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RAPD
About: RAPD is a research topic. Over the lifetime, 15960 publications have been published within this topic receiving 360391 citations.
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TL;DR: Two molecular techniques which reveal highly variable DNA polymorphisms were used to evaluate genetic diversity between six aquacultural strains of Oreochromis niloticus (tilapia) from the Philippines, and RAPD was technically easier to perform and produced results with low statistical error, whereas DNA fingerprinting detected greater genetic differentiation between strains.
Abstract: Two molecular techniques which reveal highly variable DNA polymorphisms, RAPD and multilocus DNA fingerprinting, were used to evaluate genetic diversity between six aquacultural strains of Oreochromis niloticus (tilapia) from the Philippines. The results using both techniques were in close agreement Within-strain heterozygosity values were similar and were correlated between the two data sets, but statistical errors associated with the RAPD data set were lower. Although genetic distances between strains were greater using DNA fingerprinting, the distances measured using both methods were significantly correlated. Both methods were useful in estimating variation between strains, but they offered different advantages. RAPD was technically easier to perform and produced results with low statistical error, whereas DNA fingerprinting detected greater genetic differentiation between strains. The theoretical basis for using RAPD and multilocus minisatellite markers for population studies is discussed.
88 citations
TL;DR: Interspecific hybridization among Hawaiian species of Cyrtandra (Gesneriaceae) was investigated using randomly amplified polymorphic DNA (RAPD) markers, suggesting that these interspecific hybridization events had, and continue to have, an effect on the adaptive radiation and conservation biology ofyrtandra.
Abstract: Interspecific hybridization among Hawaiian species ofCyrtandra (Gesneriaceae) was investigated using randomly amplified polymorphic DNA (RAPD) markers. Thirty-three different primers were used to investigate interspecific hybridization for 17 different putative hybrids based on morphological intermediacy and sympatry with putative parental species. RAPD data provided evidence for the hybrid origin of all putative hybrid taxa examined in this analysis. However, the patterns in the hybrid taxa were not found to be completely additive of the patterns found in the parental species. Markers missing in the hybrid taxa can be attributed to polymorphism in the populations of the parental species and the dominant nature of inheritance for RAPD markers. Unique markers found within hybrid taxa require further explanation but do not necessarily indicate that the taxa are not of hybrid origin. The implications suggest that these interspecific hybridization events had, and continue to have, an effect on the adaptive radiation and conservation biology ofCyrtandra.
88 citations
TL;DR: This work investigated the efficiency of marker-assisted selection with RAPD markers linked in coupling and in repulsion with a single resistance allele to investigate the effectiveness of indirect selection and pyramiding of monogenic pest resistance traits in crop species.
Abstract: The random amplified polymorphic DNA (RAPD) assay has recently provided a marker system that may be useful for indirect selection and pyramiding of monogenic pest resistance traits in crop species. Our objective was to investigate the efficiency of marker-assisted selection with RAPD markers linked in coupling and in repulsion with a single resistance allele. Common bean (Phaseolus vulgaris L.) near-isogenic lines differing for the recessive bean common mosaic virus (BCMV) resistance allele bc-3 were screened to identify linked RAPD markers [...]
88 citations
TL;DR: The random amplified polymorphic DNA (RAPD) method was investigated as a potential fish species identification method and profiles generated were consistent within this group of fish.
88 citations
TL;DR: RAPDs appear to be as efficient as RFLPs for classifying cocoa genotypes, and the nature of RAPD bands was investigated by Southern analysis.
Abstract: The genetic diversity of 106 genotypes of Theobroma cacao L. was assessed using RFLP and RAPD methods. Thirty-one cDNA probes and 19 primers generated, respectively, 87 and 49 polymorphic bands that were submitted to factorial and cluster analyses. Both RFLP and RAPD bands separated the individuals into three distinctive groups. Some genotypes did not correspond to the currently accepted classification and are considered to have been originally misclassified. The nature of RAPD bands was investigated by Southern analysis. There were as many highly repeated sequences as single copy sequences but the two types of sequences did not structure the variability in the same way. Similar results were obtained with RFLPs and RAPDs with regard to the genetic structure of Theobroma cacao L. Therefore, RAPDs appear to be as efficient as RFLPs for classifying cocoa genotypes.
88 citations