Topic
Pseudomonas putida
About: Pseudomonas putida is a research topic. Over the lifetime, 6854 publications have been published within this topic receiving 230572 citations.
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TL;DR: A bacterium, Pimelobacter sp.
Abstract: A bacterium, Pimelobacter sp. R48, isolated from soil, showed the ability to produce trehalose from maltose. The partially purified enzyme from a cell-free extract catalyzed the conversion of maltose into trehalose without requiring phosphate. The enzyme was considered to be a new intramolecular glucosyltransferase. The enzyme was also tentatively found to exist in Pseudomonas putida H262 isolated from soil and in some Thermus strains.
72 citations
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TL;DR: Cloned the l-methioninase gene from Pseudomonas putida and isolated pure and abundant recombinant enzyme will allow in vivo studies on the antitumor activity and the potential toxicity of enzymatic methionine depletion.
Abstract: Methionine dependency has been reported in cancer cell lines and primary tumors. Thus, L-methionine deprivation might have potential value for the treatment of human cancers with a methionine requirement. L-Methionine-alpha-deamino-gamma-mercaptomethane-lyase has been reported to decrease plasma methionine levels and to inhibit tumor growth in experimental animals but has not been studied extensively because sufficient homogeneous enzyme was not available. In this study, we cloned the L-methioninase gene from Pseudomonas putida and isolated pure and abundant recombinant enzyme. Both L-methionine and L-cysteine in culture medium were completely degraded by 1 unit/ml purified enzyme. Two hundred and fifty units/kg L-methioninase administered i.v. to mice yielded 0.7 unit/ml of plasma concentration and lowered total plasma sulfur-containing amino acids by more than 75%. Although sensitivity to enzymatic methionine depletion differed among cell lines, leukemia cell lines were generally more sensitive than solid tumor cell lines. The availability of pure recombinant L-methioninase will allow in vivo studies on the antitumor activity and the potential toxicity of enzymatic methionine depletion.
72 citations
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TL;DR: In this article, an enzyme-catalysed kinetic resolution and asymmetric dihydroxylation routes to enantiopure cis-diol metabolites of arenes and benzocycloalkenes of either absolute configuration have been developed using appropriate strains of the bacterium Pseudomonas putida.
Abstract: Enzyme-catalysed kinetic resolution and asymmetric dihydroxylation routes to enantiopure cis-diol metabolites of arenes and benzocycloalkenes of either absolute configuration have been developed using appropriate strains of the bacterium Pseudomonas putida.
72 citations
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TL;DR: The isolation of a new malathion-degrading bacteria from soils in Egypt that may be very well adapted to the climatic and environmental conditions of the country is reported, and a new carboxylesterase gene is reported.
Abstract: Five malathion-degrading bacterial strains were enriched and isolated from soil samples collected from different agricultural sites in Cairo, Egypt. Malathion was used as a sole source of carbon (50 mg/l) to enumerate malathion degraders, which were designated as IS1, IS2, IS3, IS4, and IS5. They were identified, based on their morphological and biochemical characteristics, as Pseudomonas sp., Pseudomonas putida, Micrococcus lylae, Pseudomonas aureofaciens, and Acetobacter liquefaciens, respectively. IS1 and IS2, which showed the highest degrading activity, were selected for further identification by partial sequence analysis of their 16S rRNA genes. The 16S rRNA gene of IS1 shared 99% similarity with that of Alphaprotoebacterium BAL284, while IS2 scored 100% similarity with that of Pseudomonas putida 32zhy. Malathion residues almost completely disappeared within 6 days of incubation in IS2 liquid cultures. LC/ESI-MS analysis confirmed the degradation of malathion to malathion monocarboxylic and dicarboxylic acids, which formed as a result of carboxylesterase activity. A carboxylesterase gene (CE) was amplified from the IS2 genome by using specifically designed PCR primers. The sequence analysis showed a significant similarity to a known CE gene in different Pseudomonas sp. We report here the isolation of a new malathion-degrading bacteria from soils in Egypt that may be very well adapted to the climatic and environmental conditions of the country. We also report the partial cloning of a new CE gene. Due to their high biodegradation activity, the bacteria isolated from this work merit further study as potential biological agents for the remediation of soil, water, or crops contaminated with the pesticide malathion.
72 citations
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TL;DR: In this paper, a chemostat culture of Pseudomonas putida GPo1 under well-defined dual-(C,N)-nutrient limited growth conditions was used to produce tailor-made copolymers.
Abstract: Mixtures of 5-phenylvalerate, octanoate, and 10-undecenoate were fed to a chemostat culture (dilution rate = 0.1 h-1) of Pseudomonas putida GPo1 under well-defined dual-(C,N)-nutrient limited growth conditions. Five new, tailor-made copolymers were produced and consisted of poly(3-hydroxy-5-phenylvalerate-co-3-hydroxyalkanoates-co-3-hydroxy-ω-alkenoates), poly(HP-co-HA-co-HE), with increasing amounts of aromatic side chains (A, 0%; B, 3%; C, 19%; D, 42%; and E, 59%), approximately 10 mol % unsaturated side chains, and decreasing amounts of saturated side chains. On the basis of NMR analysis of polymer E, it was concluded that the incorporation of the substrates occurred randomly. The HP-content determined the glass transition temperature, which increased linearly from −38.7 °C for poly(0%HP-co-90%HA-co-10%HE) to −6.0 °C for poly(59%HP-co-31%HA-co-10%HE).
72 citations