Topic
RAPD
About: RAPD is a research topic. Over the lifetime, 15960 publications have been published within this topic receiving 360391 citations.
Papers published on a yearly basis
Papers
More filters
TL;DR: Analysis of genetic structure of the populations of the blacklip abalone Haliotis rubra of Victoria, Australia revealed that a Point Cook population sampled from within the semi-enclosed Port Phillip Bay was distinct from two other central zone populations.
Abstract: We investigated the utility of three polymerase chain-reaction (PCR)-based DNA molecular markers in analysing genetic structure of the populations of the blacklip abalone Haliotis rubra (Leach) of Victoria, Australia The DNA markers included 84 randomly amplified polymorphic DNA (RAPD) bands amplified using six random primers, two minisatellites, GHR (putative growth-hormone-gene-repeat) and MIPR (putative mollusca-insulin-like peptide-gene-repeat), and three microsatellites, RUBGT1 [containing (GT)n repeats], RUBCA1 [containing (CA)n repeats] and RUBGACA1 [containing (GACA)n repeats] All three types of DNA markers revealed significant subdivision in the H rubra populations along the coastline This is postulated as being related to the abalone's relatively short pelagic period and limited dispersion Further analysis revealed that a Point Cook population sampled from within the semi-enclosed Port Phillip Bay was distinct from two other central zone populations (Apollo Bay and Cape Schanck) The genotypes of microsatellites indicated excessive homozygotes across all the populations at all three microsatellite loci, and possible causes such as larval recruitment pattern and asynchronous spawning are discussed The excessive homozygotes recorded for the three microsatellite loci contrast with those observed in the minisatellite loci GHR and MIPR, the heterozygosities of which were at Hardy–Weinberg equilibrium
115 citations
TL;DR: Ten loci linked with the gene for resistance to white pine blister rust were identified and segregation data was obtained from five families, and these and other markers derived by this approach may provide starting points for map-based cloning of this important gene.
Abstract: We have genetically mapped a gene for resistance to white pine blister rust (Cronartium ribicola Fisch.) in sugar pine (Pinus lambertiana Dougl.) by using an approach which relies on three factors: (i) the ability to assay for genetic markers in the haploid stage of the host's life cycle, using megagametophyte seed tissue; (ii) a simple and clearly defined pathosystem; and (iii) the use of random amplified polymorphic DNA (RAPD) markers that can be quickly and efficiently evaluated. Resistance to white pine blister rust in sugar pine is known to be controlled by a single dominant gene (R). Maternal segregation of R and dominant RAPD markers were scored simultaneously following collection of megagametophytes for DNA assays and seedling inoculation with C. ribicola. Bulked samples of haploid megagametophyte DNA from resistant and susceptible offspring of segregating full-sib and half-sib families were used to evaluate 800 random decanucleotide primers. Ten loci linked with the gene for resistance to white pine blister rust were identified and segregation data were obtained from five families. Six of the linked markers were within 5 centimorgans of the gene, and one marker was 0.9 centimorgan from R. These and other markers derived by this approach may provide starting points for map-based cloning of this important gene.
115 citations
TL;DR: Analysis of the variability detected using RAPDs clearly discriminated between the geographical origin of the three cocoa populations and revealed that most variation was detected within a population.
Abstract: In order to preserve and exploit the valuable genetic resources of tropical forest trees, such as cocoa, a systematic assessment of the available genetic variability is necessary. The approach we have used is based on a simple mini-prep DNA extraction procedure together with a polymerase-chain-reaction- (PCR)-based polymorphic assay procedure (RAPD). Twenty-five cocoa accessions: IMCs and PAs collected from Peru and LCTEENs collected from Ecuador, which are difficult to distinguish using morphological or biochemical descriptors, were uniquely fingerprinted using a minimum of three oligonucleotide primers. Analysis of the variability detected using RAPDs clearly discriminated between the geographical origin of the three cocoa populations. Partitioning of variability into within and between population components revealed that most variation was detected within a population. The potential of RAPD analysis to facilitate the rationalization of field gene banks and provide accurate estimates of diversity to allow optimization of collecting strategies is discussed
115 citations
TL;DR: Two SCAR markers, SCAR1265 and SCAR1400, were developed to detect the Pm21 gene in different genetic backgrounds and enable large-scale accurate screening for the presence/absence of Pm 21 allele.
Abstract: Powdery mildew is an important disease in most of the wheat production areas of the world. The resistance gene Pm21 (6AL/6VS trans-location) derived from Haynaldia villosa confers resistance to all available isolates of Erysiphe (Blumeria) graminis f. sp. tritici in China and Europe. The objective of this study was to develop fast and reliable sequence characterized amplified region (SCAR) markers linked to the Pm21 gene. A random amplified polymorphic DNA (RAPD) marker for Pm21, OPH171400, was converted to SCAR markers after sequencing the two ends of the polymorphic DNA fragment. Two SCAR markers, SCAR1265 and SCAR1400, were developed to detect the Pm21 gene in different genetic backgrounds. The specific SCAR1265 marker enable large-scale accurate screening for the presence/absence of Pm21 allele.
115 citations
TL;DR: This study identified genetic loci associated with 14 quantitative traits responsible for seed yield, yield components, and plant architecture traits in common bean to improve understanding of the genetic factors conditioning these traits and is expected to assist in the selection of superior genotypes.
Abstract: SBreeding effort to improve yield and other quantitative traits in common bean (Phaseolus vulgaris L.) has proven to be difficult. The use of molecular markers will improve our understanding of the genetic factors conditioning these traits and is expected to assist in the selection of superior genotypes. This study was conducted to identify genetic loci associated with 14 quantitative traits responsible for seed yield, yield components, and plant architecture traits in common bean. A population of 142 F(2:4) lines that was developed from a cross between OAC Seaforth and OAC 95-4, was evaluated at two locations in Canada in 1998. The lines were assayed for random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP), simple sequence repeat (SSR), and amplified fragment length polymorphism (AFLP) markers. One hundred fourteen markers were assigned to 12 linkage groups. Growth habit and resistance to common bacterial blight [CBB, caused by Xanthomonas axonopodis pv. phaseoli (Smith) Vauterin, Hoste, Kosters & Swings = syn. X. campestris pv. phaseoli (Smith) Dye] were each mapped as single major genes on linkage groups G11 and G5, respectively. An alignment of the current map with the previous linkage map developed at the University of Florida and the core linkage map of bean was produced from 30 RFLP loci. Twenty quantitative trait loci (QTL) were identified for the 14 traits that were analyzed. The number of QTL identified per trait ranged from one to three. A multiple QTL model for each trait showed that these genomic regions accounted for 11.3 to 43.1% of the total phenotypic variation for the traits. Five of the twenty QTL were detected at both locations. The strengths of QTL effects for a given trait appeared to be slightly different among locations, but the positions of QTL on the map were stable across locations.
115 citations