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RAPD
About: RAPD is a research topic. Over the lifetime, 15960 publications have been published within this topic receiving 360391 citations.
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TL;DR: The microbial diversity within Alberquilla cheese, made from a spontaneously fermented mixture of raw goats' and sheep's milk in the Alpujarra mountains, has been studied by the classical culturing method and also by molecular analysis of community DNA.
85 citations
TL;DR: Gynogenesis of four onion cultivars was induced, starting from ovules and ovaries, using a 2-step culture procedure and revealed several missing bands compared with donor plants, but micropropagated clones of different ploidy level were identical among each other.
84 citations
TL;DR: The results were highly significant in that they indicated a restricted population differentiation, as would be expected from outcrossing species, and there was significant variation among populations in terms of mean seed number per flower and mean seed mass per population.
Abstract: It is widely assumed that population size significantly affects the dynamics of plant populations. Smaller populations are threatened by genetic drift and inbreeding depression, both of which may result in a decrease of genetic variation and a resulting negative impact on plant fitness. In our study we analysed the patterns of random amplified polymorphic DNA (RAPD) variation among 10 Dictamnus albuspopulations of varying size. The aim was to examine local differentiation in relation to spatial isolation resulting from limited population size and geographical distancing between populations. Significant correlations were noted between population size and both percentage of polymorphic loci (P < 0.01) and genetic diversity (P < 0.01). The matrix correlation between genetic and geographical distances revealed that geographical differentiation was reflected in the RAPD profile (Mantel test: r2=0.34, P < 0.001). We found the highest level of molecular variance of RAPD patterns among individuals within the populations (72.6%), whereas among-population variation accounted for only 21.6% of variation. These results were highly significant in that they indicated a restricted population differentiation, as would be expected from outcrossing species. An additional analysis of seed production showed that there was significant variation among populations in terms of mean seed number per flower and mean seed mass per population which could be attributed to differences in population size as well as levels of genetic variation.
84 citations
TL;DR: Mixed pollinations in experimental and natural conditions revealed hybridization rates for individuals of different populations of the wild species differed considerably: 1% for two populations known for more than 90 years versus 7% for a newly discovered population.
Abstract: In order to estimate the potential of gene flow between wheat (Triticum aestivum L.) and jointed goatgrass (Aegilops cylindrica Host.), we carried out mixed pollinations in experimental and natural conditions. A set of species-specific RAPD (random amplified polymorphic DNA) and microsatellite markers were used to detect the presence of parental markers in the progeny of the plants used in these experiments. No hybrids were found within the offsprings of the plants used for the greenhouse experiments, while 85 Ae. cylindrica×T. aestivum hybrids were found within 2400 analyzed F1 plants resulting from the field pollinations. The hybridization rates for individuals of different populations of the wild species differed considerably: 1% for two populations known for more than 90 years versus 7% for a newly discovered population. Most of the hybrids were completely sterile, but five of them produced 13 seeds (BC1) by backcross with Ae. cylindrica. Twelve seeds germinated and generated viable and partly fertile plants. About 25% of the wheat specific RAPD markers were found in the BC1 plants, indicating that introgression of wheat DNA into Ae. cylindrica is possible. In addition, one microsatellite marker, known to be situated on the D genome (a genome shared by both species), was also found in the BC1 plants.
84 citations
TL;DR: Analysis of molecular variance revealed that 94.7% of the genetic diversity in mango existed within regions, however, differences among regions were significant; northern and eastern regions formed one zone and western and southern regions formed another zone of mango diversity in India.
Abstract: SummaryRandom amplified polymorphic DNA analysis was carried out in 29 Indian mango cultivars comprising popular landraces and some advanced cultivars. PCR amplification with 24 primers generated 314 bands, 91.4% of which were polymorphic. Jaccard’s similarity between pairs of cultivars ranged between 0.318 and 0.75 with a mean of 0.565. A UPGMA dendrogram showed the majority of the cultivars from northern and eastern regions of India clustering together and separate from southern and western cultivars. Analysis of molecular variance revealed that 94.7% of the genetic diversity in mango existed within regions. However, differences among regions were significant; northern and eastern regions formed one zone and western and southern regions formed another zone of mango diversity in India.
84 citations