Topic
Pyruvate dehydrogenase kinase
About: Pyruvate dehydrogenase kinase is a research topic. Over the lifetime, 4224 publications have been published within this topic receiving 161052 citations. The topic is also known as: [pyruvate dehydrogenase (lipoamide)] kinase & pyruvate dehydrogenase (lipoamide) kinase.
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TL;DR: PGC‐1 alpha and ERR gamma bind by surface plasmon resonance to form H2O2 ’s ‘spatially reprograming signal’.
74 citations
01 Jan 1998
TL;DR: It is found that in respiring root tips, anaplerotic phosphoenolpyruvate carboxylase activity was high relative to ME, and therefore did not limit synthesis of pyruVate by ME, which is discussed with respect to malate and pyruvates utilization by isolated mitochondria and intracellular pH regulation under hypoxia.
Abstract: In vivo pyruvate synthesis by malic enzyme (ME) and pyruvate kinase and in vivo malate synthesis by phosphoenolpyruvate carboxylase and the Krebs cycle were measured by 13C incorporation from [1-'3C]glucose into glucose-6-phosphate, alanine, glutamate, aspartate, and malate. These metabolites were isolated from maize (Zea mays L.) root tips under aerobic and hypoxic conditions. '3CNuclear magnetic resonance spectroscopy and gas chromatographymass spectrometry were used to discern the positional isotopic distribution within each metabolite. This information was applied to a simple precursor-product model that enabled calculation of specific metabolic fluxes. In respiring root tips, ME was found to contribute only approximately 3% of the pyruvate synthesized, whereas pyruvate kinase contributed the balance. The activity of ME increased greater than 6-fold early in hypoxia, and then declined coincident with depletion of cytosolic malate and aspartate. We found that in respiring root tips, anaplerotic phosphoenolpyruvate carboxylase activity was high relative to ME, and therefore did not limit synthesis of pyruvate by ME. The significance of in vivo pyruvate synthesis by ME is discussed with respect to malate and pyruvate utilization by isolated mitochondria and intracellular pH regulation under hypoxia.
74 citations
TL;DR: The rate of pyruvate kinase flux in the intact cell is estimated by a new procedure, involving trapping of 14C from NaH14CO3 in a large pyruVate + lactate pool, and calculation of the specific activity of phosphoenol pyruviate.
74 citations
TL;DR: The data in this study suggest that the enzyme pyruvate kinase in the oyster occurs in tissue specific multimolecular forms and that the kinetic properties of each isozyme seem to gear in well with the over-all metabolism of the tissue.
74 citations
TL;DR: The ketone body formation from pyruvate is more easily suppressed by the citric acid cycle than is the formation of ketone bodies from the carnitine-bound fatty acids, which develops in conditions with increased fatty acid mobilization and utilization.
74 citations