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RAPD

About: RAPD is a research topic. Over the lifetime, 15960 publications have been published within this topic receiving 360391 citations.


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Journal ArticleDOI
TL;DR: Eleven of the most promising Sarilop clones and one clone of Sarizeybek, all selected from a former agronomic evaluation, were analysed by three molecular marker techniques, isozymes, RAPDs and AFLPs and the resolution power and the accuracy of these three analytical techniques, in distinguishing among fig clones were determined.

89 citations

Journal ArticleDOI
TL;DR: This new procedure, called two primers (TP)‐RAPD fingerprinting, is rapid, sensitive, reliable, highly reproducible and suitable for experiments with a large number of microorganisms, and can be applied to bacterial taxonomy, ecological studies and for the detection of new bacterial species.
Abstract: Polymerase chain reation (PCR) fingerprints are used to characterize and recognize bacteria and are generally obtained using universal primers that generate an array of DNA amplicons, which can be separated by electrophoresis Universal primers 8F and 1491 R have been used to amplify specifically 16S rDNA We have used these primers at an annealing temperature of 50 degrees C Agarose gel electrophoresis of PCR products revealed several bands The band pattern of each bacterial species was different and the strains belonging to the same species shared an identical pattern The patterns obtained did not show variations with plasmid DNA content or the growth stage of the bacteria The peculiarity of the randomly amplified polymorphic DNA (RAPD) described in this work lies in the use of two large primers (proximately 20 nt) to obtain the pattern, since normally a only smaller primer is used, and in the new application for the primers used to amplify 16S rDNA This new procedure, called two primers (TP)-RAPD fingerprinting, is thus rapid, sensitive, reliable, highly reproducible and suitable for experiments with a large number of microorganisms, and can be applied to bacterial taxonomy, ecological studies and for the detection of new bacterial species

89 citations

Journal ArticleDOI
01 Aug 1996-Genetics
TL;DR: SSCP analysis of RAPD-PCR markers offers a rapid and inexpensive means of constructing intensive linkage maps of many species, and uses silver staining to detect DNA fractionated on large thin polyacrylamide gels and reveals more polymorphic markers than agarose gel electrophoresis.
Abstract: The use of random amplified polymorphic DNA from the polymerase chain reaction (RAPD-PCR) allows efficient construction of saturated linkage maps. However, when analyzed by agarose gel electrophoresis, most RAPD-PCR markers segregate as dominant alleles, reducing the amount of linkage information obtained. We describe the use of single strand conformation polymorphism (SSCP) analysis of RAPD markers to generate linkage maps in a haplodiploid parasitic wasp Bracon (Habrobracon) hebetor and a diploid mosquito, Aedes aegypti. RAPD-SSCP analysis revealed segregation of codominant alleles at markers that appeared to segregate as dominant (band presence/band absence) markers or appeared invariant on agarose gels. Our SSCP protocol uses silver staining to detect DNA fractionated on large thin polyacrylamide gels and reveals more polymorphic markers than agarose gel electrophoresis. In B. hebetor, 79 markers were mapped with 12 RAPD primers in six weeks; in A. aegypti, 94 markers were mapped with 10 RAPD primers in five weeks. Forty-five percent of markers segregated as codominant loci in B. hebetor, while 11% segregated as codominant loci in A. aegypti. SSCP analysis of RAPD-PCR markers offers a rapid and inexpensive means of constructing intensive linkage maps of many species.

89 citations

Journal ArticleDOI
TL;DR: The data provide a basis for the isolation of the RPP5 locus by positional cloning as a first step towards understanding recognitional specificity in plant-pathogen interactions at a molecular level.
Abstract: Peronospora parasitica causes downy mildew on crucifers. An isolate of P. parasitica (denoted NoCO2) was identified that infected Arabidopsis plants of the land race Columbia (Col-0) but not plants of land race Landsberg erecta (La-er). Segregation analysis of F2 plants derived from a La-er x Col-0 cross established that the resistance was inherited as a single locus, denoted RPP5. Macroscopic and microscopic examinations of inoculated La-er and Col-0 cotyledons showed that restriction of fungal growth in La-er was accompanied by massive callose accumulation and death of plant cells in direct contact with points of attempted fungal penetration. La-er x Col-0 F1 plants exhibited an intermediate resistance response in all aspects of fungal development, indicating that RPP5 is semi-dominant in its action. F8 recombinant inbred lines generated between La-er and Col-0 were used to map RPP5 to a narrow interval (<1.1 cM) on chromosome 4, utilizing existing restriction fragment length polymorphic (RFLP) markers and newly generated random amplified polymorphic DNA (RAPD) markers. The data provide a basis for the isolation of the RPP5 locus by positional cloning as a first step towards understanding recognitional specificity in plant-pathogen interactions at a molecular level.

89 citations

Journal ArticleDOI
01 Jun 1994-Genome
TL;DR: A direct comparison of the ability of four selected random amplified polymorphic DNA (RAPD) primers and a GACA-containing microsatellite probe to detect genetic variation in Lycopersicon indicates that the two methods detect two types of DNA that differ in their degree of variability.
Abstract: In this study, a direct comparison was made of the ability of four selected random amplified polymorphic DNA (RAPD) primers and a GACA-containing microsatellite probe to detect genetic variation in Lycopersicon. Of the 89 RAPD primers initially tested, 85 showed differences between a representative of Lycopersicon pennellii and L. esculentum, but only 4 distinguished among three L. esculentum cultivars. These four primers were subsequently tested on representatives of six Lycopersicon species. In pairwise comparisons of species, all or 14 of the 15 combinations could be distinguished by single primers. When the primers were tested on 15 L. esculentum cultivars, 90 of the 105 combinations could be distinguished by the four primers together. Finally, none of 118 tested primers showed reproducible differences among calli or progeny of regenerants from tissue culture, although some of the plants had inherited morphological mutations. The probe pWVA16, which detects GACA-containing microsatellites, could distinguish in TaqI-digested DNA the representatives of Lycopersicon species as well as all the L. esculentum cultivars tested. The probe was unable to detect polymorphisms among calli and the progeny of regenerants from tissue culture. An analysis of the results showed that the four selected RAPD primers were able to detect polymorphic bands among species at a frequency of 80%, and among cultivars at a frequency of 44%. In contrast, the microsatellite probe detected polymorphic bands at a frequency of 100 and 95%, respectively. The GACA-containing probe did not detect any common bands among the representatives of the six species, while band sharing with RAPDs was 48%. These results indicate that the two methods detect two types of DNA that differ in their degree of variability.

88 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20241
2023149
2022309
2021152
2020195
2019246