Topic
Reagent
About: Reagent is a research topic. Over the lifetime, 60091 publications have been published within this topic receiving 1234928 citations. The topic is also known as: reagens.
Papers published on a yearly basis
Papers
More filters
TL;DR: Analyses using this new scheme of an ICAT‐labeled trypsin‐digested six protein mixture as well as a tryptic digest of a total yeast lysate indicated that about two times more proteins were identified in a single analysis, and that there was no loss in accuracy of quantification.
Abstract: Quantitative protein profiling using the isotope-coded affinity tag (ICAT) method and tandem mass spectrometry (MS) enables the pair-wise comparison of protein expression levels in biological samples. A new version of the ICAT reagent with an acid-cleavable bond, which allows removal of the biotin moiety prior to MS and which utilizes (13)C substitution for (12)C in the heavy-ICAT reagent rather than (2)H (for (1)H) as in the original reagent, was investigated. We developed and validated an MS data acquisition strategy using this new reagent that results in an increased number of protein identifications per experiment, without losing the accuracy of protein quantification. This was achieved by following a single survey (precursor) ion scan and serial collision induced dissociations (CIDs) of four different precursor ions observed in the prior survey scan. This strategy is common to many high-performance liquid chromatography-electrospray ionization (HPLC-ESI)-MS shotgun proteomic strategies, but heretofore not to ICAT experiments. This advance is possible because the new ICAT reagent uses (13)C as the "heavy" element rather than (2)H, thus, eliminating the slight delay in retention time of ICAT-labeled "light" peptides on a C18-based HPLC separation that occurs with (2)H and (1)H. Analyses using this new scheme of an ICAT-labeled trypsin-digested six protein mixture as well as a tryptic digest of a total yeast lysate, indicated that about two times more proteins were identified in a single analysis, and that there was no loss in accuracy of quantification.
130 citations
TL;DR: Ferene iron reagent, 3-(2-pyridyl)-5,6-bis(2-(5-furyl sulfonic acid)-1,2,4-triazine, disodium salt, monohydrate, has been synthesized and characterized as mentioned in this paper.
Abstract: Ferene iron reagent, 3-(2-pyridyl)-5,6-bis(2-(5-furyl sulfonic acid)-1,2,4-triazine, disodium salt, monohydrate, has been synthesized and characterized. Results of a study of its complex formation ...
130 citations
TL;DR: Enol triflates react with trialkylammonium formate-palladium reagent to give alkenes as discussed by the authors, which is a reagent that can be used to synthesize polycyclic polycyclohexadecane.
130 citations
TL;DR: The results of this study showed that the feasibility of injecting Fenton's reagent or H( 2)O(2) as a Fenton-type oxidant into the subsurface is highly dependent on the soil oxidant demand (SOD), presence of sufficient quantities of ferrous iron in the application area, and the proximity of the injection area to the zone of high aqueous concentration of the target contaminant.
130 citations
TL;DR: The efficient azidation of β-keto esters and silyl enol ethers using a benziodoxole-derived azide transfer reagent is reported, achieving in up to quantitative yields in the absence of any catalyst.
130 citations