Topic
Polysaccharide Hydrolase
About: Polysaccharide Hydrolase is a research topic. Over the lifetime, 28 publications have been published within this topic receiving 415 citations.
Papers
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TL;DR: The ERT® technology clearly showed the possibility to discriminate endolytic and exolytic enzymatic mechanisms and the method allowed following the depolymerization of polysaccharides by PCE.
Abstract: Most of usual methods used to detect and quantify polysaccharide cleavage enzymes (PCE) are not adapted to high throughput. In this work, enzymatic activities of three enzymes, namely an endocellulase (EC 3.2.1.4), an exocellulase (EC 3.2.1.91) and an alginate lyase (EC 4.2.2.3) were characterized by classical Michaelian model (Km, Vmax). Enzymatic activities were then determined by the use of the Enzyme Ring Test® (ERT®) method which allowed following the depolymerization of polysaccharides by PCE. Kinetic parameters were determined and compared to the ones obtained by classical methods. Besides, the ERT® technology clearly showed the possibility to discriminate endolytic and exolytic enzymatic mechanisms.
Patent•
06 Jan 1987
TL;DR: In this paper, a Pseudomonas sp. PT-5 (FERM-BP 330) was cultured in a medium containing one or more sugars selected from di-, tri- or polysaccharide having beta-1, 3-xyloside bond, di, tri-, or poly-saccharides having tri-or poly-charide with beta- 1, 3 and beta 1, 4- galactoside bonds as an inducing substance.
Abstract: PURPOSE:To produce a polysaccharide hydrolase by culturing a microbial strain belonging to Pseudomonas genus and capable of decomposing polysaccharides in a medium containing a specific sugar as an inducing substance. CONSTITUTION:A microbial strain belonging to Pseudomonas genus and capable of decomposing polysaccharides, e.g. Pseudomonas sp. PT-5 (FERM-BP 330) is cultured in a medium containing one or more sugars selected from di-, tri- or polysaccharide having beta-1, 3-xyloside bond, di-, tri- or polysaccharide having beta-1, 4-mannoside bond and tri- or polysaccharide having beta-1, 3- and beta-1, 4- galactoside bonds as an inducing substance, e.g. in a medium containing algae containing one or more kinds of the above saccharides as inducing substance. The objective polysaccharide hydrolase corresponding to the inducing substance used in the medium is separated from the culture liquid.
Patent•
27 Jul 2011
TL;DR: In this paper, an isolated protein and a composition containing the same for enhancing polysaccharide hydrolase activity are provided to enhance hydrolysis ability and to reduce the use of hydrolases.
Abstract: PURPOSE: An isolated protein and a composition containing the same for enhancing polysaccharide hydrolase activity are provided to enhance hydrolysis ability and to reduce the use of hydrolase CONSTITUTION: An isolated protein enhances polysaccharide hydrolase activity and has polysaccharide binding ability The protein contains GH45(glycoside hydrolase 45) or pollen-allergen domain The polysaccharide hydrolase is cellulase, hemicellulase, alpha-amylase, glycoamylase, pectinase, xylanase, or cellobiase The GH45 domain or pollen allergen domain is isolated from expansin, expansin-like protein, or bacterial protein