Topic
Pyruvate kinase
About: Pyruvate kinase is a research topic. Over the lifetime, 5683 publications have been published within this topic receiving 180020 citations. The topic is also known as: ATP:pyruvate 2-O-phosphotransferase & phosphoenolpyruvate kinase.
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TL;DR: The structure and location of the allosteric activator site agrees with the pattern of alternate genetic splicing of the PK gene in multicellular eukaryotes that distinguishes between a non-regulated isozyme and the regulated fetal isozymes.
417 citations
414 citations
TL;DR: Overall, data strongly suggest that the liver of most fish species is apparently capable of regulating glucose storage, and the persistent high level of endogenous glucose production independent of carbohydrate intake level may lead to a putative competition between exogenous glucose and endogenous glucose as the source of energy.
Abstract: Glucose plays a key role as energy source in the majority of mammals, but its importance in fish appears limited. Until now, the physiological basis for such apparent glucose intolerance in fish has not been fully understood. A distinct regulation of hepatic glucose utilization (glycolysis) and production (gluconeogenesis) may be advanced to explain the relative inability of fish to efficiently utilize dietary glucose. We summarize here information regarding the nutritional regulation of key enzymes involved in glycolysis (hexokinases, 6-phosphofructo-1-kinase and pyruvate kinase) and gluconeogenesis (phosphoenolpyruvate carboxykinase, fructose-1,6-bisphosphatase and glucose-6-phosphatase) pathways as well as that of the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase. The effect of dietary carbohydrate level and source on the activities and gene expression of the mentioned key enzymes is also discussed. Overall, data strongly suggest that the liver of most fish species is apparently capable of regulating glucose storage. The persistent high level of endogenous glucose production independent of carbohydrate intake level may lead to a putative competition between exogenous (dietary) glucose and endogenous glucose as the source of energy, which may explain the poor dietary carbohydrate utilization in fish.
400 citations
TL;DR: An alternative and more rapid method has been developed which is based on the following reactions: glycerol + ATP + L-glycerol 1-phosphate + ADP (glycersol kinase) ADP + phosphoenolpyruvate + NADH2 (lactate dehydrogenase)
Abstract: ENZYME methods for the assay of glycerol using glycerol dehydrogenase1 or glycerol kinase with L-glycerol 1 -phosphate dehydrogenase2 and based on the reduction of NAD to NADH2 (measured by change in extinction at 340 mµ) have the disadvantage that the reaction time is comparatively long (about 30 min). An alternative and more rapid method has been developed which is based on the following reactions: glycerol + ATP ⇌ L-glycerol 1-phosphate + ADP (glycerol kinase) ADP + phosphoenolpyruvate ⇌ ATP + pyruvate (pyruvate kinase) pyruvate + NADH2 ⇌ lactate + NAD (lactate dehydrogenase)
399 citations
TL;DR: The results strongly suggested that the fatty acid inhibition of glucose-induced l-PK transcription resulted from AMPK phosphorylation of ChREBP at Ser568, which inactivated the DNA binding activity.
391 citations