RAPD

About: RAPD is a research topic. Over the lifetime, 15960 publications have been published within this topic receiving 360391 citations.

Genetic structure in wild populations of black tiger shrimp (Penaeus monodon) using randomly amplified polymorphic DNA analysis.

Abstract: Randomly amplified polymorphic DNA (RAPD) analysis was used to examine genetic variation in wild black tiger shrimp, Penaeus monodon Specimens were collected from five geographically separated locations (Satun-Trang, Phangnga, and Medan in the Andaman Sea and Chumphon and Trad in the Gulf of Thailand) A total of 100 P monodon individuals were investigated using seven arbitrarily selected primers Fifty-eight (725%) of eighty reproducible RAPD fragments ranging in size from 200 to 2200 bp were polymorphic The percentages of polymorphic bands of the five geographic populations investigated varied from 515 to 577% The genetic distance between populations and UPGMA dendrograms indicated that the Medan population was genetically different from Thai P monodon (Dij = 14976%) Within Thailand, the Satun-Trang P monodon was separated from the remaining geographic populations with a genetic distance of 2632% RAPD analysis in the present study yielded a total of 252 genotypes A Monte Carlo analysis illustrated geographic heterogeneity in genotype frequencies within this species, suggesting that genetic population structure does exist in this taxon (P < 0001 for all primers) Signficant differences in genotype frequencies between Thai and Indonesian (Medan) P monodon were observed (P < 00001) Within Thailand, the Andaman Sea P monodon was significantly different from that of the Gulf of Thailand (P values between 00000 and 00387), indicating population differentiation between P monodon from these two main fishery regions of Thailand

Development of a sequence characterized amplified region (SCAR) based indirect selection method for a dominant blast-resistance gene in rice

Abstract: Two randomly amplified polymorphic DNA (RAPD) markers, OPF62700 and OPH182400, tightly linked to Pi-10, a dominant blast-resistance gene conferring complete resistance to isolate 106 (international...

Choice of methodology for assessing genetic impacts of environmental stressors: polymorphism and reproducibility of RAPD and AFLP fingerprints.

Abstract: PCR-based multi-locus DNA fingerprints represent one of the most informative and cost-effective measures of genetic diversity and are useful population-level biomarkers of toxicologic and other anthropogenic impacts. However, concerns about reproducibility of DNA fingerprints have limited their wider use in environmental biology. We assessed polymorphism and reproducibility of two common fingerprinting techniques, RAPD (randomly amplified polymorphic DNA) and AFLP (amplified fragment length polymorphism), in pedigreed populations of rainbow trout (Oncorhynchus mykiss) to derive general rules for selective removal of problematic fingerprint bands. We found that by excluding bands that comprised less than 1% of total intensity, and by excluding the largest and smallest 10% of the bands, we could achieve nearly 100% reproducibility of AFLP fingerprints. Similar application of band exclusion criteria to RAPD fingerprints did not significantly enhance their reproducibility, and at least 15% of RAPD bands were not fully repeatable, heritable, or transmittable. The RAPD technique produced more polymorphic fingerprints than AFLP; however, considering that a substantial proportion of RAPD markers did not demonstrate Mendelian inheritance patterns, the AFLP methodology is to be preferred for future research.

Genetic diversity in Elymus caninus as revealed by isozyme, RAPD, and microsatellite markers.

Abstract: Genetic diversity of 33 Elymus caninus accessions was investigated using isozyme, RAPD, and microsatellite markers. The three assays differed in the amount of polymorphism detected. Microsatellites...