Polysaccharide Hydrolase

Abstract: The use of several enzymes (tannase,polysaccharide hydrolase,polyphenol oxidase,β-glucosidase,etc.) and the application of immobilized enzyme technology in tea beverages were mainly reviewed and summarized together with the research status concerned,and the prospects discussed.

Abstract: PURPOSE:To obtain a drink, readily drinkable without crumbliness as well as sandiness and excellent in health from an in expensive raw material, by treating bean-curd refuse with a polysaccharide hydrolase and treating the resultant hydrolyzate in a homogenizer. CONSTITUTION:Bean-curd refuse left as strained lees in producing soybean milk and been curd, etc., is diluted with water, etc., to form a slurry. A polysaccharide hydrolase which is preferably pectinase, cellulase or hemicellulase is added to carry out enzymic treatment. The bean-curd refuse after the enzymic treatment is then treated under preferably 50-250kg/cm pressure in a homogenizer to afford the aimed drink containing dietary fibers and proteins.

Abstract: The invention belongs to the technical field of bioengineering and enzyme engineering, and discloses a polysaccharide hydrolase gene from pyrococcus horikoshii and application. A nucleotide sequence of the polysaccharide hydrolase gene Tk1765 is shown as SEQ ID NO:1. A coding sequence of the polysaccharide hydrolase gene Tk1765 is shown as SEQ ID NO:2. Polysaccharide hydrolase has the efficient cellulose gum and chitose double digest character. According to cellulose gum of the polysaccharide hydrolase, the optimum pH value is 10.0, and the optimum enzyme reaction temperature is 65 DEG C. According to hydrolysis chitose, the optimum pH value is 11.0, and the optimum enzyme reaction temperature is 80 DEG C. The hydrolase is heated for 4 h at the temperature being 100 DEG C, and the activity of the hydrolase can be kept to be 40% or above. A recombinant vector of the hydrolase is constructed and excessively expressed in pichia pastoris, a product of the hydrolase has the function of hydrolyzing maize straw, and the polysaccharide hydrolase and the coding gene thereof can be widely applied to cellulose and chitin degradation.

Abstract: Lytic polysaccharide monooxygenases (LPMOs) with synergistic effect on polysaccharide hydrolase represent a revolution in biotechnology, which may accelerate the conversion of biomass to the second-generation biofuels. Discovering more hydrolases that have synergism with LPMOs will considerably expand the knowledge and application of biomass degradation. The LPMOs named CgAA9 were verified to exhibit 1.52-fold synergism when incubated with β-1,3-xylanase at a molar ratio of 3:1. The ion chromatography results proved that CgAA9 did not alter the endogenous hydrolysis mode of β-1,3-xylanase. Meanwhile, to decrease the operational cost of enzymes, a novel strategy for immobilizing LPMOs and β-1,3-xylanases based on the biomimetic silica nanoparticles was developed. It enabled preparation of immobilized enzymes directly from the cell lysate. The immobilization efficiency and activity recovery reached 84.6 and 81.4%. They showed excellent reusability for 12 cycles by retaining 68% of initial activity. The optimum temperature for both free and immobilized biocatalyst were 40 and 37 °C, indicating they were ideal candidates for typical simultaneous saccharification and fermentation (SSF) in ethanol production from algea biomass. This was the first report on the synergy between LPMOs and β-1,3-xylanase, and the strategy for enzyme self-immobilization was simple, timesaving, and efficient, which might have great potentials in algae biomass hydrolysis. • The lytic polysaccharide monooxygenases (LPMOs) from Chaetomium globosum were firstly verified to boost the hydrolysis of β-1,3-xylanases for β-1,3-xylan. • A novel strategy for simple preparation of SpyCather-modifed silica nanopartilcles and intelligent immobilization of target enzymes from the cell lysate was proposed. • The immobilized LPMOs and β-1,3-xylanases could be reasonable alternatives for typical simultaneous saccharification and fermentation (SSF) in manipulation of algae biomass.

Abstract: PURPOSE:To produce a polysaccharide hydrolase by culturing a microbial strain belonging to Pseudomonas genus and capable of decomposing polysaccharides in a medium containing a specific sugar as an inducing substance. CONSTITUTION:A microbial strain belonging to Pseudomonas genus and capable of decomposing polysaccharides, e.g. Pseudomonas sp. PT-5 (FERM-BP 330) is cultured in a medium containing one or more sugars selected from di-, tri- or polysaccharide having beta-1, 3-xyloside bond, di-, tri- or polysaccharide having beta-1, 4-mannoside bond and tri- or polysaccharide having beta-1, 3- and beta-1, 4- galactoside bonds as an inducing substance, e.g. in a medium containing algae containing one or more kinds of the above saccharides as inducing substance. The objective polysaccharide hydrolase corresponding to the inducing substance used in the medium is separated from the culture liquid.