Prespliceosome

TL;DR: This work aims to provide a history of canine coronavirus infection in the context of central giant cell granuloma and aims to establish a cause-and-effect relationship between infection and disease progression.

TL;DR: It is suggested that base pairing between U1 and the 5' splice site is necessary but not sufficient for the splicing of mRNA precursors.

TL;DR: Recognition of the UACUAAC box relies, at least in part, on Watson-Crick base pairing with the yeast U2 analogue, and allele-specific suppression of biological and biochemical phenotypes was observed.

TL;DR: This work identifies a previously uncharacterized activity, U2AF, that is required for the U2 snRNP-branch point interaction and splicing complex formation and provides an explanation for the necessity of the 3' splice site region in U2SnRNP binding and, hence, the first step of splicing.

TL;DR: Electrophoretic separation of ribonucleoprotein particles in a nondenaturing gel was used to analyze the splicing of mRNA precursors and surprisingly, U4 snRNP was not part of the intron-containing complex, suggesting that U4/6 snR NP disassembles and assembles during splicing.