Topic
Pyruvate dehydrogenase kinase
About: Pyruvate dehydrogenase kinase is a research topic. Over the lifetime, 4224 publications have been published within this topic receiving 161052 citations. The topic is also known as: [pyruvate dehydrogenase (lipoamide)] kinase & pyruvate dehydrogenase (lipoamide) kinase.
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TL;DR: P-enolpyruvate carboxykinase mutants have established that the enzyme is needed for gluconeogenesis and, under normal growth conditions, for sporulation, and can be restored by the continuous feeding of gluconate.
84 citations
TL;DR: Tissue specific expression of these enzymes and/or control of their association with the complex will probably prove to be important for the long term regulation of the activity of the pyruvate dehydrogenase complex.
83 citations
TL;DR: In this paper, the presence of a pyruvate(-)-OH(-) exchange transporter which supplies the mitochondrion with oxidizable substrate was found in animal mitochondria, and malate and NADH were inhibited by alpha-cyano-4-hydroxy-cinnamic acid.
Abstract: Pyruvate oxidation and swelling in pyruvate solutions by corn (Zea mays) mitochondria were inhibited by alpha-cyano-4-hydroxy-cinnamic acid, an inhibitor of pyruvate transport in animal mitochondria; however, there was no inhibition of pyruvate dehydrogenase activity, and malate and NADH oxidation were not affected. These results suggest the presence of a pyruvate(-)-OH(-) exchange transporter which supplies the mitochondrion with oxidizable substrate. Lactate appears to be transported also, but not dicarboxylate anions or inorganic phosphate. The rate of pyruvate transport was much slower than that of malate, however, and valinomycin was required to elicit appreciable swelling in potassium pyruvate.Malate oxidation contributed significantly to respiration supported by pyruvate plus malate, and malate did not act solely as a "sparker" for pyruvate oxidation. NAD(+)-malic enzyme activity was found in sonicated preparations, and comparison of O(2) consumption with CO(2) released from 1-(14)C-pyruvate indicated that transported malate was being converted to pyruvate, particularly as the malate to pyruvate ratio increased. The results suggest that pyruvate transport becomes limiting under conditions of high energy demand, but that rapid malate transport makes up the difference, supplying pyruvate via malic enzyme and replenishing losses of tricarboxylic acid cycle intermediates.
83 citations
TL;DR: The rate of immediate myofibrillar MgADP rephosphorylation in the endogenous creatine-kinase reaction was observed to be governed by the concentration of phosphocreatine in accordance with the kinetics of this enzyme.
83 citations
TL;DR: Recombinant p45 protein, expressed in Escherichia coli and purified to homogeneity, catalyzed the phosphorylation and inactivation of kinase-depleted pyruvate dehydrogenase complex, indicating that p45 and p48 correspond to different isoforms of pyruVate dehydrogensase kinase.
83 citations