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Pseudomonas putida
About: Pseudomonas putida is a research topic. Over the lifetime, 6854 publications have been published within this topic receiving 230572 citations.
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TL;DR: Analysis of 13C-labelled phospholipid fatty acids (PLFAs) confirmed that heterotrophic bacteria may assimilate 13CO2 into cell macromolecules such as membrane lipids, indicating that assimilation of isotopically labelled CO2 can be used as a relatively simple measure of metabolic activity in heterotroph bacteria.
76 citations
TL;DR: Treatment with Pseudomonas putida WCS358r, a rifampicin‐resistant derivative of strain WCS358, significantly reduced fusarium wilt of carnation grown in rockwool if disease incidence was moderate, but not if Disease incidence was high.
Abstract: Treatment with Pseudomonas putida WCS358r, a rifampicin‐resistant derivative of strain WCS358, significantly reduced fusarium wilt of carnation grown in rockwool if disease incidence was moderate, but not if disease incidence was high. Differences in disease incidence could intentionally be established by varying the inoculum density of the pathogen Fusarium oxysporum f. sp. dianthi (Fod). The effectiveness of disease suppression by WCS358r increased with decrease of inoculum density and consequently decrease of disease incidence. WCS358r and a Tn5 marked derivative of WCS358 (B243) reduced fusarium wilt of carnation most effectively if a low iron availability for the pathogen was established by adding unferrated or only partially ferrated ethylenediamine [di(o‐hydroxyphenylacetic) acid]. A Tn5 mutant of WCS358 defective in siderophore biosynthesis (JM218) did not reduce disease incidence. Siderophore production and inhibition of Fod by WCS358r in vitro decreased with increasing iron availability, support...
76 citations
TL;DR: The anthranilate and benzoate promoters are found to be useful for tightly controlling recombinant gene expression at both small (< 1 L) and large (20 L) fermentation scales.
Abstract: In an effort to identify alternate recombinant gene expression systems in Pseudomonas fluorescens, we identified genes encoding two native metabolic pathways that were inducible with inexpensive compounds: the anthranilate operon (antABC) and the benzoate operon (benABCD). The antABC and benABCD operons were identified by homology to the Acinetobacter sp. anthranilate operon and Pseudomonas putida benzoate operon, and were confirmed to be regulated by anthranilate or benzoate, respectively. Fusions of the putative promoter regions to the E. coli lacZ gene were constructed to confirm inducible gene expression. Each operon was found to be controlled by an AraC family transcriptional activator, located immediately upstream of the first structural gene in each respective operon (antR or benR). We have found the anthranilate and benzoate promoters to be useful for tightly controlling recombinant gene expression at both small (< 1 L) and large (20 L) fermentation scales.
76 citations
76 citations
TL;DR: Fibrillar structures originating from the plant cell wall in the intercellular spaces of leaves of Leaves of `Red Kidney' bean engulfed a saprophytic bacterium, Pseudomonas putida, after its initial attachment to the host walls.
Abstract: Fibrillar structures, originating from the plant cell wall in the intercellular spaces of leaves of ;Red Kidney' bean, Phaseolus vulgaris L., engulfed a saprophytic bacterium, Pseudomonas putida, after its initial attachment to the host walls. Phytopathogenic bacteria, Pseudomonas phaseolicola and Pseudomonas tomato, did not adhere to the plant cell wall nor were they encapsulated. Bean lectins may be involved in the attachment and encapsulation processes.
76 citations