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Protoplast
About: Protoplast is a research topic. Over the lifetime, 5474 publications have been published within this topic receiving 122468 citations.
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TL;DR: It was concluded that, during the two cell cycles after protoplast isolation, there is a frequency peak which is strikingly higher than during both mesophyll cell divisions in leaf tissues and furthercell divisions in vitro.
37 citations
TL;DR: Ch Chromosome observation revealed that all of the plants examined had the normal chromosome number of cabbage, suggesting that no spontaneous fusion between the two species had occurred during protoplast culture, and they showed no noticeable abnormalities in morphological features.
Abstract: A protocol for rapid and efficient plant regeneration from protoplasts of red cabbage was developed by a novel nurse culture method. When the protoplasts of red cabbage were cultured in modified MS medium containing various combinations of BA, NAA and 2,4-D, they did not continue dividing due to browning. However, they successfully divided and formed micro-calli at a high efficiency when they were mixed and co-cultured with those of tuber mustard at a 1:1 ratio. The presence of tuber mustard protoplasts used as nurse cells was essential for sustainable divisions and colony formation of red cabbage protoplasts. Red cabbage-like plantlets were regenerated from these protoplast-derived calli at a frequency ranging from 33 to 56% in all the experiments where three cultivars of red cabbage were tested. Over 120 protoplast-derived cabbage plants were transferred to the greenhouse, and they showed no noticeable abnormalities in morphological features. Chromosome observation revealed that all of the plants examined had the normal chromosome number of cabbage (2n = 18), suggesting that no spontaneous fusion between the two species had occurred during protoplast culture.
37 citations
TL;DR: This is the first report of enhanced protoplast division following an elevated temperature pretreatment, and some possible explanations for the observations are discussed.
37 citations
TL;DR: An efficient protoplast isolation and transient expression system for Magnolia denudata × Magnolia acuminata ‘Yellow River’ is developed and qPCR analysis of the MdeHSF1 transcript level in response to high temperature stress suggested that Mde HSF1 might be involved in regulating heat stress tolerance in ‘ Yellow River”.
Abstract: Magnolia is a woody ornamental plant, which is widely used in urban landscaping However, its lengthy juvenile period and recalcitrance to regeneration impedes functional characterization of its genes We developed an efficient protoplast isolation and transient expression system for Magnolia denudata × Magnolia acuminata ‘Yellow River’ The highest yield of protoplasts was obtained from young leaves digested in 3% Cellulase R10, 08% Macerozyme R10, 004% pectinase and 04 M mannitol enzymolysis solution for 6 h For transfection of protoplasts, 20% PEG4000 for 5 min was optimal To verify the protoplast system and begin to understand heat tolerance in Magnolia, a heat shock transcription factor MdeHSF1 was cloned from ‘Yellow River’, which belongs to the HSF subfamily A and has significant homology with AtHSFA1A Subcellular localization analysis indicated that MdeHSF1 was expressed in the cell nucleus Furthermore, qPCR analysis of the MdeHSF1 transcript level in response to high temperature stress suggested that MdeHSF1 might be involved in regulating heat stress tolerance in ‘Yellow River’ The described protocol provides a simple and straightforward method for isolating protoplast and exploring gene subcellular localization of MdeHSF1 in Magnolia This expands the new research of protoplast isolation and transfection in Magnolia
37 citations
TL;DR: Methods were developed for the reproducible production of high yields of stable protoplasts from monokaryotic and dikaryotic mycelia of Hebeloma cylindrosporum, indicating that protoplast will be useful to select physiological mutants from nonsporulating mycelian mutants and to create new strains by mating complementaryMycelia or by interspecific cell fusion.
Abstract: Methods were developed for the reproducible production of high yields of stable protoplasts from monokaryotic and dikaryotic mycelia of Hebeloma cylindrosporum. This is a prerequisite for mutation,...
37 citations