Reagent

About: Reagent is a research topic. Over the lifetime, 60091 publications have been published within this topic receiving 1234928 citations. The topic is also known as: reagens.

Preparation of the Reagent o-Nitrobenzenesulfonylhydrazide.

Abstract: o-Nitrobenzenesulfonylhydrazide (NBSH) has been shown to be a valuable reagent for the synthesis of allenes from propargylic alcohols, for the reductive transposition of allylic alcohols, and, most recently, for the deoxygenation of unhindered alcohols. Each of these transformations proceeds by Mitsunobu displacement of an alcohol with NBSH followed by in situ elimination of o-nitrobenzenesulfinic acid to form a monoalkyl diazene intermediate. The mild reaction conditions (neutral pH, reaction temperatures ≤23 °C) are especially attractive for substrates containing sensitive functional groups. The success of this methodology relies critically upon the purity of the NBSH reagent employed in the reaction. A simple and practical procedure for the preparation of NBSH of high purity and guidelines for the handling and storage of the reagent are provided in this note.

Multizone analytical element having labeled reagent concentration zone

Abstract: A multizone test device for the determination of analyte from a liquid test medium upon contact with the liquid test medium and a labeled reagent comprising a chemical group having a detectable physical property. The test device preferably comprises multilayers including a reagent layer incorporated with an immobilized reagent and a detection layer incorporated with an immobilized form of a binding substance for the labeled reagent. The immobilized reagent and the labeled reagent comprise specific binding partners which will bind to each other dependent upon the amount of analyte present. Labeled reagent which does not become bound to the immobilized reagent in the reagent layer migrates into the detection layer and becomes bound to and immobilized by the immobilized binding substance therein. As a result, reverse migration of the labeled reagent into the reagent layer is prevented and the detectable physical property provided by the label of the labeled reagent is localized in the detection layer for the precise measurement thereof and correlation to the amount of analyte in the test medium.