Topic
Protoplast
About: Protoplast is a research topic. Over the lifetime, 5474 publications have been published within this topic receiving 122468 citations.
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TL;DR: The results demonstrate that somatic hybrids derived from electrofusion of plant protoplasts were viable, regenerated to calli and exhibited organogenetic response by shoot differentiation.
51 citations
TL;DR: The cytological and preliminary genetical analyses suggest a synkaryon formation and integration of genetic markers from parental strains in protoplasts mixed to give intraspecific complementary combinations.
Abstract: SUMMARY: Protoplasts, prepared from auxotrophic strains of Saccharomyces cerevisiae, Schizosac-charomyces pombe and Hansenula wingei, were mixed to give intraspecific complementary combinations. Polyethylene glycol (PEG) was added to induce agglutination and fusion. Some of the fused products grew on the surface of solid minimal medium forming large vacuolated bodies. Others reverted to hybrid cells when embedded in solid minimal regeneration medium. The cytological and preliminary genetical analyses suggest a synkaryon formation and integration of genetic markers from parental strains. The frequency of intrageneric fusions assessed from the number of protoplasts growing on the surface of minimal agar was estimated to be 1 to 3%, while the frequency of hybrid colony formation in regeneration medium was less than 1%.
51 citations
TL;DR: It is demonstrated that by this newly developed thin-alginate-layer technique it is possible to regenerate shoots from leaf protoplasts of Nicotiana tabacum L. at very high efficiency and very rapidly, with the first shoots appearing within less than two weeks.
Abstract: Regeneration of plants from protoplasts is regarded a difficult and lengthy procedure which requires well developed skills on the side of the experimenter. Therefore, where alternative procedures for genetic engineering of plants are available, protoplast-based techniques are frequently avoided. Here, we demonstrate, that by our newly developed thin-alginate-layer technique it is possible to regenerate shoots from leaf protoplasts ofNicotiana tabacum L. at very high efficiency and very rapidly, with the first shoots appearing within less than two weeks. Root formation is induced on a third medium with first roots being found after only 10 more days of culture.
51 citations
TL;DR: Protoplasts were isolated from seedling roots of Medicago sativa and Trigonella foenumgraecum using a simple enzyme mixture and plantlets were recovered via somatic embryogenesis from protoplast derived tissues.
51 citations
TL;DR: It is demonstrated that protoplast fusion is the only possibility for obtaining hybrids between these two species, and no embryos, seeds, or plantlets were obtained, thus demonstrating the feasibility of producing sexual hybrids through in vivo and in vitro methods by cross pollination.
Abstract: After protoplast fusion somatic hybrid calli were obtained by complementation selection between an albino mutant of Datura innoxia and the wildtype of Atropa belladonna (Krumbiegel and Schieder, 1979. Planta 145, 371–375). In the present study experiments are described concerning leaf and shoot induction on several media supplemented with different combinations and concentrations of hormones. Except for fleshy leaves and embryos, no well-formed shoot could be obtained. However, under standard culture conditions after one and a half years, one line produced numerous green shoots, showing a reduced number of chromosomes from Atropa belladonna. The loss of some chromosomes decreased the degree of somatic incompatibility. The additional appearance of shoots with albino sectors, of total albino shoots, and of green shoots showing a different phenotype, demonstrated that the elimination of the chromosomes occurred not only once, but several times. At least one shoot nearly stable in chromosome content and green subline could be obtained possessing only 6 chromosomes of Atropa belladonna and the original chromosome number of Datura innoxia. Experiments were carried out to test the feasibility of producing sexual hybrids through in vivo and in vitro methods by cross pollination. However, no embryos, seeds, or plantlets were obtained, thus demonstrating that protoplast fusion is the only possibility for obtaining hybrids between these two species.
51 citations