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Protoplast

About: Protoplast is a research topic. Over the lifetime, 5474 publications have been published within this topic receiving 122468 citations.


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Journal ArticleDOI
TL;DR: Nitrate reductase deficient (NR-) cell lines were selected indirectly by their resistance to 40 mM chlorate in protoplast cultures of haploidNicotiana plumbaginifolia by tentatively classified as defective in the apoenzyme or the cofactor by the xanthine dehydrogenase activity and in vitro enzyme complementation.
Abstract: Nitrate reductase deficient (NR-) cell lines were selected indirectly by their resistance to 40 mM chlorate in protoplast cultures of haploidNicotiana plumbaginifolia. Frequency of the chlorate resistant clones was 5.8×10-5 in non-mutagenized cultures, which could be increased up to 25 times by treatment with N-ethyl-N-nitrosourea (NEU) or gamma irradiation.

97 citations

Journal ArticleDOI
TL;DR: A protocol for the efficient regeneration of plants from Arabidopsis thaliana protoplasts is presented and the differentially expressed genes identified are interesting candidates for investigating the molecular mechanisms underlying plant cell plasticity and totipotency.
Abstract: The molecular mechanisms underlying plant cell totipotency are largely unknown. Here, we present a protocol for the efficient regeneration of plants from Arabidopsis thaliana protoplasts. The specific liquid medium used in our study leads to a high rate of reentry into the cell cycle of most cell types, providing a powerful system to study dedifferentiation/regeneration processes in independent somatic cells. To identify the early events in the establishment of totipotency, we monitored the genome-wide transcript profiles of plantlets and protoplast-derived cells (PdCs) during the first week of culture. Plant cells rapidly dedifferentiated. Then, we observed the reinitiation and reorientation of protein synthesis, accompanied by the reinitiation of cell division and de novo cell wall synthesis. Marked changes in the expression of chromatin-associated genes, especially of those in the histone variant family, were observed during protoplast culture. Surprisingly, the epigenetic status of PdCs and well-established cell cultures differed, with PdCs exhibiting rare reactivated transposons and epigenetic changes. The differentially expressed genes identified in this study are interesting candidates for investigating the molecular mechanisms underlying plant cell plasticity and totipotency. One of these genes, the plant-specific transcription factor ABERRANT LATERAL ROOT FORMATION4, is required for the initiation of protoplast division.

96 citations

Journal ArticleDOI
TL;DR: Protoplasts were isolated from one Lolium perenne L. and three Festuca arundinacea Schreb and almost 300 Lolium protoplast derived cell colonies formed albino shoots and two colonies formed green shoots.

96 citations

Journal ArticleDOI
TL;DR: Examination of hyphal wall components of various races of Phytophthora infestans on protoplasts prepared from tuber tissues of potato cultivars having various resistance genes to the pathogen suggested a possibility that the recognition sites of theprotoplasts for the fungal components may reside in the protoplasmic membrane, resulting in occurrence of a hypersensitive reaction in potato cells.
Abstract: Effect of hyphal wall components of various races of Phytophthora infestans on protoplasts prepared from tuber tissues of potato cultivars having various resistance genes to the pathogen was investigated to know the interactions with surfaces of host cell membrane Reaction of protoplasts to the components was characterized by rapid protoplasmic aggregation followed by exolysis of the aggregate leaving a ghost of spherical membrane behind There was no significant difference in the physiological activities of the components from 7 races of the fungus Significant differences in the reactivity of protoplasts from different cultivars used was found regardless of the resistance genes of the cultivars There was a linear correlation between % of reacted protoplasts and concentration of fungal components (expressed as log) used for the treatments in each reaction system The higher the field resistance of the cultivars used, the more reactive to components were the protoplasts prepared from them Nearly 1 μg ml−1 of components was enough to initiate the reaction of protoplasts and more than 125 μg ml−1 elicited the reaction at maximum rate within 5 to 10 min after application These phenomena suggested a possibility that the recognition sites of the protoplasts for the fungal components may reside in the protoplasmic membrane, resulting in occurrence of a hypersensitive reaction in potato cells These results are discussed in connection with the host-parasite interaction in potato late blight

96 citations

Journal ArticleDOI
TL;DR: The experimental conditions for an efficient and reproducible enrichment of fusion products by flow cytometry, using protoplasts of different Brassica species as hybridization material, have been investigated and differentiated into shoots have been obtained from some of the hybrid calli.

95 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202358
2022153
202160
202060
201978
201855