Topic
Reagent
About: Reagent is a research topic. Over the lifetime, 60091 publications have been published within this topic receiving 1234928 citations. The topic is also known as: reagens.
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TL;DR: BH 3 Bu 2 OTf is an effective reagent to reductively cleave 4,6-O-benzylidene acetals of various hexopyranosides to the corresponding 4-O-, O-, Isopropyl-idene ethers.
131 citations
TL;DR: These authors demonstrated that the Rydon and Smith technique is superior to the ninhydrin technique for the detection of cyclic peptides, acylated peptides and amino acids, proteins, and high molecular weight peptides.
131 citations
TL;DR: The results indicate that intramolecular protein cleavage reactions mediated by EDTA-Fe can be used to evaluate changes in protein conformation and should be a useful tool in the structural mapping of nonnative protein states populated at equilibrium, such as the molten globule.
Abstract: We report the synthesis and evaluation of (EDTA-2-aminoethyl) 2-pyridyl disulfide. By using this easily prepared cysteine-specific hydrophilic reagent, an ethylenediaminetriacetic acid-Fe3+ complex (EDTA-Fe) was covalently attached to a single genetically engineered cysteine residue in staphylococcal nuclease. Upon addition of the iron reductant ascorbate, the nuclease-EDTA-Fe conjugate underwent a protein self-cleavage reaction mediated by reactive oxygen species. Sequence analysis of the products indicated that cleavage occurs close in tertiary structure to the EDTA-Fe attachment site. In the presence of denaturants, the cleavage pattern changes and the reaction is limited to residues proximal in sequence to the cysteine attachment site. These results indicate that intramolecular protein cleavage reactions mediated by EDTA-Fe can be used to evaluate changes in protein conformation. The reagent described should be a useful tool in the structural mapping of nonnative protein states populated at equilibrium, such as the molten globule, that are frequently refractory to conventional structure analysis.
131 citations
TL;DR: After esterification, the thioglycuronic acids proved to be useful as both donor and acceptor in sulfonium-mediated condensations toward acidic di- and trisaccharides.
131 citations
TL;DR: In this article, the effect of Fe2+ concentration on the reduction of specific resistance was investigated in a wastewater treatment plant of An-Ping Industrial Park in Tainan, Taiwan.
131 citations