Topic
Rapeseed
About: Rapeseed is a research topic. Over the lifetime, 2945 publications have been published within this topic receiving 51790 citations. The topic is also known as: Brassica napus & rape.
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TL;DR: Two new proteins were identified in this study, one of which is similar to a triacylglycerol‐associated factor from narrow‐leafed lupin while the other, a protein annotated as a myrosinase associated protein, shows high similarity to the lipase/hydrolase family of enzymes with GDSL‐motifs.
Abstract: Oil bodies were purified from mature seed of two Brassica napus crop cultivars, Reston and Westar. Purified oil body proteins were subjected to both 2-DE followed by LC-MS/MS and multidimensional protein identification technology. Besides previously known oil body proteins oleosin, putative embryo specific protein ATS1, (similar to caleosin), and 11-beta-hydroxysteroid dehydrogenase-like protein (steroleosin), several new proteins were identified in this study. One of the identified proteins, a short chain dehydrogenase/reductase, is similar to a triacylglycerol-associated factor from narrow-leafed lupin while the other, a protein annotated as a myrosinase associated protein, shows high similarity to the lipase/hydrolase family of enzymes with GDSL-motifs. These similarities suggest these two proteins could be involved in oil body degradation. Detailed analysis of the two other oil body components, polar lipids (lipid monolayer) and neutral lipids (triacylglycerol matrix) was also performed. Major differences were observed in the fatty acid composition of polar lipid fractions between the two B. napus cultivars. Neutral lipid composition confirmed erucic acid and oleic acid accumulation in Reston and Westar seed oil, respectively.
142 citations
TL;DR: Research advances in Arabidopsis molecular genetics, and the emerging Brassica-Arabidopsis genome relatedness, will enable the radical manipulation of many key agronomic traits in rapeseed, ranging from greatly improved seed-oil yield and quality, to improved disease resistance, pod shattering and canopy architecture.
133 citations
TL;DR: Environmental factors can greatly influence the outcrossing rate in rapeseed, and therefore changes in the heterozygosity level of a variety may occur during multiplication, merit further studies.
Abstract: Rapeseed (Brassica napus) is a predominantly selfpollinated crop with about one-third outcrossing. The outcrossing rate may be influenced by environmental factors, and hence changes in the heterozygosity level of a variety may occur during multiplication. In an investigation on environmental variation in outcrossing, we estimated the outcrossing rate in the Swedish spring rapeseed cv 'Topas' by isozyme analysis and found that outcrossing varied from 12% to 47% over five locations in Sweden, Denmark and Germany. Among flowers at different positions on the same plant, average outcrossing varied from 11% at the top to 39% at the bottom of the plant. In conclusion, environmental factors can greatly influence the outcrossing rate in rapeseed, and an investigation therefore merit further studies.
132 citations
TL;DR: Current goals include the breeding of yellow-seeded rapeseed lines with high content of seed oil and the exploitation of Arabidopsis knowledge will be presented and discussed.
132 citations