Topic
Reagent
About: Reagent is a research topic. Over the lifetime, 60091 publications have been published within this topic receiving 1234928 citations. The topic is also known as: reagens.
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TL;DR: A new coupling reagent, diazonium- i -H-tetrazole, for spectrophorometric determination of histidine residues in proteins was explored and applied to several proteins and the moles of bound residues in the latter proteins were determined and discussed referring to the data obtained previously with other reagents.
124 citations
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124 citations
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124 citations
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TL;DR: A simple colorimetric method for determination of hydrogen peroxide in plant materials is described in this article, which is based on a stable red product in reaction with 4-aminoantipyrine and phenol in the presence of peroxidase.
Abstract: A simple colorimetric method for determination of hydrogen peroxide in plant materials is described. The method is based on hydrogen peroxide producing a stable red product in reaction with 4-aminoantipyrine and phenol in the presence of peroxidase. Plant tissues was ground with trichloroacetic acid (5% w/v) and extracts were adjusted to pH 8.4 with ammonia solution. Activated charcoal was added to the homogenate to remove pigments, antioxidants and other interfering substances. The colorimetric reagent (pH 5.6) consisted of 4-aminoantipyrine, phenol, and peroxidase. With this method, we have determined the hydrogen peroxide concentration in leaves of eight species which ranged from 0.2 to 0.8 µmol g−1 FW. Changes in hydrogen peroxide concentration of Stylosanthes guianensis in response to heat stress are also analyzed using this method.
124 citations
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TL;DR: In this article, the stochastic and kinetics of the reaction between various Grignard reagents and alkyl halides with an iron catalyst have been examined in tetrahydrofuran solutions.
124 citations