About: Reagent is a(n) research topic. Over the lifetime, 60091 publication(s) have been published within this topic receiving 1234928 citation(s). The topic is also known as: reagens.
Papers published on a yearly basis
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Abstract: Since 1922 when Wu proposed the use of the Folin phenol reagent for the measurement of proteins, a number of modified analytical procedures utilizing this reagent have been reported for the determination of proteins in serum, in antigen-antibody precipitates, and in insulin. Although the reagent would seem to be recommended by its great sensitivity and the simplicity of procedure possible with its use, it has not found great favor for general biochemical purposes. In the belief that this reagent, nevertheless, has considerable merit for certain application, but that its peculiarities and limitations need to be understood for its fullest exploitation, it has been studied with regard to effects of variations in pH, time of reaction, and concentration of reactants, permissible levels of reagents commonly used in handling proteins, and interfering substances. Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
01 Mar 1959-Analytical Chemistry
26 May 1987-Analytical Biochemistry
TL;DR: This new method maintains the high sensitivity and low protein-to-protein variation associated with the Lowry technique and demonstrates a greater tolerance of the bicinchoninate reagent toward such commonly encountered interferences as nonionic detergents and simple buffer salts.
Abstract: Cu + produced during the reaction of protein with alkaline Cu ++ can be monitored by measuring the absorbance at 562 nm of the intense purple complex formed with the ion of bicinchoninic acid (BCA). The color produced is stable and increases in a linear fashion over a broad working range of increasing protein concentration. Since BCA is stable, it is incorporated in the reagent formulation at the start of the reaction. Thus, the method offers mechanical simplification over the method described by Lowry et al.
TL;DR: In this paper, the use of Folin-Ciocalteu reagent rather than the FolinDenis reagent, gallic acid as a reference standard, and a more reproducible time-temperature color development period was investigated.
Abstract: Several details of the assay of total phenolic substances have been investigated and an improved procedure developed. The improvements include the use of Folin-Ciocalteu reagent rather than the Folin-Denis reagent, gallic acid as a reference standard, and a more reproducible time-temperature color development period. The values obtained are less subject to variation and interference from several nonphenols, yet are directly comparable to the "tannin" values obtained by the previously standard method.
01 Jan 1962-Analytica Chimica Acta
TL;DR: In this article, a single solution reagent was described for the determination of phosphorus in sea water, which consists of an acidified solution of ammonium molybdate containing ascorbic acid and a small amount of antimony.
Abstract: A single solution reagent is described for the determination of phosphorus in sea water. It consists of an acidified solution of ammonium molybdate containing ascorbic acid and a small amount of antimony. This reagent reacts rapidly with phosphate ion yielding a blue-purple compound which contains antimony and phosphorus in a 1:1 atomic ratio. The complex is very stable and obeys Beer's law up to a phosphate concentration of at least 2 μg/ml.The sensitivity of the procedure is comparable with that of the stannous chloride method. The salt error is less than 1 %.
Trending Questions (10)