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Pseudomonas putida
About: Pseudomonas putida is a research topic. Over the lifetime, 6854 publications have been published within this topic receiving 230572 citations.
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TL;DR: None of the strains suppressed disease when mixed together with the pathogen through the soil or when seeds or seedlings were treated with the strains one to four weeks before transfer into soil infested with R. solanacearum, indicating that siderophore-mediated competition for iron can contribute but is not effective enough to suppress bacterial wilt in Eucalyptus.
111 citations
TL;DR: Here, inoculation with P. putida W619-TCE had a less pronounced positive effect on plant growth and TCE phytotoxicity, but resulted in strongly reduced amounts of TCE in leaves and roots of plants exposed to 400 mg l(-1) TCE, accompanied by a lowered evapotranspiration of T CE.
111 citations
TL;DR: The nucleotide sequence of the genes from Pseudomonas putida encoding oxidation of benzene to catechol was determined and the relation between the product of each cistron and the components of the benzene oxidation enzyme system is discussed.
Abstract: The nucleotide sequence of the genes from Pseudomonas putida encoding oxidation of benzene to catechol was determined. Five open reading frames were found in the sequence. Four corresponding protein molecules were detected by a DNA-directed in vitro translation system. Escherichia coli cells containing the fragment with the four open reading frames transformed benzene to cis-benzene glycol, which is an intermediate of the oxidation of benzene to catechol. The relation between the product of each cistron and the components of the benzene oxidation enzyme system is discussed.
111 citations
TL;DR: Talc-based formulations of the two strains of Pseudomonas putida were tested separately and together for their ability to suppress the development of Fusarial wilt of muskmelon, caused by Fusarium oxysporum f.
Abstract: Talc-based formulations of the two strains of Pseudomonas putida were tested separately and together for their ability to suppress the development of Fusarial wilt of muskmelon, caused by Fusarium oxysporum f. sp. melonis, under in vivo conditions in 2 consecutive years. In field trials conducted in 2001 and 2002, control efficacy achieved by seed treatment by the formulations of P. putida strain 30 was 63% and 46-50% for strain 180 at 90 days after transplanting. Seed treatment by the mixture of P. putida strains did not achieve as much effectiveness as biocontrol agents applied separately. Benomyl, used as reference chemical in both years, was less effective in reduction of the wilt severity of F. oxysporum f. sp. melonis than the bioformulation treatments.
111 citations
TL;DR: A novel membrane-localized regulator, MorA, is described that controls the timing of flagellar development and affects motility, chemotaxis, and biofilm formation in Pseudomonas putida and pseudomonas aeruginosa.
Abstract: Assembly of bacterial flagella is developmentally important during both planktonic cell growth and biofilm formation. Flagellar biogenesis is complex, requiring coordinated expression of over 40 genes, and normally commences during the log-to-stationary transition phase. We describe here a novel membrane-localized regulator, MorA, that controls the timing of flagellar development and affects motility, chemotaxis, and biofilm formation in Pseudomonas putida. MorA is conserved among diverse Pseudomonas species, and homologues are present in all Pseudomonas genomes sequenced thus far. In P. putida, the absence of MorA derepresses flagellar development, which leads to constitutive formation of flagella in the mutant cells in all growth phases. In Pseudomonas aeruginosa, the absence of MorA led to a reduction in biofilm formation. However, unlike the motility of P. putida, the motility of the P. aeruginosa mutants was unaffected. Our data illustrate a novel developmentally regulated sensory and signaling pathway for several properties required for virulence and ecological fitness of Pseudomonas species.
111 citations