Showing papers on "RAPD published in 1900"

RAPD analysis on the germplasm resources of sunflower

Abstract: The fingerprints of 21 domestic and 11 foreign sunflower ( Helianthus annuus L.) genotypes were generated using RAPD method. Twenty_five primers were screened from 80 ten_bp arbitrary primers, and a total of 188 DNA fragments were amplified ranging from 0.26-1.98 kb, among which 164 (87.2%) were polymorphic. The average number of DNA band produced by each primer was 7.52. The result of genetic similarity analysis for 32 sunflower genotypes showed that the Nei's coefficient ranged from 0.398 7-0.853 1, and the average Nei's coefficient was 0.628?1. The Nei's coefficient of the 11 foreign sunflower genotypes ranged from 0.713 4-0.853 1, and the average Nei's coefficient was 0.782 8, suggesting that there was a close genetic relationship between them and a rich genetic polymorphism among the 21 domestic sunflower genotypes due to the Nei's coefficient (0.475 0-0.820 6) and the average Nei's coefficient (0.647 8). A DNA molecular dendrogram was established for 32 sunflower genotypes based on UPGMA cluster analysis of 188 DNA bands amplified by 25 primers, which divided the 32 sunflower genotypes into two groups: group A and group B. Group A divided into subgroups A1 and A2. A1 included 12 genotypes, viz., X10, Shaanxi sunflower, D_S 12 , Changling sunflower 6, Hei2_S 2_2 , T_C 08 , Changling sunflower 4, Baikui No.3, BH_10, J_S_B 1, Zhangyebaizikui, C 101 _S 4_3 S 4; A2 subgroup included 9 genotypes, viz., LK305_S 8, LK, CS_7, Changling sunflower S 2_S 2, Huinan7_S1_3, Huinan, CY_XX 19 _XX 2, Jikui 112, and Jikui 116. Group B was divided into subgroups B1 and B2. B1 included 10 France genotypes, viz., LG12028Q, LG9023R, CRN1435, SF9903, SF9902, S_3322, SH332, SH41, SF9001, CRN1445; B2 included only one genotype G101, which came from the USA.