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Pyruvate kinase

About: Pyruvate kinase is a research topic. Over the lifetime, 5683 publications have been published within this topic receiving 180020 citations. The topic is also known as: ATP:pyruvate 2-O-phosphotransferase & phosphoenolpyruvate kinase.


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Journal ArticleDOI
TL;DR: The antagonistic action of zinc and cAMP on glycolysis together with the rapid and marked decrease in free zinc concentration induced by glucagon (cAMP) may indicate an as yet unknown role of zinc as an important mediator of regulation of carbohydrate metabolism.

84 citations

Journal ArticleDOI
TL;DR: In this article, electron microscopic immunogold labeling was carried out in isolated sarcoplasmic reticulum (SR) vesicles using specific primary antibodies against selected glycolytic enzymes and Ca 2 1 -ATPase, and appropriate secondary antibodies labeled with 6-nm or 12-nm gold particles.
Abstract: SUMMARY We have previously obtained indirect evidence that sarcoplasmic reticulum (SR) vesicles from cardiac and skeletal muscle contain the complete chain of glycolytic enzymes from aldolase to pyruvate kinase. To investigate directly whether pyruvate kinase and other glycolytic enzymes are anatomically associated with the SR, electron microscopic immunogold labeling studies were carried out in isolated SR vesicles using specific primary antibodies against selected glycolytic enzymes and Ca 2 1 -ATPase, and appropriate secondary antibodies labeled with 6-nm or 12-nm gold particles. Pyruvate kinase was broadly dispersed on the cytoplasmic side of the SR membrane of both cardiac and skeletal muscle vesicles. With 6-nm gold particles, the density of binding to pyruvate kinase was 2522 6 445 and 4171 6 1379 particles/ m m 2 for cardiac and skeletal muscle SR, respectively. Binding densities to Ca 2 1 -ATPase were similar (2550 6 639 particles/ m m 2 for cardiac SR, 3877 6 408 particles/ m m 2 for skeletal muscle SR). Immunogold labeling of ultrathin sections indicated that pyruvate kinase was attached to the SR membrane and located immediately adjacent to the Ca 2 1 -ATPase. Aldolase and glyceraldehyde phosphate dehydrogenase were also found to be attached to the cytoplasmic side of SR vesicles and located in close proximity to Ca 2 1 -ATPase. These results provide the first ultrastructural evidence that glycolytic enzymes are anatomically associated with SR membranes and located near the SR Ca 2 1 -ATPase. The results further support the hypothesis that ATP generated by SR-associated glycolytic enzymes is coupled to SR Ca 2 1 active transport. (J Histochem Cytochem 46:419‐427, 1998)

84 citations

Journal ArticleDOI
TL;DR: It appears that the properties of thallium-205 are uniquely suitable in this regard for probing macromolecular environment in the presence of paramagnetic ions.

84 citations

Journal ArticleDOI
TL;DR: Results indicate that carp adopt different strategies in an attempt to deal with short term and long term hypoxia in the natural environment.
Abstract: Biochemical and respiratory changes in the common carp Cyprinus carpio, were studied 6, 24, 96 and 168 h upon exposure to hypoxia (0·5 mgO2 l−1). Modification of kinetic properties of phosphofructokinase (PFK-1), coupled with a decreased in PFK-1 activities, were evident in muscle. No changes in kinetics and activities could be observed in muscle pyruvate kinase (PK) and lactate dehydrogenase (LDH). A decrease in muscle citrate synthase (CS) and an increase in muscle cytochrome c oxidase (CCO) were found. The common carp was able to maintain a constant level of muscle glycogen, muscle ATP, and liver CS throughout the 168-h experimental period. Changes in activities of liver LDH and muscle CCO were observed only at 168 h, which indicates that common carp may switch to alternative metabolic pathway to deal with prolonged hypoxia. A severe decrease in liver glycogen was accompanied by increases in lactate levels in both the muscle and liver. Oxygen consumption rate was reduced under hypoxia, but resumed to normoxic levels within 2 h upon return to normoxic condition. Overall, these results indicate that carp adopt different strategies in an attempt to deal with short term and long term hypoxia in the natural environment.

83 citations

Journal ArticleDOI
TL;DR: The rate of immediate myofibrillar MgADP rephosphorylation in the endogenous creatine-kinase reaction was observed to be governed by the concentration of phosphocreatine in accordance with the kinetics of this enzyme.

83 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023215
2022201
2021147
2020166
2019150
2018138