Potato dextrose agar
About: Potato dextrose agar is a research topic. Over the lifetime, 3375 publications have been published within this topic receiving 37128 citations.
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TL;DR: The pathogen was identified as Alternaria dianthicola and further confirmed by the Agharkar Research Institute, Pune, India and was consistently reisolated from inoculated plants.
Abstract: Withania somnifera (L.) Dunal, a potential medicinal plant used for the treatment of nervous disorders, intestinal infection, leprosy, and cancer, is a perennial herb belonging to Solanaceae and distributed throughout the drier parts of India. Leaf blight disease of this plant generally occurs during March in various districts of South Bengal, India. At the initial stage of infection, symptoms appear as small, light brown spots, gradually becoming irregular, dark brown, concentrically zonate with a diffuse margin, frequently surrounded by light yellow haloes, conspicuous brownish concentric rings in the advance stage of infection. A species of Alternaria was isolated from the lesions. The pathogen was isolated on potato dextrose agar (PDA) media. On PDA, the fungus grew slowly with colonies reaching approximately 35 to 40 mm in diameter in 7 days when incubated at 30°C. Conidiophores arose singly or in groups, straight or flexous, cylindrical, septate, pale to olivaceous brown, as much as 155 μm long, 4 t...
TL;DR: The results indicated that AgNPs possess antifungal properties against these plant pathogens at various levels.
Abstract: This research is concerned with the fungicidal properties of nano-size silver colloidal solution used as an agent for antifungal treatment of various plant pathogens. We used WA-CV-WA13B, WA-AT-WB13R, and WA-PR-WB13R silver nanoparticles (AgNPs) at concentrations of 10, 25, 50, and 100 ppm. Eighteen different plant pathogenic fungi were treated with these AgNPs on potato dextrose agar (PDA), malt extract agar, and corn meal agar plates. We calculated fungal inhibition in order to evaluate the antifungal efficacy of silver nanoparticles against pathogens. The results indicated that AgNPs possess antifungal properties against these plant pathogens at various levels. Treatment with WA-CV-WB13R AgNPs resulted in maximum inhibition of most fungi. Results also showed that the most significant inhibition of plant pathogenic fungi was observed on PDA and 100 ppm of AgNPs.
TL;DR: Evidence is presented that oxalic acid is a pathogenicity determinant of the bean white mould fungus, Sclerotinia sclerotiorum, which is confirmed to be pathogenic when mutants were grown on nutrient media containing sodium succinate.
TL;DR: Analysis of in vitro antagonism against Colletotrichum gloeosporioides and Sclerotium rolfsii and culture filtrates indicated that the antifungal potential of the exponential culture Filtrate was mainly due to the presence of extracellular chitinase enzyme, whereas the antIfungal activity of the stationary culture filTrate involved the action of unknown thermostable antif fungus compound(s).
Abstract: Indigenous actinomycetes isolated from rhizosphere soils were assessed for in vitro antagonism against Colletotrichum gloeosporioides and Sclerotium rolfsii. A potent antagonist against both plant pathogenic fungi, designated SRA14, was selected and identified as Streptomyces hygroscopicus. The strain SRA14 highly produced extracellular chitinase and β-1,3-glucanase during the exponential and late exponential phases, respectively. Culture filtrates collected from the exponential and stationary phases inhibited the growth of both the fungi tested, indicating that growth suppression was due to extracellular antifungal metabolites present in culture filtrates. The percentage of growth inhibition by the stationary culture filtrate was significantly higher than that of exponential culture filtrate. Morphological changes such as hyphal swelling and abnormal shapes were observed in fungi grown on potato dextrose agar that contained the culture filtrates. However, the antifungal activity of exponential culture filtrates against both the experimental fungi was significantly reduced after boiling or treatment with proteinase K. There was no significant decrease in the percentage of fungal growth inhibition by the stationary culture filtrate that was treated as above. These data indicated that the antifungal potential of the exponential culture filtrate was mainly due to the presence of extracellular chitinase enzyme, whereas the antifungal activity of the stationary culture filtrate involved the action of unknown thermostable antifungal compound(s).
TL;DR: A strain of Bacillus subtilis which produces an antibiotic metabolite was also found to produce a volatile compound(s) which was antifungal to Rhizoctonia solani and Pythium ultimum.
Abstract: A strain of Bacillus subtilis which produces an antibiotic metabolite was also found to produce a volatile compound(s) which was antifungal to Rhizoctonia solani and Pythium ultimum. Growth of the fungi was severely impaired in the presence of the volatiles and physiological abnormalities of the hyphae were observed, including hyphal distortion and vacuolation. A range of media were tested for volatile production and potato dextrose agar (PDA) was found to be the most active. Temperature had a considerable effect on antifungal volatile activity with the greatest inhibition occurring at 30 degrees C. Addition of iron (III) chloride to Sabouraud's glucose agar (SGA) also enhanced the antifungal effect. The volatiles were found to be water soluble and remained active when trapped in SGA.
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