Topic
Proteolytic enzymes
About: Proteolytic enzymes is a research topic. Over the lifetime, 23096 publications have been published within this topic receiving 835544 citations.
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TL;DR: This review will focus on the digestive proteases of the major blood-feeding nematodes - hookworms and the ruminant parasite, Haemonchus contortus - but also compares and contrasts these proteases with recent findings from schistosomes and malaria parasites.
188 citations
TL;DR: It is shown that proteolytic enzymes reduce the electrophoretic mobilities of human and chimpanzee erythrocytes, the decreases depending upon the specificity of the particular enzyme.
188 citations
TL;DR: An inhibitory substance was found to be a low molecular weight compound, heat stable, soluble in chloroform and resistant to proteolytic enzymes, which has potential applications for control of shrimp pathogenic vibrios in aquaculture systems.
187 citations
TL;DR: The results are in accord with the hypothesis that enzyme(s) having trypsin-like and carboxypeptidase B-like activity exist in the secretory granules of the β-cells and participate in the conversion of proinsulin to insulin.
187 citations
TL;DR: The technique affords a method for the direct measurement of fluxes across membranes of kidney cells, and there is more rapid accumulation of PAH and a higher rate of oxygen consumption in this preparation than in slices.
Abstract: A method for the separation of intact viable cells from rabbit renal cortex has been developed. Separation is achieved by treatment of the excised kidney with the proteolytic enzyme collagenase. The resultant suspension consists principally of short lengths of proximal tubules. Survival of the tissues is indicated by maintenance of oxygen consumption, PAH uptake, and electrolyte composition. Since, in contrast to renal cortical slices, the cells in the tubule suspension are bathed directly by a solution of uniform composition, there is more rapid accumulation of PAH and a higher rate of oxygen consumption in this preparation than in slices. The technique affords a method for the direct measurement of fluxes across membranes of kidney cells.
187 citations