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Pseudomonas putida
About: Pseudomonas putida is a research topic. Over the lifetime, 6854 publications have been published within this topic receiving 230572 citations.
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TL;DR: The value of P. putida as a bacterial chassis of choice for contemporary, industrially-oriented metabolic engineering is addressed and the biochemical properties that make this bacterium adequate for hosting biotransformations involving redox reactions as well as toxic compounds and intermediates are discussed.
187 citations
TL;DR: The potential of different Pseudomonas strains to utilize heterologous siderophores was compared with their competitiveness in the rhizosphere of radish to find out the winners and losers.
Abstract: In this study, the potential of different Pseudomonas strains to utilize heterologous siderophores was compared with their competitiveness in the rhizosphere of radish. This issue was investigated ...
187 citations
Journal Article•
TL;DR: The data suggest that seed inoculation of barley with plant PGPR increased root weight by 17.9%-32.1% as compared to the control, and increased shoot weight by 28.8%-54.2%, depending on the species.
Abstract: This study was conducted with barley in greenhouse conditions in order to investigate seed inoculation with 5 different N2-fixing (Bacillus licheniformis RC02, Rhodobacter capsulatus RC04, Paenibacillus polymyxa RC05, Pseudomonas putida RC06, and Bacillus OSU-142) and 2 different phosphate-solubilising (Bacillus megaterium RC01 and Bacillus M-13) bacteria in comparison to control and mineral fertiliser (N and P) application. Among the strains used in the present study, 6 plant growth promoting rhizobacteria (PGPR) stimulated indole acetic acid (IAA) production and 3 of them stimulated phosphate solubilisation; all bacterial strains fixed N2 and significantly increased the growth of barley. Available phosphate in soil was significantly increased by seed inoculation with Bacillus M-13 and B. megaterium RC01. Maximum NO3-N was found in soil after inoculation with N2-fixing Bacillus OSU-142, followed by P. polymyxa RC05 and R. capsulatus RC04. Total culturable bacteria count increased in all treatments with time, whereas N2-fixing bacteria decreased with time, except with B. megaterium RC01 inoculation. The data suggest that seed inoculation of barley with plant PGPR increased root weight by 17.9%-32.1% as compared to the control, and increased shoot weight by 28.8%-54.2%, depending on the species. N2-fixing bacterial inoculation significantly increased uptake of N, Fe, Mn, and Zn by barley. The production of hormones is suggested to be one of the mechanisms by which PGPR stimulate barley growth. Effective Bacillus species, such as OSU-142, RC07, M-13, P. polymyxa RC05, P. putida RC06, and R. capsulatus RC04, may be used in agriculture.
187 citations
TL;DR: A new Pseudomonas species was isolated from cultured ayu (Plecoglossus altivelis) with bacterial haemorrhagic ascites that reduced nitrate to nitrite and was distinguished phenotypically from PseUDomonas putida biovar A by utilization of D-malate, L-(+)-tartrate, m-Tartrate and nicotinate.
Abstract: A new Pseudomonas species, for which the name Pseudomonas plecoglossicida is proposed, was isolated from cultured ayu (Plecoglossus altivelis) with bacterial haemorrhagic ascites. The causative agent was similar to Pseudomonas putida biovar A in its phenotypic characteristics and on the basis of 16S rRNA gene sequence analysis, but it reduced nitrate to nitrite. Furthermore, it was distinguished phenotypically from Pseudomonas putida biovar A by utilization of D-malate, L-(+)-tartrate, m-tartrate and nicotinate. The levels of DNA-DNA hybridization between the isolate strain FPC 951T and other reference strains of Pseudomonas species, including Pseudomonas putida, were less than 50%. The G+C content of the DNA of FPC 951T was 62.8 mol%. Strain FPC 951T (= ATCC 700383T) is designated the type strain of the new species.
186 citations
TL;DR: The possibility of electric field-mediated transformation ("electroporation") of a gram-positive bacterium and two gram-negative bacteria with plasmid DNA was investigated and results are given.
186 citations