Topic
Pyruvate dehydrogenase kinase
About: Pyruvate dehydrogenase kinase is a research topic. Over the lifetime, 4224 publications have been published within this topic receiving 161052 citations. The topic is also known as: [pyruvate dehydrogenase (lipoamide)] kinase & pyruvate dehydrogenase (lipoamide) kinase.
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TL;DR: Two additional methods for the detection of abnormal pyruvate kinase in hemolysates are described: heat stability test and activation studies with the allosteric effector fructose 1,6-diphosphate.
63 citations
TL;DR: Pyruvate carboxylase was partially purified from Aspergillus niger and the properties were studied, and the enzyme was shown to be a biotin-containing enzyme by its in activation by avidin and protection against such inactivation by biotin.
Abstract: Pyruvate carboxylase was partially purified from Aspergillus niger and the properties were studied. The enzyme was found to be cold-labile and protected by 25% glycerol. The pH optimum was determin...
63 citations
TL;DR: DCA treatment was able to re-sensitize gastric cancer cells with hypoxia-induced resistance to 5-FU through the alteration of glucose metabolism, as well as in PDK1 knockdown cell lines.
63 citations
TL;DR: The present review describes some of the original work of Eric Newsholme, its relevance to metabolic homoeostasis and disease and application to present state-of-the-art studies, which generate substantial amounts of data that are extremely difficult to interpret without a fundamental understanding of regulatory principles.
Abstract: The importance of metabolic pathways for life and the nature of participating reactions have challenged physiologists and biochemists for over a hundred years. Eric Arthur Newsholme contributed many original hypotheses and concepts to the field of metabolic regulation, demonstrating that metabolic pathways have a fundamental thermodynamic structure and that near identical regulatory mechanisms exist in multiple species across the animal kingdom. His work at Oxford University from the 1970s to 1990s was groundbreaking and led to better understanding of development and demise across the lifespan as well as the basis of metabolic disruption responsible for the development of obesity, diabetes and many other conditions. In the present review we describe some of the original work of Eric Newsholme, its relevance to metabolic homoeostasis and disease and application to present state-of-the-art studies, which generate substantial amounts of data that are extremely difficult to interpret without a fundamental understanding of regulatory principles. Eric's work is a classical example of how one can unravel very complex problems by considering regulation from a cell, tissue and whole body perspective, thus bringing together metabolic biochemistry, physiology and pathophysiology, opening new avenues that now drive discovery decades thereafter.
* ACC, : acetyl-CoA carboxylase; AMPK, : AMP-activated protein kinase; CaMKK, : calmodulin-dependent protein kinase kinase; GLUT, : glucose transporter; GDH, : glutamate dehydrogenase; GLS, : glutaminase; GS, : glutamine synthetase; HIF, : hypoxia-inducible factor; HK, : hexokinase; LKB1, : liver kinase B1; mTOR, : mammalian target of rapamycin; mTORC1, : mTOR complex 1; PDHK, : pyruvate dehydrogenase kinase; PFK, : phosphofructokinase; SIRT4, : sirtuin 4; SLC, : solute carrier; TAK, : transforming growth factor-beta-activating kinase; TCA, : tricarboxylic acid
63 citations
TL;DR: It is suggested that the chicken liver enzyme carries specific regulatory sites at which α-ketoglutarate interacts, while the inhibition patterns observed for l-glutamate appear generally consistent with the proposal that this metabolite interacts at the α- keto acid site when the enzyme is in the form E-biotin ∼ CO2.
63 citations