Topic
Protoplast
About: Protoplast is a research topic. Over the lifetime, 5474 publications have been published within this topic receiving 122468 citations.
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TL;DR: Enzyme localization studies indicate that a substantial amount of NADP-malate dehydrogenase (DH) in F. trinervia occurs not only in the mesophyll chloroplast, but also in the bundle sheath chloroplasts.
35 citations
TL;DR: Mesophyll protoplasts of Brassica oleracea var.
Abstract: Mesophyll protoplasts of Brassica oleracea var. botrytis were successfully transformed using polyethylene glycol (PEG). The success of plant transformation depended on both gene transfer and plant regeneration. Parameters, such as PEG and vector concentrations and heat shock conditions were tested in experiments on transient expression of the beta-glucuronidase (EC 3.2.1.31) gene and the most suitable conditions for DNA uptake were determined. Two antibiotic resistance marker genes for neomycin phosphotransferase (EC 2.7.1.95) and hygromycin phosphotransferase (EC 2.7.1.104), and three vector plasmids with different lengths were used to obtain stable transformants.
35 citations
TL;DR: The expression of antibiotic resistance and regeneration ability in the hybrids indicate that these are dominant or codominant traits in Lycopersicon and the sterility and subvitality of the resulting hybrids questions the value of somatic hybridization as a useful breeding approach.
35 citations
TL;DR: Protoplast of mutants agglutinated by polyethylene glycol are fused in macroscale by electrostimulation and the resulting stable recombinants show kanamycine resistance as well as formation of the typical brown pigment in a reasonable yield.
35 citations
TL;DR: The protoplast fusion of Aspergillus sojae was studied to develop a breeding system for the more desirable koji- molds in the production of shoyu (Japanese fermented soy-sauce) with special attention as to their enzyme productivities.
Abstract: The protoplast fusion of Aspergillus sojae was studied to develop a breeding system for the more desirable koji- molds in the production of shoyu (Japanese fermented soy-sauce) with special attention as to their enzyme productivities. Double-marker mutants, as to conidial color and nutritional requirement, were first derived from the parental strains selected from genealogically unrelated A. sojae cultures showing characteristic enzyme-productivities. Protoplasts of the mutants were obtained in high yields by using a combined enzyme-system composed of the enzyme preparation obtained from a culture filtrate of Bacillus circulans I AM 1165 and commercial chitinase (ICN). The protoplasts were fused in PEG 6000. Many of the fused products that grew on minimal regeneration plates were found to be heterokaryons. Stable heterozygous diploids with green conidia were induced from heterokaryons on UV treatment. One hundred and thirty diploid strains were obtained and their enzyme-productivities were assayed. The ac...
35 citations