Topic
Reagent
About: Reagent is a research topic. Over the lifetime, 60091 publications have been published within this topic receiving 1234928 citations. The topic is also known as: reagens.
Papers published on a yearly basis
Papers
More filters
Patent•
10 Sep 1998TL;DR: In this paper, a method and apparatus for detecting the presence of analyte particles in a sample fluid also comprising larger particles, particularly blood, is presented, which exploits diffusion to provide simultaneous filtering of the larger particles and reaction of the analyte particle.
Abstract: This invention provides a method and apparatus for detecting the presence of analyte particles in a sample fluid also comprising larger particles, particularly blood. It exploits diffusion to provide simultaneous filtering of the larger particles and reaction of the analyte particles. A sample stream and a reagent stream join on the upstream end of a laminar flow reaction channel and flow in adjacent laminar streams. The reagents can be in solution or immobilized on a bead. The analyte particles diffuse from the sample stream into the reagent stream, leaving behind the larger particles in the residual sample stream. In the reagent stream the analyte particles react with reagent particles and form product particles, thereby creating a product stream. At the downstream end of the reaction channel, the residual sample stream and the product stream are divided. The product particles are then detected, preferably optically, in the product stream. Prior to detection, the product stream can undergo further filtering or separation, or can join a second reagent stream to form secondary product particles. This apparatus and method can be used to implement competitive immunoassays or sandwich immunoassays using enzymatically or fluoroscently labeled immunoreagents. The apparatus and method can also be used to synthesize products, in which case two reagent streams join in the laminar flow reaction channel.
128 citations
Patent•
15 May 1998
TL;DR: In this article, the presence of an analyte mediates whether or not a magnetically responsive reagent binds to a mobile solid phase reagent, or both, to the influence of a magnetic field.
Abstract: Assay methods utilizing the response of a magnetically responsive reagent to the influence of a magnetic field to qualitatively or quantitatively measure binding between specific binding pair members. According to the invention, the presence of an analyte mediates whether or not the magnetically responsive reagent binds to a mobile solid phase reagent. The extent of binding will modulate the response of the magnetically responsive reagent or that of the mobile solid phase reagent, or both, to the influence of a magnetic field. Hence, by measuring the response to the magnetic field of the magnetically responsive reagent, or that of the mobile solid phase reagent, the presence or amount of analyte contained in a test sample can accurately be determined. The invention utilizes various devices to carry out the assay methods described.
128 citations
TL;DR: Glycosyl halides serve as effectiveAlkyl halides for room temperature Negishi cross-coupling reactions using functionalized alkyl zinc reagents using in situ generated (PyBox)NiCl2.
Abstract: Glycosyl halides serve as effective alkyl halides for room temperature Negishi cross-coupling reactions using functionalized alkyl zinc reagents. The catalyst for these reactions is in situ generated (PyBox)NiCl2. The functional group tolerance on the zinc reagent is typically high, and both benzyl and ester protecting groups on the carbohydrate are tolerated. Anomer selectivities are modest for glucosyl halides but high for mannosyl halides.
127 citations
127 citations
TL;DR: The Cu(II)-Nc reagent is a milder and therefore more selective oxidant than the conventional Fe(III)-1,10-phenanthroline (phen) reagent used for the same assay, which makes the proposed method superior for real samples such as fruit juices containing weak reductants such as citrate, oxalate and tartarate that otherwise produce positive errors in the Fe( III)-phen method when equilibrium is achieved.
127 citations