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Protoplast

About: Protoplast is a research topic. Over the lifetime, 5474 publications have been published within this topic receiving 122468 citations.


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Journal ArticleDOI
TL;DR: A simplified method for the isolation of mitochondrial DNA of several plant species from either coleoptile or tissue cultured cells is described and employs differential low speed centrifugations for isolation of mitochondria and differential precipitations for the recovery of restrictable DNA.
Abstract: A simplified method for the isolation of mitochondrial DNA (mtDNA) of several plant species from either coleoptile or tissue cultured cells is described. The procedure does not require gradient ultracentrifugation or organic solvent extractions (such as phenol, chloroform, ether, etc.). Protoplast isolation is not required for the release of organelles from cell suspension cultured cells. The entire procedure can be performed in a single day and employs differential low speed centrifugations for isolation of mitochondria and differential precipitations for the recovery of restrictable DNA.

54 citations

Journal ArticleDOI
TL;DR: Not all hybrids regenerated from a common callus possessed identical mitochondrial genomes, suggesting that sorting out of mitochondrial populations in the callus may have been incomplete at the plant regeneration stage.
Abstract: Thirty somatic hybrids between Solanum tuberosum and Solanum brevidens were analysed for mitochondrial and chloroplast genome rearrangements. In all cases, the chloroplast genomes were inherited from one of the parental protoplast populations. No chloroplast DNA alterations were evident but a range of mitochondrial DNA alterations, from zero to extensive intra- and inter-molecular recombinations, were found. Such recombinations involved specific ‘recombination hot spots’ in the mitochondrial genome. Not all hybrids regenerated from a common callus possessed identical mitochondrial genomes, suggesting that sorting out of mitochondrial populations in the callus may have been incomplete at the plant regeneration stage. Sorting out of organelles in planta was not observed.

54 citations

Journal ArticleDOI
TL;DR: Since S. carnosus is apathogenic and an important organism in food technology, this organism could be a suitable gram-positive strain for molecular cloning.
Abstract: Several coagulase-negative staphylococci were investigated for their ability to undergo polyethylene glycolinduced protoplast-transformation with plasmid DNA. Among this group of bacteria it was found that S. carnosus TM300 was superior in this respect. Several plasmids isolated from different species were readily transformed with an efficiency ranging between 4×106 to 2×103 transformants per μg plasmid DNA. Since S. carnosus is apathogenic and an important organism in food technology, this organism could be a suitable gram-positive strain for molecular cloning.

54 citations

Journal ArticleDOI
TL;DR: The ontogeny of embryogenic callus in M. truncatula relates to explant orientation and is driven by the dynamics of pluripotent procambial cells, which proliferate and differentiate into vasculature, which contrasts with other species where totipotent embryo-forming initials mostly originate from procambials.

54 citations

Journal ArticleDOI
TL;DR: Preliminary experiments using electric-field mediated fusion suggest that electrofusion may offer significant advantages over the traditional chemical fusion in protoplasts of Nicotiana plumbaginifolia.
Abstract: Protoplast fusion studies between various auxotrophic mutants of Nicotiana plumbaginifolia were performed to optimize conditions for PEG-mediated fusion and to identify factors influencing the plant protoplast fusion process. Numerous parameters in the isolation, culture, and fusion of protoplasts were tested, and established fusion protocols were compared. Fusion rates, calculated on the basis of colony growth on selection medium (genetic complementation), ranged from 10−4 to 10−2. Conditions that allow rapid and reproducible fusions at the highest rates were established. Particular emphasis was given to fusion of mesophyll-derived protoplasts, for which the ability to regenerate fertile plants from fusion products was shown to be particularly high. Preliminary experiments using electric-field mediated fusion suggest that electrofusion may offer significant advantages over the traditional chemical fusion.

54 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202358
2022153
202160
202060
201978
201855