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RAPD
About: RAPD is a research topic. Over the lifetime, 15960 publications have been published within this topic receiving 360391 citations.
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TL;DR: Flanking RAPD markers that are closely linked to the resistance locus in coupling phase were identified by bulked segregant analysis and represent the first essential step towards the map-based cloning of this resistance gene.
Abstract: Foliar resistance to Ascochyta lentis is controlled at a single major locus by a dominant gene (AbR
1
) in the lentil accession ILL5588 (cv ‘Northfield’). Flanking RAPD markers that are closely linked to the resistance locus in coupling phase were identified by bulked segregant analysis. Out of 261 decanucleotide primers screened 7 produced a polymorphic marker that segregated with the resistance locus, and all markers were found to exist within a single linkage group. Five of the seven RAPD markers were within 30 cM of the resistance locus. Log likelihood analysis for detecting QTL associated with the foliar resistance revealed that a single narrow peak accounted for almost 90% of the variance of resistance between the bulks. Preliminary mapping in an F3 population revealed that the closest flanking markers were approximately 6 and 14 centiMorgans (cM) away from the resistance locus. These markers should be useful for the discrimination of resistant germplasm through marker-assisted selection in future breeding programmes and represent the first essential step towards the map-based cloning of this resistance gene.
108 citations
Journal Article•
108 citations
TL;DR: The results support the hypothesis that F. oxysporum f.
108 citations
TL;DR: It is found that a molecular method like colony hybridization is suitable for screening large collections of bacteria and further characterizations by PCR and the more labourious phenotypic methods can be performed more effectively.
107 citations
TL;DR: Two resistances to downy mildew derived from Lactuca serriola were characterized genetically and mapped using molecular markers and identification of reliable PCR-based markers flanking each gene will aid in selection and in combining these resistance genes with others.
Abstract: Two resistances to downy mildew derived from Lactuca serriola were characterized genetically and mapped using molecular markers Classical genetic analysis suggested monogenic inheritance; however, the presence of multiple, tightly-linked genes in each case could not be eliminated Therefore, they were designated resistance factors R17 and R18 Analysis with molecular markers known to be linked to clusters of resistance genes quickly revealed linkage of R18 to the major cluster of resistance genes and provided six linked markers, three RAPD (Random Amplified Polymorphic DNA) markers and three codominant SCAR (Sequence Characterized Amplified Region) markers The mapping of R17 required the screening of arbitrary RAPD markers using bulked segregant analysis; this provided five linked markers, three of which segregated in the basic mapping population This demonstrated loose linkage to a second cluster of resistance genes and provided additional linked markers Two RAPD markers linked to R17 were converted into SCARs The identification of reliable PCR-based markers flanking each gene will aid in selection and in combining these resistance genes with others
107 citations