Topic
Polysaccharide Hydrolase
About: Polysaccharide Hydrolase is a research topic. Over the lifetime, 28 publications have been published within this topic receiving 415 citations.
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TL;DR: Polysaccharide hydrolase activity was assayed in a group of 28 selected Rhizopus strains and the production of lichenases, mannanases, cellulases, xylanases, amylases and pullulanases was demonstrated using the gel-testing method during growth of the strains on suitably meshed poly Saccharide gels.
Abstract: Polysaccharide hydrolase activity was assayed in a group of 28 selectedRhizopus strains. The production of lichenases, mannanases, cellulases, xylanases, amylases and pullulanases was demonstrated using the gel-testing method during growth of the strains on suitably meshed polysaccharide gels.
11 citations
TL;DR: Results indicate that hadal-zone amphipod can be good candidates as the new enzyme resource in the hadal region deeper than 7,000 m, and shows close relationships among amphipods, despite the geographic distance between the localities.
Abstract: Hirondellea species are common inhabitants in the hadal region deeper than 7,000 m. We found that Hirondellea gigas thrived in the Challenger Deep possessed polysaccharide hydrolases as digestive e...
11 citations
Patent•
14 Jun 1991
TL;DR: In this paper, the effect of polysaccharide hydrolases was investigated for textile treatment with cellulase and desizing of textile with α-amylase, and the results showed that the effect was increased by the addition of ethoxylated C12-C20 alcohol or alkyl-phenol with 10-100 ethoxy groups.
Abstract: Addition of ethoxylated C12-C20 alcohol or alkyl-phenol with 10-100 ethoxy groups increases the effect of polysaccharide hydrolases. Practical applications include textile treatment with cellulase and desizing of textile with α-amylase.
9 citations
TL;DR: This was the first report on the synergy between LPMOs and β-1,3-xylanase, and the strategy for enzyme self-immobilization was simple, timesaving, and efficient, which might have great potentials in algae biomass hydrolysis.
Abstract: Lytic polysaccharide monooxygenases (LPMOs) with synergistic effect on polysaccharide hydrolase represent a revolution in biotechnology, which may accelerate the conversion of biomass to the second-generation biofuels. Discovering more hydrolases that have synergism with LPMOs will considerably expand the knowledge and application of biomass degradation. The LPMOs named CgAA9 were verified to exhibit 1.52-fold synergism when incubated with β-1,3-xylanase at a molar ratio of 3:1. The ion chromatography results proved that CgAA9 did not alter the endogenous hydrolysis mode of β-1,3-xylanase. Meanwhile, to decrease the operational cost of enzymes, a novel strategy for immobilizing LPMOs and β-1,3-xylanases based on the biomimetic silica nanoparticles was developed. It enabled preparation of immobilized enzymes directly from the cell lysate. The immobilization efficiency and activity recovery reached 84.6 and 81.4%. They showed excellent reusability for 12 cycles by retaining 68% of initial activity. The optimum temperature for both free and immobilized biocatalyst were 40 and 37 °C, indicating they were ideal candidates for typical simultaneous saccharification and fermentation (SSF) in ethanol production from algea biomass. This was the first report on the synergy between LPMOs and β-1,3-xylanase, and the strategy for enzyme self-immobilization was simple, timesaving, and efficient, which might have great potentials in algae biomass hydrolysis. • The lytic polysaccharide monooxygenases (LPMOs) from Chaetomium globosum were firstly verified to boost the hydrolysis of β-1,3-xylanases for β-1,3-xylan.
• A novel strategy for simple preparation of SpyCather-modifed silica nanopartilcles and intelligent immobilization of target enzymes from the cell lysate was proposed.
• The immobilized LPMOs and β-1,3-xylanases could be reasonable alternatives for typical simultaneous saccharification and fermentation (SSF) in manipulation of algae biomass.
9 citations
Patent•
14 Jun 1991TL;DR: The effect of polysaccharide hydrolase is increased by incorporation of a water-soluble polymer of one or more monomers selected from the group of vinyl pyrrolidone, vinyl alcohol, acrylamide and soluble acrylates.
Abstract: The effect of a polysaccharide hydrolase is increased by incorporation of a water-soluble polymer of one or more monomers selected from the group of vinyl pyrrolidone, vinyl alcohol, acrylamide and soluble acrylates into the reaction system in dissolved form.
7 citations