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RAPD
About: RAPD is a research topic. Over the lifetime, 15960 publications have been published within this topic receiving 360391 citations.
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TL;DR: Numerical analysis of pulsed-field gel electrophoresis patterns with endonuclease ApaI and randomly amplified polymorphic DNA (RAPD)-PCR fingerprints found RAPD-PCR appeared to be a suitable method for typing and monitoring O. oeni strains in winemaking.
Abstract: Genetic diversity of 60 Oenococcus oeni strains from different wines was evaluated by numerical analysis of (i) pulsed-field gel electrophoresis (PFGE) patterns with endonuclease ApaI and (ii) randomly amplified polymorphic DNA (RAPD)-PCR fingerprints with four oligonucleotide primers. Sixty-two percent of the strains could be distinguished by PFGE, whereas most strains were identified by distinct RAPD-PCR profiles and associated according to the geographical origin. Because of its rapidity and reliability, RAPD-PCR appeared to be a suitable method for typing and monitoring O. oeni strains in winemaking.
99 citations
TL;DR: The level of genetic diversity of Salvia fruticosa in Crete and its relation to the essential oil profiles was assessed using RAPD markers.
99 citations
TL;DR: Random amplified polymorphic DNA (RAPD) analysis was evaluated for the selection and elimination of bacterial strains used in microbial screening and was a very useful tool for excluding similar bacterial isolates encountered during screening.
Abstract: For efficient fungal strain selection in microbial screening, we applied the random amplified polymorphic DNA (RAPD) method using the polymerase chain reaction (PCR). In order to evaluate this system, the genus Trichoderma was employed, because its species are difficult to distinguish from each other. We selected an appropriate oligonucleotide decamer, R28 (5'-ATGGATCCGC), determined the optimal cycles of PCR as 30 cycles, simplified the template preparation method, and determined optimal concentrations of the template and Taq DNA polymerase. We then examined 74 closely related strains of Trichoderma. The electrophoretic band patterns of the PCR products were compared. According to the statistical analysis with the phylogenetic analysis using parsimony (PAUP), the results were consistent with the morphological, physiological and ecological data on these strains. Therefore, we conclude that RAPD is a simple, efficient and reliable method for the selection of fungal strains employed in screening.
99 citations
TL;DR: The specific clustering of the highly diverse group of Vasconcella × heilbornii accessions suggests that these genotypes may be the result of bi-directional introgression events between Vascon cella stipulata and Vascon Cella cundinamarcensis.
Abstract: The AFLP technique was used to assess the genetic relationships among the cultivated papaya (Carica papaya L) and related species native to Ecuador Genetic distances based on AFLP data were estimated for 95 accessions belonging to three genera including C papaya, at least eight Vasconcella species and two Jacaratia species Cluster analysis using different methods and principal co-ordinate analysis (PCO), based on the AFLP data from 496 polymorphic bands generated with five primer combinations, was performed The resulted grouping of accessions of each species corresponds largely with their taxonomic classifications and were found to be consistent with other studies based on RAPD, isozyme and cpDNA data The AFLP analysis supports the recent rehabilitation of the Vasconcella group as a genus; until recently Vasconcella was considered as a section within the genus Carica Both cluster and PCO analysis clearly separated the species of the three genera and illustrated the large genetic distance between C papaya accessions and the Vasconcella group The specific clustering of the highly diverse group of Vasconcella × heilbornii accessions also suggests that these genotypes may be the result of bi-directional introgression events between Vasconcella stipulata and Vasconcella cundinamarcensis
99 citations
TL;DR: The high degree of population differentiation recorded here highlights the need for additional conservation measures for this species, both in terms of incorporating further populations into the protected areas, and the restoration of severely degraded populations, to ensure their continued viability.
99 citations