Topic
Polytene chromosome
About: Polytene chromosome is a research topic. Over the lifetime, 2667 publications have been published within this topic receiving 88255 citations.
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TL;DR: The three-dimensional chromosome topography in an intact nucleus has been determined using fluorescently stained Drosophila polytene chromosomes, optical fluorescence microscopy and newly developed, generally applicable, cellular image reconstruction techniques.
Abstract: The three-dimensional chromosome topography in an intact nucleus has been determined using fluorescently stained Drosophila polytene chromosomes, optical fluorescence microscopy and newly developed, generally applicable, cellular image reconstruction techniques. The folding pattern is a complex mixture of parallel chromosomal segments and intertwined coils and shows extensive interaction of the chromosomes with the nuclear envelope.
422 citations
Journal Article•
TL;DR: Heterochromatin formation occurs at approximately the same stage at which nuclei first become transcriptionally competent, suggesting that the C1A9 antibody may serve as a useful marker for both structural and functional studies of the Drosophila nucleus.
417 citations
TL;DR: It is shown that co-expressed genes in C. elegans are clustered in groups of 2–5 along the chromosomes, suggesting that expression from a chromatin domain can extend over several genes.
Abstract: Chromosomes are divided into domains of open chromatin, where genes have the potential to be expressed, and domains of closed chromatin, where genes are not expressed1. Classic examples of open chromatin domains include ‘puffs’ on polytene chromosomes in Drosophila and extended loops from lampbrush chromosomes2,3. If multiple genes were typically expressed together from a single open chromatin domain, the position of co-expressed genes along the chromosomes would appear clustered. To investigate whether co-expressed genes are clustered, we examined the chromosomal positions of the genes expressed in muscle of Caenorhabditis elegans at the first larval stage. Here we show that co-expressed genes in C. elegans are clustered in groups of 2–5 along the chromosomes, suggesting that expression from a chromatin domain can extend over several genes. These observations reveal a higher-order organization of the structure of the genome, in which the order of genes along the chromosome is correlated with their expression in specific tissues.
415 citations
TL;DR: A simple, rapid method for retrieving cloned segments carrying a copy of the transposable element together with contiguous sequences corresponding to this allele and its application to the cloning of the DNA sequences of other genes in Drosophila is discussed.
407 citations
TL;DR: Individual segments of the chromosomal DNA in D. melanogaster were isolated, and the sequences they contain were analyzed for repetition and mapped within the polytene chromosomes, which are not compatible with tandem repetition models for the chromomere.
405 citations