Showing papers on "Protoplast published in 1969"
TL;DR: It was proved that the protoplasts prepared from mesophyll of Nicotiana tabacum are infected by tobacco mosaic virus, with the efficiency of infection exceeding that by mechanical inoculation of tobacco leaves.
Abstract: It was proved that the protoplasts prepared from mesophyll of Nicotiana tabacum are infected by tobacco mosaic virus. The infection occurred when purified tobacco mosaic virus particles were added to a protoplast suspension in the presence of poly-L-ornithine. The virus multiplied in these protoplasts to a level of 106 virus particles per infected protoplast during 24 hours of incubation. The efficiency of infection was remarkably high, exceeding that by mechanical inoculation of tobacco leaves.
173 citations
TL;DR: Isolated protoplasts from healthy tobacco mesophyll tissue were infected by brief contact with infectious RNA from tobacco mosaic virus, and a synchronous multiplication of TMV occurred, and the amount of intracellular virus reached a maximum after 22 hours.
172 citations
TL;DR: Hphal wall preparations of Apodachlya branchynema, Dictyuchus sterile, Pythium sp.
61 citations
TL;DR: It is found that urea and glycerol induced mechanical relaxation of protoplast membranes suggests that the mechanically important membrane component is protein, possibly in the form of a microfibrillar array.
42 citations
TL;DR: Yeast protoplasts are to be considered as completely devoid of the cell wall because in the replicas there were found prints in the non-etchable envelope which are complementary to the protoplast surface.
27 citations
TL;DR: Protoplast preparations made from Saccharomyces cerevisiae by prolonged treatment with snail digestive juice contained fibrils and chitinous bud-scar residues from the original cell wall.
Abstract: Protoplast preparations made from Saccharomyces cerevisiae by prolonged treatment with snail digestive juice contained fibrils and chitinous bud-scar residues from the original cell wall.
23 citations
TL;DR: Post-staining with phosphotungstic acid has been investigated using thin sections of isolated tomato fruit protoplast material and this selectivity of staining has been shown to be associated with pinocytic activity.
Abstract: Post-staining with phosphotungstic acid has been investigated using thin sections of isolated tomato fruit protoplast material. Certain areas of the plasmalemma and some cytoplasmic vesicles were found to stain intensely, and this selectivity of staining has been shown to be associated with pinocytic activity.
18 citations
TL;DR: The submicroscopic structure of growing and regenerating Schizosaccharomyces pombe protoplasts cultivated in solid and liquid medium was studied by means of ultrathin sectioning.
Abstract: The submicroscopic structure of growing and regeneratingSchizosaccharomyces pombe protoplasts cultivated in solid and liquid medium was studied by means of ultrathin sectioning. The protoplasts regenerate within 24 hours. Shortly before growth commences, rudiments of the new cell wall can be identified on the protoplast surface. Simultaneously, a large number of dictyosomes appears in the cytoplasm and decreases as synthesis of the new wall progresses. An increase occurs in the number of endoplasmic reticulum membranes some of which are arranged parallel with the cytoplasm membrane of the protoplast. Throughout the whole time of regeneration the protoplasts contain only one nucleus. The nucleo-cytoplasm ratio of growing and regenerating protoplasts is lower than in intact cells. The number of mitochondria falls at the outset of regeneration and does not rise again until towards the end.
16 citations