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Pseudomonas putida

About: Pseudomonas putida is a research topic. Over the lifetime, 6854 publications have been published within this topic receiving 230572 citations.


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TL;DR: In this paper, peak assignments in 31 P NMR spectra of alkaline soil extracts were analyzed and it was shown that the resonance at 0.5-1.9ppm was caused by DNA-P and the other diesters were represented by teichoic acids.
Abstract: This study involved a critical assessment of peak assignments in 31 P NMR spectra of alkaline soil extracts and included 31 P NMR spectroscopy of (1) 500 mM NaOH solutions of RNA, DNA, and lecithin; (2) 50 mM H 2 SO 4 , 500 mM NaHCO 3 (pH 8.5), and 100 mM NaOH extracts from Pseudomonas putida (Gram-negative bacterium), Bacillus subtilis (Gram-positive bacterium), Penicillium citrinum (a fungus), Aspergillus niger (a fungus), and leaves of Betula pubescens , Picea abies , and Pinus cembra ; (3) 100 mM NaOH solutions of evaporated methanol–chloroform extracts from B. subtilis , P. putida , B. pubescens leaves, and from organic and mineral soil horizons; (4) 100 mM NaOH and 50 mM H 2 SO 4 extracts from delipidized bacterial cells, and 100 mM NaOH extracts from delipidized birch leaves and soil samples. Results showed that the resonance at 0 ppm, previously assigned to phospholipids and nucleic acids, was caused by DNA-P. Resonances of phospholipids of plant and microbial origin were observed at about 1.5–1.7 and 0.6–0.7 ppm, in regions previously assigned to teichoic acid-P by various authors. Non-lipidic compounds extracted from B. subtilis and resonating at 1.9 ppm probably were represented by teichoic acids. 31 P NMR spectroscopy cannot differentiate signals derived from P of phospholipids and non-lipidic compounds in the low field of the diester region of spectra of alkaline extracts from soils, while quantitative differentiation of DNA-P and the other diesters seems quite a simple task. An unknown resonance at −1.5 ppm remained unidentified. The real concentrations of diester-P in soils can be considerably underestimated and those of monoester-P overestimated, when analyzed in alkaline extracts, because of almost complete hydrolysis of RNA and partial hydrolysis of phospholipids to monoesters.

155 citations

Journal ArticleDOI
TL;DR: Pseudomonas sp. strain JS150 was isolated as a nonencapsulated variant of Pseudomanas putida strain JS1 that contains the genes for the degradative pathways of a wide range of substituted aromatic compounds as mentioned in this paper.
Abstract: Pseudomonas sp. strain JS150 was isolated as a nonencapsulated variant of Pseudomonas sp. strain JS1 that contains the genes for the degradative pathways of a wide range of substituted aromatic compounds. Pseudomonas sp. strain JS150 grew on phenol, ethylbenzene, toluene, benzene, naphthalene, benzoate, p-hydroxybenzoate, salicylate, chlorobenzene, and several 1,4-dihalogenated benzenes. We designed experiments to determine the conditions required for induction of the individual pathways and to determine whether multiple substrates could be biodegraded simultaneously. Oxygen consumption studies with whole cells and enzyme assays with cell extracts showed that the enzymes of the meta, ortho, and modified ortho cleavage pathways can be induced in strain JS150. Strain JS150 contains a nonspecific toluene dioxygenase with a substrate range similar to that found in strains of Pseudomonas putida. The presence of the dioxygenase along with multiple pathways for metabolism of substituted catechols allows facile extension of the growth range by spontaneous mutation and degradation of mixtures of substituted benzenes and phenols. Chlorobenzene-grown cells of strain JS150 degraded mixtures of chlorobenzene, benzene, toluene, naphthalene, trichloroethylene, and 1,2- and 1,4-dichlorobenzenes in continuous culture. Under similar conditions, phenol-grown cells degraded a mixture of phenol, 2-chloro-, 3-chloro, and 2,5-dichlorophenol and 2-methyl- and 3-methylphenol. These results indicate that induction of appropriate biodegradative pathways in strain JS150 permits the biodegradation of complex mixtures of aromatic compounds.

