Topic
Reagent
About: Reagent is a research topic. Over the lifetime, 60091 publications have been published within this topic receiving 1234928 citations. The topic is also known as: reagens.
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TL;DR: Evaluation of the kinetic parameters of the various reactions involved in the determination of glutathione provided the rationale for a modification of the frequently used assay whereby the enzymatic reaction is no longer rate limiting.
185 citations
185 citations
TL;DR: A protocol is described for rapid RNA isolation from various plant species and tissues rich in polyphenolics and polysaccharides based on the Nucleon PhytoPure™ system without the use of phenol.
Abstract: A protocol is described for rapid RNA isolation from various plant species and tissues rich in polyphenolics and polysaccharides. The method is based on the Nucleon PhytoPure™ system without the use of phenol. The procedure can be completed within 1 h and many samples can be processed at the same time. The yield ranged from 240 μg up to 3 mg per gram of tissue with an average purity measured as A260/280 of 1.85. The RNA was of sufficient quality for use in RT-PCR reactions. Quantitation of single-stranded cDNA was carried out with the RiboGreen™ reagent and of PCR products with the PicoGreen™ reagent.
185 citations
TL;DR: A dimethylbarbituric acid reagent has been used to follow the kinetics of loss of two water-soluble carbodiimides, 1-ethyl-3-(3-dimethylaminopropyl) Carbodiimide (EDC) and the structurally related EAC, in aqueous solution as a function of pH and added chemical reagents.
184 citations
Patent•
27 Apr 1999
TL;DR: In this paper, a reagent for measuring the concentration of an analyte in a hemoglobin-containing biological fluid, such as whole blood, was proposed, which consisted of a flavindependent enzyme that has specificity for the analyte, a flavour cofactor if, and only if, the flavin is not bound to the enzyme, a tetrazolium dye precursor, an electron transfer agent, and a nitrite salt.
Abstract: A reagent is suitable for measuring the concentration of an analyte in a hemoglobin-containing biological fluid, such as whole blood. The reagent comprises a flavin-dependent enzyme that has specificity for the analyte, a flavin cofactor if, and only if, a flavin is not bound to the enzyme, a tetrazolium dye precursor, an electron transfer agent, and a nitrite salt. The reagent causes dye formation that is a measure of the analyte concentration. The nitrite salt suppresses interfering dye formation caused non-enzymatically by the hemoglobin. Preferably, the reagent is used in a dry strip for measuring glucose in whole blood.
184 citations