Showing papers on "Protein–protein interaction published in 1969"

Protein-Protein Interaction

Abstract: Publisher Summary This chapter discusses protein–protein interaction. The study of interactions between protein molecules is such a vast and diversified area that a comprehensive treatment of this subject will appear in another monograph in this series. There are two types of equilibria between macromolecules: (1) the interacting species are subunits which associate to form biologically active units and (2) interaction between distinct macromolecular entities occurs as a step in a biological process. There is also some indirect evidence from solution physical chemistry that some polymeric proteins owe their associative stability to hydrophobic bonding. The major problem that arises in an investigation of the formation of quarternary structure in solution, however, is the experimental recognition of alterations in the secondary and tertiary structure of the individual polypeptide chains as separate from the interaction between two such chains. Tobacco mosaic virus is a rodlike molecule consisting of 2130 protein subunits and a single RNA molecule occupying a hollow core inside the assembly of polypeptide chains. The protein component can be separated intact from the RNA and reversibly dissociated by urea, 67% acetic acid, or dilution.