155 citations

Journal ArticleDOI
TL;DR: Findings indicate that the strain mt-2 possesses the genetic capacity for enzymes of both the meta- and ortho-cleavage pathways of benzoate degradation, but its phenotypic expression is the meta pathway.
Abstract: Benzoate-grown cells of Pseudomonas putida(arvilla) mt-2 contain both metapyrocatechase and pyrocatechase activities, although the former activity is much higher than that of the latter. A spontaneous mutant deficient in metapyrocatechase and 2-hydroxymuconic semialdehyde hydrolyase, the first two enzymes in the meta-cleavage pathway of the ring of catechol, has been isolated from this strain. This mutant grows well on a minimal medium containing benzoate as a sole carbon source and has the high activity of pyrocatechase. These findings indicate that the strain mt-2 possesses the genetic capacity for enzymes of both the meta- and ortho-cleavage pathways of benzoate degradation, but its phenotypic expression is the meta pathway.

154 citations

Journal ArticleDOI
TL;DR: Bioaugmentation can constitute an effective strategy for cleanup of soils which are poor in nutrients and microbial activity, such as those of the B horizon, and indicates that the observed change in the community was due to proliferation of transconjugants formed in soil.
Abstract: Transfer of the 2,4-dichlorophenoxyacetic acid (2,4-D) degradation plasmids pEMT1 and pJP4 from an introduced donor strain, Pseudomonas putida UWC3, to the indigenous bacteria of two different horizons (A horizon, depth of 0 to 30 cm; B horizon, depth of 30 to 60 cm) of a 2,4-D-contaminated soil was investigated as a means of bioaugmentation. When the soil was amended with nutrients, plasmid transfer and enhanced degradation of 2,4-D were observed. These findings were most striking in the B horizon, where the indigenous bacteria were unable to degrade any of the 2,4-D (100 mg/kg of soil) during at least 22 days but where inoculation with either of the two plasmid donors resulted in complete 2,4-D degradation within 14 days. In contrast, in soils not amended with nutrients, inoculation of donors in the A horizon and subsequent formation of transconjugants (105 CFU/g of soil) could not increase the 2,4-D degradation rate compared to that of the noninoculated soil. However, donor inoculation in the nonamended B-horizon soil resulted in complete degradation of 2,4-D within 19 days, while no degradation at all was observed in noninoculated soil during 89 days. With plasmid pEMT1, this enhanced degradation seemed to be due only to transconjugants (105 CFU/g of soil), since the donor was already undetectable when degradation started. Denaturing gradient gel electrophoresis (DGGE) of 16S rRNA genes showed that inoculation of the donors was followed by a shift in the microbial community structure of the nonamended B-horizon soils. The new 16S rRNA gene fragments in the DGGE profile corresponded with the 16S rRNA genes of 2,4-D-degrading transconjugant colonies isolated on agar plates. This result indicates that the observed change in the community was due to proliferation of transconjugants formed in soil. Overall, this work clearly demonstrates that bioaugmentation can constitute an effective strategy for cleanup of soils which are poor in nutrients and microbial activity, such as those of the B horizon.

153 citations

Journal ArticleDOI
13 Mar 2013-PLOS ONE
TL;DR: Genes potentially involved in plant growth promotion such as indole-3-acetic acid (IAA) biosynthesis, trehalose production, siderophore production, acetoin synthesis, and phosphate solubilization were determined and genes that contribute to the environmental fitness of UW4 were observed.
Abstract: The plant growth-promoting bacterium (PGPB) Pseudomonas sp. UW4, previously isolated from the rhizosphere of common reeds growing on the campus of the University of Waterloo, promotes plant growth in the presence of different environmental stresses, such as flooding, high concentrations of salt, cold, heavy metals, drought and phytopathogens. In this work, the genome sequence of UW4 was obtained by pyrosequencing and the gaps between the contigs were closed by directed PCR. The P. sp. UW4 genome contains a single circular chromosome that is 6,183,388 bp with a 60.05% G+C content. The bacterial genome contains 5,423 predicted protein-coding sequences that occupy 87.2% of the genome. Nineteen genomic islands (GIs) were predicted and thirty one complete putative insertion sequences were identified. Genes potentially involved in plant growth promotion such as indole-3-acetic acid (IAA) biosynthesis, trehalose production, siderophore production, acetoin synthesis, and phosphate solubilization were determined. Moreover, genes that contribute to the environmental fitness of UW4 were also observed including genes responsible for heavy metal resistance such as nickel, copper, cadmium, zinc, molybdate, cobalt, arsenate, and chromate. Whole-genome comparison with other completely sequenced Pseudomonas strains and phylogeny of four concatenated “housekeeping” genes (16S rRNA, gyrB, rpoB and rpoD) of 128 Pseudomonas strains revealed that UW4 belongs to the fluorescens group, jessenii subgroup.

153 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023184
2022345
2021182
2020246
2019226
2018206