Topic
Protoplast
About: Protoplast is a research topic. Over the lifetime, 5474 publications have been published within this topic receiving 122468 citations.
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TL;DR: Non-fixed protoplasts, having fluorescent nuclei, may be used in further protoplast studies, and the inhibition of DNA synthesis by hydroxyurea and duplication of DNA content per cell by colchicine could be demonstrated.
34 citations
TL;DR: Results indicate that the restriction-modification systems found in B. larvae NRRL B-3555 and B. subtilis 168 may be different.
Abstract: Protoplasts of Bacillus larvae NRRL b-3555 and Bacillus subtilis RM125 (restrictionless, modificationless mutant) were transfected with DNA from the B. larvae bacteriophage PBL1c in the presence of polyethylene glycol. B. subtilis 168 and Bacillus popilliae NRRL B-2309M protoplasts could not be transfected with PBL1c DNA. Protoplasts of B larvae NRRL B-3555 were transformed with plasmids pC194 and pHV33 in the presence of polyethylene glycol. The frequency of transformation was much higher when the plasmids were isolated from B. larvae NRRL B-3555 transformants than when they were isolated from B. subtilis 168. These results indicate that the restriction-modification systems found in B. larvae NRRL B-3555 and B. subtilis 168 may be different. Conditions for protoplast formation and cell wall regeneration were developed for B. popilliae NRRL B-2309S. However, no transformation occurred with plasmids pC194 and pHV33 (isolated from B. subtilis 168).
34 citations
TL;DR: Electrically induced protoplast fusion was used to produce somatic hybrids between Brassica napus L. and Sinapis alba L, indicating that the characteristic bands from S. alba were present in the hybrids.
Abstract: Electrically induced protoplast fusion was used to produce somatic hybrids between Brassica napus L. and Sinapis alba L. Seven hybrids were obtained and verified by the simple sequence repeat and cleaved amplified polymorphic sequence analysis of the gene fael, indicating that the characteristic bands from S. alba were present in the hybrids. The hybridity was also confirmed by chromosome number counting because the hybrids possessed 62 chromosomes, corresponding to the sum of fusion-parent chromosomes. Chromosome pairing at meiosis was predominantly normal, which led to high pollen fertility, ranging from 66% to 77%. All hybrids were grown to full maturity and could be fertilized and set seed after self-pollination or back-crosses with B. napus. The morphology of the hybrids resembled characteristics from both parental species. An analysis of the fatty acid composition in the seeds of F1 plants was conducted and the seeds were found to contain different amounts of erucic acid, ranging from 11.0% to 52.1%.
( Managing editor: Wei WANG)
34 citations
TL;DR: Slow-growing interspecific heterokaryons were isolated on minimal medium following the induced fusion of protoplasts from auxotrophic mutants of Penicillium chrysogenum and Penicilium cyaneo-fulvum and on transfer gave genetically stable colonies.
Abstract: Slow-growing interspecific heterokaryons were isolated on minimal medium following the induced fusion of protoplasts from auxotrophic mutants of Penicillium chrysogenum and Penicillium cyaneo-fulvum. After 5–7 days cultivation the heterokaryons produced vigorously growing sectors which on transfer gave genetically stable colonies. Cultivation of these colonies on a complete medium supplemented with p-fluorophenylalanine or benomyl broke down this stability and several different prototrophic and auxotrophic colony types were isolated. Many of these behaved as diploids or aneuploids showing sectoring either spontaneously, or in the presence of an haploidizing agent. Some of the latter isolates were recombinants for parental spore colour and auxotrophic markers.
34 citations
TL;DR: The results show that contrary to popular belief, mesophyll protoplasts of rice and perhaps other gramineous plants are capable of dedifferentiation and re-entry into the cell cycle, and are totipotent.
Abstract: We report induction of sustained divisions, colony formation and plant regeneration from mesophyll protoplasts isolated from the leaf base and sheath of rice seedlings. Protoplasts were purified on sucrose density gradients and cultured in modified liquid N6 medium in the presence of a feeder layer prepared from young rice-cell suspension cultures. Protoplasts formed cell walls and divided after 6 to 7 days giving rise to microcolonies which became macroscopic after 25 to 30 days. Nine to ten week old protocalli, on transfer to regeneration medium, formed somatic embryos that developed into shoots which were rooted on MS medium. Plantlets were recovered from four varieties, which on subsequent transfer to Yoshida's culture solution developed into healthy plants that were transferred to pots for further growth. Our results show that contrary to popular belief, mesophyll protoplasts of rice and perhaps other gramineous plants are capable of dedifferentiation and re-entry into the cell cycle, and are totipotent.
34 citations