTC-5046, Proteus vulgaris ATCC-7829, Bacillus subtilis ATCC-6633, Corynebacterium diphteriae RSHM-633 and Candida albicans ATCC-10231. Methanol extracts of the plants did not exhibit any inhibitory activity against any of the microorganisms, while the acetone extracts of the all tested plants only showed significant activity against Bacillus subtilis, with inhibition zones and minimal inhibitory concentration values in the 7-16 mm and 0.49-12.50 mg/mL ranges, respectively.