Topic
Reagent
About: Reagent is a research topic. Over the lifetime, 60091 publications have been published within this topic receiving 1234928 citations. The topic is also known as: reagens.
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TL;DR: In this paper, the standard method for the colour development proposed is based on an investigation of the following variables: hydrogen sulphide losses, acidity at reaction and measurement, reagent concentrations, temperature at reaction, concentration of traces of heavy metals, time, and air-oxidation.
135 citations
TL;DR: Practical methods for both extraction of nucleotide sugars from cell lysates and for their analytical separation enable good separation of structurally similar sugar nucleotides, compatibility with rapid evaporative concentration, and possibility to automation.
Abstract: Analysis of nucleotide sugar metabolism is essential in studying glycosylation in cells. Here we describe practical methods for both extraction of nucleotide sugars from cell lysates and for their analytical separation. Solid-phase extraction cartridges containing graphitized carbon can be used for the purification of nucleotide sugars by using triethylammonium acetate buffer as a ion-pairing reagent for decreasing retention. After that they are separated by high-performance liquid chromatography using a C18 reversed-phase column and the same ion-pairing reagent for increasing retention. These new sample preparation and analysis methods enable good separation of structurally similar sugar nucleotides, compatibility with rapid evaporative concentration, and possibility to automation. Monitoring the production of GDP-deoxyhexoses in genetically engineered yeast and native bacterial cells are described here as specific applications.
134 citations
134 citations
TL;DR: This work demonstrates that the integrated process combining SBR with Fenton's reagent could provide comprehensive treatment to swine wastewater and helped to not only remove total organic carbon, heavy metals, and total phosphorus, but also inactivate bacteria and reduce wastewater toxicity.
134 citations
TL;DR: In this paper, a spectrophotometric sequential injection (SI) determination of phosphate and silicate in environmental samples and cell cultivation medium using the molybdenum blue reaction was presented.
134 citations